微流控装置作为中枢神经系统损伤缺血模型平台,通过调节线粒体运输和生物能量代谢来研究轴突再生。

IF 4 Q2 CELL & TISSUE ENGINEERING
Ning Huang, Zu-Hang Sheng
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引用次数: 1

摘要

中枢神经系统(CNS)神经元在损伤后不能再生其轴突,导致神经功能损伤。轴突再生是一个高能量需求的细胞程序,需要局部线粒体在受损轴突内提供大部分能量。最近出现的证据线已经开始揭示损伤引发的急性线粒体损伤和局部能量危机有助于解释中枢神经系统轴突再生失败的内在能量限制。表征和重编程轴突损伤缺血后的生物能量信号和线粒体维持是制定恢复局部能量代谢从而促进轴突再生的治疗策略的基础。因此,建立可靠的、可重复的神经元模型平台对于评估损伤缺血后轴突能量代谢和再生能力至关重要。在这篇重点研究方法的文章中,我们讨论了应用尖端微流控室装置与最先进的活神经元成像工具相结合来监测成熟中枢神经系统神经元损伤缺血性应激反应中的轴突再生、线粒体运输、生物能量代谢和局部蛋白质合成的最新进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism.

Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism.

Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism.

Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism.

Central nervous system (CNS) neurons typically fail to regenerate their axons after injury leading to neurological impairment. Axonal regeneration is a highly energy-demanding cellular program that requires local mitochondria to supply most energy within injured axons. Recent emerging lines of evidence have started to reveal that injury-triggered acute mitochondrial damage and local energy crisis contribute to the intrinsic energetic restriction that accounts for axon regeneration failure in the CNS. Characterizing and reprogramming bioenergetic signaling and mitochondrial maintenance after axon injury-ischemia is fundamental for developing therapeutic strategies that can restore local energy metabolism and thus facilitate axon regeneration. Therefore, establishing reliable and reproducible neuronal model platforms is critical for assessing axonal energetic metabolism and regeneration capacity after injury-ischemia. In this focused methodology article, we discuss recent advances in applying cutting-edge microfluidic chamber devices in combination with state-of-the-art live-neuron imaging tools to monitor axonal regeneration, mitochondrial transport, bioenergetic metabolism, and local protein synthesis in response to injury-ischemic stress in mature CNS neurons.

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来源期刊
Cell Regeneration
Cell Regeneration Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
5.80
自引率
0.00%
发文量
42
审稿时长
35 days
期刊介绍: Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics: ◎ Embryonic stem cells ◎ Induced pluripotent stem cells ◎ Tissue-specific stem cells ◎ Tissue or organ regeneration ◎ Methodology ◎ Biomaterials and regeneration ◎ Clinical translation or application in medicine
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