纤毛再生需要来自纤毛基部的RNA剪接因子。

IF 4.7 Q2 CELL & TISSUE ENGINEERING
Kaiming Xu, Guangshuo Ou
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引用次数: 1

摘要

纤毛是基于微管的细胞器,从大多数真核细胞表面投射出来,执行细胞运动和信号传导。一些先前认识的非纤毛蛋白在纤毛的形成和功能中起着至关重要的作用。在这里,我们提供了额外的证据,证明秀丽隐杆线虫RNA剪接因子PRP-8/PRPF8从纤毛基部调节纤毛的发生和再生。GFP敲入动物的实时成像显示内源性PRP-8定位于细胞核和纤毛基部。一个弱功能缺失的prp-8等位基因影响纤毛结构,但对RNA剪接影响不大。PRP-8在纤毛感觉神经元内的条件降解在纤毛形成中具有直接和特异性的作用。值得注意的是,纤毛缺陷的外显率与纤毛基部而非细胞核PRP-8的减少有关,感觉神经元再生纤毛伴随着PRP-8从纤毛基部而非细胞核的恢复。我们认为纤毛基部的PRP-8有助于纤毛的形成和再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Cilia regeneration requires an RNA splicing factor from the ciliary base.

Cilia regeneration requires an RNA splicing factor from the ciliary base.

Cilia regeneration requires an RNA splicing factor from the ciliary base.

Cilia regeneration requires an RNA splicing factor from the ciliary base.

Cilia are microtubule-based organelles projected from most eukaryotic cell surfaces performing cell motility and signaling. Several previously recognized non-ciliary proteins play crucial roles in cilium formation and function. Here, we provide additional evidence that the Caenorhabditis elegans RNA splicing factor PRP-8/PRPF8 regulates ciliogenesis and regeneration from the ciliary base. Live imaging of GFP knock-in animals reveals that the endogenous PRP-8 localizes in the nuclei and the ciliary base. A weak loss-of-function allele of prp-8 affects ciliary structure but with little impact on RNA splicing. Conditional degradation of PRP-8 within ciliated sensory neurons showed its direct and specific roles in cilium formation. Notably, the penetrance of ciliary defects correlates with the reduction of PRP-8 at the ciliary base but not nuclei, and sensory neurons regenerated cilia accompanying PRP-8 recovery from the ciliary base rather than the nuclei. We suggest that PRP-8 at the ciliary base contributes to cilium formation and regeneration.

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来源期刊
Cell Regeneration
Cell Regeneration Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
5.80
自引率
0.00%
发文量
42
审稿时长
35 days
期刊介绍: Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics: ◎ Embryonic stem cells ◎ Induced pluripotent stem cells ◎ Tissue-specific stem cells ◎ Tissue or organ regeneration ◎ Methodology ◎ Biomaterials and regeneration ◎ Clinical translation or application in medicine
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