冷冻疗法可改变大鼠模型中声带皱褶的细胞外基质表达。

IF 2.5 4区医学 Q1 AUDIOLOGY & SPEECH-LANGUAGE PATHOLOGY

Journal of Voice Pub Date : 2025-03-01 DOI:10.1016/j.jvoice.2022.08.031

Ting Gong , Pengcheng Yu , Tao Lu , Jiwei Chen , Jinxia Mi , Rui Fang , Chunlei Shan

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引用次数: 0

摘要

目标：声带褶皱（VF）瘢痕是嗓音障碍的主要原因。冷冻疗法是治疗皮肤损伤的有效抗瘢痕疗法。本研究旨在探讨冷冻疗法在声带中抗瘢痕形成的潜力：方法：研究冷冻疗法对正常声带组织细胞外基质（ECM）mRNA 的表达以及冷冻疗法对声带愈合的组织学结果。对 15 只大鼠的双侧正常声带进行冷冻治疗，并分别在 1 天、3 天和 7 天时取材进行实时聚合酶链反应 (RT-PCR) 分析，以检测胶原 I、胶原 III、TGFβ1、decorin、纤连蛋白和 HAS1 的表达。10 只大鼠单侧固有层剥离损伤后立即接受或不接受冷冻治疗，2 个月后取材进行组织学和免疫组化分析：结果：冷冻治疗对 ECM 基因表达的再生效果得到了验证。组织学和免疫组化分析表明，与损伤对照组和正常对照组相比，损伤-冷冻治疗组的透明质酸明显增加，胶原减少，多聚酶沉积增加：结论：冷冻疗法可为声带组织再生提供最佳环境。本研究结果表明，冷冻疗法在预防和治疗声带瘢痕方面具有治疗潜力。

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Cryotherapy Modifies Extracellular Matrix Expression of Vocal Fold in Rat Models

Objectives

Vocal fold (VF) scarring is the major cause of voice disorders. Cryotherapy is an effective anti-scarring therapy for skin lesions. The aim of this study was to explore the anti-scarring potential of cryotherapy in vocal folds.

Methods

The extracellular matrix (ECM) mRNA expression of cryotherapy on normal VF tissue and the histologic results of cryotherapy on vocal fold healing were studied. Fifteen rats were introduced cryotherapy on the normal VF bilaterally and were harvested for real-time polymerase chain reaction (RT-PCR) analysis for collagen I, collagen III, TGFβ1, decorin, fibronectin and HAS1 at 1 day, 3 days and 7 days. Ten rats were unilaterally injured by stripping lamina propria and immediately treated with or without cryotherapy and were harvested at 2 months for histological and immunohistochemical analysis.

Results

Regenerative effect of cryotherapy was validated of ECM gene expression. Histological and immunohistochemical analysis showed significantly increased hyaluronan, decreased collagen, and increased decorin deposition in injury-cryotherapy cohort compared with injury control cohort and normal control cohort.

Conclusions

Cryotherapy may provide an optimal environment for vocal fold tissue regeneration. The results of the present investigation suggest that cryotherapy has therapeutic potential in prevention and treatment of vocal fold scarring.

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来源期刊

Journal of Voice 医学-耳鼻喉科学

CiteScore

4.00

自引率

13.60%

发文量

395

审稿时长

59 days

期刊介绍： The Journal of Voice is widely regarded as the world''s premiere journal for voice medicine and research. This peer-reviewed publication is listed in Index Medicus and is indexed by the Institute for Scientific Information. The journal contains articles written by experts throughout the world on all topics in voice sciences, voice medicine and surgery, and speech-language pathologists'' management of voice-related problems. The journal includes clinical articles, clinical research, and laboratory research. Members of the Foundation receive the journal as a benefit of membership.