Ruqaiyyah Siddiqui , Zinb Makhlouf , Noor Akbar , Mustafa Khamis , Taleb Ibrahim , Amir Sada Khan , Naveed Ahmed Khan
{"title":"水杨酸基深度共晶溶剂的抗阿米巴特性研究——用于研制抗棘阿米巴隐形眼镜消毒液","authors":"Ruqaiyyah Siddiqui , Zinb Makhlouf , Noor Akbar , Mustafa Khamis , Taleb Ibrahim , Amir Sada Khan , Naveed Ahmed Khan","doi":"10.1016/j.molbiopara.2022.111493","DOIUrl":null,"url":null,"abstract":"<div><p><span><em>Acanthamoeba castellanii</em></span><span><span><span> is a protist pathogen that can cause sight-threatening </span>keratitis and a fatal infection of the central nervous system, known as </span>granulomatous amoebic encephalitis<span>. In this study, effects of five malonic acid and salicylic acid-based deep eutectic solvents (DES) on </span></span><em>A. castellanii</em> were investigated<em>.</em><span><span> These are salicylic acid-trioctylphosphine (DES 1), salicylic acid- trihexylamine (DES 2), salicylic acid-trioctylamine (DES 3), malonic acid-trioctylphosphine (DES 4) and malonic acid-trihexylamine (DES 5). The experiments were done by performing amoebicidal, encystment, excystment, cytopathogenicity, and </span>cytotoxicity assays. At micromolar dosage, the solvents DES 2 and DES 3 displayed significant amoebicidal effects (P < 0.05), inhibited encystment and excystment, undermined the cell-mediated cytopathogenicity of </span><em>A. castellanii,</em><span> and also displayed minimal cytotoxicity to human cells. Conversely, the chemical components of these solvents: salicylic acid<span>, trihexylamine, and trioctylamine showed minimal effects when tested individually. These results are very promising and to the best of our knowledge, are reported for the first time on the effects of deep eutectic solvents on amoebae. These results can be applied in the development of new formulations of novel contact lens disinfectants against </span></span><em>Acanthamoeba castellanii.</em></p></div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Antiamoebic properties of salicylic acid-based deep eutectic solvents for the development of contact lens disinfecting solutions against Acanthamoeba\",\"authors\":\"Ruqaiyyah Siddiqui , Zinb Makhlouf , Noor Akbar , Mustafa Khamis , Taleb Ibrahim , Amir Sada Khan , Naveed Ahmed Khan\",\"doi\":\"10.1016/j.molbiopara.2022.111493\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><em>Acanthamoeba castellanii</em></span><span><span><span> is a protist pathogen that can cause sight-threatening </span>keratitis and a fatal infection of the central nervous system, known as </span>granulomatous amoebic encephalitis<span>. In this study, effects of five malonic acid and salicylic acid-based deep eutectic solvents (DES) on </span></span><em>A. castellanii</em> were investigated<em>.</em><span><span> These are salicylic acid-trioctylphosphine (DES 1), salicylic acid- trihexylamine (DES 2), salicylic acid-trioctylamine (DES 3), malonic acid-trioctylphosphine (DES 4) and malonic acid-trihexylamine (DES 5). The experiments were done by performing amoebicidal, encystment, excystment, cytopathogenicity, and </span>cytotoxicity assays. At micromolar dosage, the solvents DES 2 and DES 3 displayed significant amoebicidal effects (P < 0.05), inhibited encystment and excystment, undermined the cell-mediated cytopathogenicity of </span><em>A. castellanii,</em><span> and also displayed minimal cytotoxicity to human cells. Conversely, the chemical components of these solvents: salicylic acid<span>, trihexylamine, and trioctylamine showed minimal effects when tested individually. These results are very promising and to the best of our knowledge, are reported for the first time on the effects of deep eutectic solvents on amoebae. These results can be applied in the development of new formulations of novel contact lens disinfectants against </span></span><em>Acanthamoeba castellanii.</em></p></div>\",\"PeriodicalId\":18721,\"journal\":{\"name\":\"Molecular and biochemical parasitology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2022-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular and biochemical parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0166685122000470\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and biochemical parasitology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0166685122000470","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Antiamoebic properties of salicylic acid-based deep eutectic solvents for the development of contact lens disinfecting solutions against Acanthamoeba
Acanthamoeba castellanii is a protist pathogen that can cause sight-threatening keratitis and a fatal infection of the central nervous system, known as granulomatous amoebic encephalitis. In this study, effects of five malonic acid and salicylic acid-based deep eutectic solvents (DES) on A. castellanii were investigated. These are salicylic acid-trioctylphosphine (DES 1), salicylic acid- trihexylamine (DES 2), salicylic acid-trioctylamine (DES 3), malonic acid-trioctylphosphine (DES 4) and malonic acid-trihexylamine (DES 5). The experiments were done by performing amoebicidal, encystment, excystment, cytopathogenicity, and cytotoxicity assays. At micromolar dosage, the solvents DES 2 and DES 3 displayed significant amoebicidal effects (P < 0.05), inhibited encystment and excystment, undermined the cell-mediated cytopathogenicity of A. castellanii, and also displayed minimal cytotoxicity to human cells. Conversely, the chemical components of these solvents: salicylic acid, trihexylamine, and trioctylamine showed minimal effects when tested individually. These results are very promising and to the best of our knowledge, are reported for the first time on the effects of deep eutectic solvents on amoebae. These results can be applied in the development of new formulations of novel contact lens disinfectants against Acanthamoeba castellanii.
期刊介绍：
The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are:
• the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances
• intermediary metabolism and bioenergetics
• drug target characterization and the mode of action of antiparasitic drugs
• molecular and biochemical aspects of membrane structure and function
• host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules.
• analysis of genes and genome structure, function and expression
• analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance.
• parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules
• parasite programmed cell death, development, and cell division at the molecular level.
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