LC-MS/MS方法对浸润性导管性乳腺癌患者血清代谢组学研究。

IF 2.3 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nasrin Amiri-Dashatan, Reyhaneh Farrokhi Yekta, Mehdi Koushki, Afsaneh Arefi Oskouie, Hadi Esfahani, Salman Taheri, Elham Kazemian
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引用次数: 3

摘要

背景:浸润性导管癌(Invasive ductal carcinoma, IDC)是最常见的乳腺癌类型,早期发现可显著降低死亡率。然而,IDC的预后因素并不充分,我们需要新的标记物来治疗不同的个体。虽然正电子发射断层扫描和磁共振成像技术是可用的,但它们是基于形态特征,不能提供任何线索的分子事件伴随癌症的进展。近年来,“组学”方法得到了广泛的发展,提出了新的癌症分子特征作为假定的生物标志物,特别是在生物流体中。因此,我们进行了基于质谱的代谢组学研究,以寻找IDC的一些可能的代谢物标记物和与雌激素受体、孕激素受体、淋巴血管侵袭和人表皮生长因子受体2相关的潜在代谢物,这些代谢物具有预后价值。方法:采用液相色谱-串联质谱(LC-MS/MS)对IDC患者进行非靶向代谢组学研究。XCMS在线多元主成分分析建立了一个模型,可以分离研究组并定义显著改变的m/z参数。通过途径富集分析确定了最重要的生物学途径。结果:IDC血清样品中代谢产物发生显著变化的主要是氨基酸和脂类。最重要的相关途径包括精氨酸和脯氨酸代谢、甘油磷脂代谢以及苯丙氨酸、酪氨酸和色氨酸的生物合成。结论:与健康对照相比,IDC血清样本中代谢物的显著改变可能导致基于代谢物的潜在生物标志物的发展,这是在其他方法和大型队列中得到证实的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolomic study of serum in patients with invasive ductal breast carcinoma with LC-MS/MS approach.

Background: Invasive ductal carcinoma (IDC) is the most common type of breast cancer so its early detection can lead to a significant decrease in mortality rate. However, prognostic factors for IDC are not adequate and we need novel markers for the treatment of different individuals. Although positron emission tomography and magnetic resonance imaging techniques are available, they are based on morphological features that do not provide any clue for molecular events accompanying cancer progression. In recent years, "omics" approaches have been extensively developed to propose novel molecular signatures of cancers as putative biomarkers, especially in biofluids. Therefore, a mass spectrometry-based metabolomics investigation was performed to find some putative metabolite markers of IDC and potential metabolites with prognostic value related to the estrogen receptor, progesterone receptor, lymphovascular invasion, and human epidermal growth factor receptor 2.

Methods: An untargeted metabolomics study of IDC patients was performed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The multivariate principal component analysis by XCMS online built a model that could separate the study groups and define the significantly altered m/z parameters. The most important biological pathways were also identified by pathway enrichment analysis.

Results: The results showed that the significantly altered metabolites in IDC serum samples mostly belonged to amino acids and lipids. The most important involved pathways included arginine and proline metabolism, glycerophospholipid metabolism, and phenylalanine, tyrosine, and tryptophan biosynthesis.

Conclusions: Significantly altered metabolites in IDC serum samples compared to healthy controls could lead to the development of metabolite-based potential biomarkers after confirmation with other methods and in large cohorts.

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来源期刊
International Journal of Biological Markers
International Journal of Biological Markers 医学-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
43
期刊介绍: IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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