Azam Nademi, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh
{"title":"葱载聚丙烯腈和聚乙烯吡咯烷酮纳米纤维对白色念珠菌生长及CDR1和CDR2基因表达的影响","authors":"Azam Nademi, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh","doi":"10.18502/cmm.7.4.8408","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and purpose: </strong>This study aimed to assess the effect of <i>Allium cepa</i> ethanolic extract (ACE) loaded polyacrylonitrile (PAN) and polyvinyl pyrrolidone (PVP) nanofibers on <i>Candida albicans</i> (<i>C. albicans</i>) <i>CDR1</i> and <i>CDR2</i> genes expression.</p><p><strong>Materials and methods: </strong>The minimum inhibitory concentrations (MICs) of ACE against <i>C. albicans</i> ATCC 10231 and clinical fluconazole (FLC)-resistant <i>C. albicans</i> PFCC 93-902 were determined using the Clinical and Laboratory Standards Institute (CLSI) protocol (M27-Ed4) at a concentration range of 45.3-5800 µg/ml. The nanofibers containing ACE (60 wt%) were fabricated using the electrospinning technique. The expression of the <i>CDR1</i> and <i>CDR2</i> genes was studied in the fungus exposed to ACE-loaded nanofibers and 0.5×MIC concentration of FLC using the real-time polymerase chain reaction.</p><p><strong>Results: </strong>MIC<sub>50</sub> and MIC<sub>90</sub> of ACE against FLC-resistant <i>C. albicans</i> were 725 and 1450 μg/mL, respectively. The expression of <i>CDR1</i> (4.5-fold) and <i>CDR2</i> (6.3-fold) were down-regulated after the exposure of FLC-resistant <i>C. albicans</i> to ACE-loaded nanofibers (<i>P</i><0.05). Furthermore, the expression of <i>CDR1</i> (2.8-fold) and <i>CDR2</i> (3.2-fold) were up-regulated in FLC-treated <i>C. albicans</i> (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>The results revealed that nanofibers containing ACE interact with drug-resistant genes expressed in <i>C. albicans</i>. Further studies are recommended to investigate the mode of action and other biological activities of ACE-loaded nanofibers against <i>C. albicans</i> and other pathogenic fungi.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":" ","pages":"28-33"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175147/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of <i>Allium cepa</i> loaded polyacrylonitrile and polyvinyl pyrrolidone nanofibers on <i>Candida albicans</i> growth and the expression of <i>CDR1</i> and <i>CDR2</i> genes.\",\"authors\":\"Azam Nademi, Masoomeh Shams-Ghahfarokhi, Mehdi Razzaghi-Abyaneh\",\"doi\":\"10.18502/cmm.7.4.8408\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and purpose: </strong>This study aimed to assess the effect of <i>Allium cepa</i> ethanolic extract (ACE) loaded polyacrylonitrile (PAN) and polyvinyl pyrrolidone (PVP) nanofibers on <i>Candida albicans</i> (<i>C. albicans</i>) <i>CDR1</i> and <i>CDR2</i> genes expression.</p><p><strong>Materials and methods: </strong>The minimum inhibitory concentrations (MICs) of ACE against <i>C. albicans</i> ATCC 10231 and clinical fluconazole (FLC)-resistant <i>C. albicans</i> PFCC 93-902 were determined using the Clinical and Laboratory Standards Institute (CLSI) protocol (M27-Ed4) at a concentration range of 45.3-5800 µg/ml. The nanofibers containing ACE (60 wt%) were fabricated using the electrospinning technique. The expression of the <i>CDR1</i> and <i>CDR2</i> genes was studied in the fungus exposed to ACE-loaded nanofibers and 0.5×MIC concentration of FLC using the real-time polymerase chain reaction.</p><p><strong>Results: </strong>MIC<sub>50</sub> and MIC<sub>90</sub> of ACE against FLC-resistant <i>C. albicans</i> were 725 and 1450 μg/mL, respectively. The expression of <i>CDR1</i> (4.5-fold) and <i>CDR2</i> (6.3-fold) were down-regulated after the exposure of FLC-resistant <i>C. albicans</i> to ACE-loaded nanofibers (<i>P</i><0.05). Furthermore, the expression of <i>CDR1</i> (2.8-fold) and <i>CDR2</i> (3.2-fold) were up-regulated in FLC-treated <i>C. albicans</i> (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>The results revealed that nanofibers containing ACE interact with drug-resistant genes expressed in <i>C. albicans</i>. Further studies are recommended to investigate the mode of action and other biological activities of ACE-loaded nanofibers against <i>C. albicans</i> and other pathogenic fungi.</p>\",\"PeriodicalId\":10863,\"journal\":{\"name\":\"Current Medical Mycology\",\"volume\":\" \",\"pages\":\"28-33\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9175147/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Medical Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/cmm.7.4.8408\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Medical Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/cmm.7.4.8408","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Effect of Allium cepa loaded polyacrylonitrile and polyvinyl pyrrolidone nanofibers on Candida albicans growth and the expression of CDR1 and CDR2 genes.
Background and purpose: This study aimed to assess the effect of Allium cepa ethanolic extract (ACE) loaded polyacrylonitrile (PAN) and polyvinyl pyrrolidone (PVP) nanofibers on Candida albicans (C. albicans) CDR1 and CDR2 genes expression.
Materials and methods: The minimum inhibitory concentrations (MICs) of ACE against C. albicans ATCC 10231 and clinical fluconazole (FLC)-resistant C. albicans PFCC 93-902 were determined using the Clinical and Laboratory Standards Institute (CLSI) protocol (M27-Ed4) at a concentration range of 45.3-5800 µg/ml. The nanofibers containing ACE (60 wt%) were fabricated using the electrospinning technique. The expression of the CDR1 and CDR2 genes was studied in the fungus exposed to ACE-loaded nanofibers and 0.5×MIC concentration of FLC using the real-time polymerase chain reaction.
Results: MIC50 and MIC90 of ACE against FLC-resistant C. albicans were 725 and 1450 μg/mL, respectively. The expression of CDR1 (4.5-fold) and CDR2 (6.3-fold) were down-regulated after the exposure of FLC-resistant C. albicans to ACE-loaded nanofibers (P<0.05). Furthermore, the expression of CDR1 (2.8-fold) and CDR2 (3.2-fold) were up-regulated in FLC-treated C. albicans (P<0.05).
Conclusion: The results revealed that nanofibers containing ACE interact with drug-resistant genes expressed in C. albicans. Further studies are recommended to investigate the mode of action and other biological activities of ACE-loaded nanofibers against C. albicans and other pathogenic fungi.
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