Inês Rosado Vitorino , Dominika Klimek , Magdalena Calusinska , Alexandre Lobo-da-Cunha , Vítor Vasconcelos , Olga Maria Lage
{"title":"Rhodopirellula aestuarii sp. nov.，从葡萄牙塔霍河河口收集的微咸沉积物中分离出来的Rhodopirellula属新成员","authors":"Inês Rosado Vitorino , Dominika Klimek , Magdalena Calusinska , Alexandre Lobo-da-Cunha , Vítor Vasconcelos , Olga Maria Lage","doi":"10.1016/j.syapm.2022.126360","DOIUrl":null,"url":null,"abstract":"<div><p>Bacteria within the phylum <em>Planctomycetota</em><span> are biologically relevant due to unique characteristics among prokaryotes. Members of the genus </span><span><em>Rhodopirellula</em></span><span> can be abundant in marine habitats, however, only six species are currently validly described. In this study, we expand the explored genus diversity by formally describing a novel species. The pink-coloured strain ICT_H3.1</span><sup>T</sup><span> was isolated from brackish sediments collected in the Tagus estuary<span> (Portugal) and a 16S rRNA gene sequence-based analysis placed this strain into the genus </span></span><em>Rhodopirellula</em> (family <em>Pirellulaceae</em>). The closest type strain is <em>Rhodopirellula rubra</em> LF2<sup>T</sup>, suggested by a similarity of 98.4% of the 16S rRNA gene sequence. Strain ICT_H3.1<sup>T</sup> is heterotrophic, aerobic and able to grow under microaerobic conditions. The strain grows between 15 and 37 °C, over a range of pH 6.5 to 11.0 and from 1 to 8% (w/v) NaCl. Several nitrogen and carbon sources were utilized by the novel isolate. Cells have an elongated pear-shape with 2.0 ± 0.3 × 0.9 ± 0.2 µm in size. Cells of strain ICT_H3.1<sup>T</sup><span> cluster in rosettes through a holdfast<span><span> structure and divide by budding. Younger cells are motile. Ultrathin cell sections show cytoplasmic membrane invaginations and polar </span>fimbriae<span><span>. The genome size is 9,072,081 base pairs with a </span>DNA G + C content of 56.1 mol%. Genomic, physiological and morphological comparison of strain ICT_H3.1</span></span></span><sup>T</sup> with its relatives suggest that it belongs to a novel species within the genus <em>Rhodopirellula</em>. Hence, we propose the name <em>Rhodopirellula aestuarii</em> sp. nov., represented by ICT_H3.1<sup>T</sup> (=CECT30431<sup>T</sup> = LMG32464<sup>T</sup>) as the type strain of this novel species.</p><p><strong>16S rRNA gene accession number:</strong> GenBank = OK001858.</p><p><strong>Genome accession number:</strong> The Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAMQBK000000000. The version described in this paper is version JAMQBK010000000.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Rhodopirellula aestuarii sp. nov., a novel member of the genus Rhodopirellula isolated from brackish sediments collected in the Tagus River estuary, Portugal\",\"authors\":\"Inês Rosado Vitorino , Dominika Klimek , Magdalena Calusinska , Alexandre Lobo-da-Cunha , Vítor Vasconcelos , Olga Maria Lage\",\"doi\":\"10.1016/j.syapm.2022.126360\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Bacteria within the phylum <em>Planctomycetota</em><span> are biologically relevant due to unique characteristics among prokaryotes. Members of the genus </span><span><em>Rhodopirellula</em></span><span> can be abundant in marine habitats, however, only six species are currently validly described. In this study, we expand the explored genus diversity by formally describing a novel species. The pink-coloured strain ICT_H3.1</span><sup>T</sup><span> was isolated from brackish sediments collected in the Tagus estuary<span> (Portugal) and a 16S rRNA gene sequence-based analysis placed this strain into the genus </span></span><em>Rhodopirellula</em> (family <em>Pirellulaceae</em>). The closest type strain is <em>Rhodopirellula rubra</em> LF2<sup>T</sup>, suggested by a similarity of 98.4% of the 16S rRNA gene sequence. Strain ICT_H3.1<sup>T</sup> is heterotrophic, aerobic and able to grow under microaerobic conditions. The strain grows between 15 and 37 °C, over a range of pH 6.5 to 11.0 and from 1 to 8% (w/v) NaCl. Several nitrogen and carbon sources were utilized by the novel isolate. Cells have an elongated pear-shape with 2.0 ± 0.3 × 0.9 ± 0.2 µm in size. Cells of strain ICT_H3.1<sup>T</sup><span> cluster in rosettes through a holdfast<span><span> structure and divide by budding. Younger cells are motile. Ultrathin cell sections show cytoplasmic membrane invaginations and polar </span>fimbriae<span><span>. The genome size is 9,072,081 base pairs with a </span>DNA G + C content of 56.1 mol%. Genomic, physiological and morphological comparison of strain ICT_H3.1</span></span></span><sup>T</sup> with its relatives suggest that it belongs to a novel species within the genus <em>Rhodopirellula</em>. Hence, we propose the name <em>Rhodopirellula aestuarii</em> sp. nov., represented by ICT_H3.1<sup>T</sup> (=CECT30431<sup>T</sup> = LMG32464<sup>T</sup>) as the type strain of this novel species.</p><p><strong>16S rRNA gene accession number:</strong> GenBank = OK001858.</p><p><strong>Genome accession number:</strong> The Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAMQBK000000000. The version described in this paper is version JAMQBK010000000.</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202022000674\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202022000674","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Rhodopirellula aestuarii sp. nov., a novel member of the genus Rhodopirellula isolated from brackish sediments collected in the Tagus River estuary, Portugal
Bacteria within the phylum Planctomycetota are biologically relevant due to unique characteristics among prokaryotes. Members of the genus Rhodopirellula can be abundant in marine habitats, however, only six species are currently validly described. In this study, we expand the explored genus diversity by formally describing a novel species. The pink-coloured strain ICT_H3.1T was isolated from brackish sediments collected in the Tagus estuary (Portugal) and a 16S rRNA gene sequence-based analysis placed this strain into the genus Rhodopirellula (family Pirellulaceae). The closest type strain is Rhodopirellula rubra LF2T, suggested by a similarity of 98.4% of the 16S rRNA gene sequence. Strain ICT_H3.1T is heterotrophic, aerobic and able to grow under microaerobic conditions. The strain grows between 15 and 37 °C, over a range of pH 6.5 to 11.0 and from 1 to 8% (w/v) NaCl. Several nitrogen and carbon sources were utilized by the novel isolate. Cells have an elongated pear-shape with 2.0 ± 0.3 × 0.9 ± 0.2 µm in size. Cells of strain ICT_H3.1T cluster in rosettes through a holdfast structure and divide by budding. Younger cells are motile. Ultrathin cell sections show cytoplasmic membrane invaginations and polar fimbriae. The genome size is 9,072,081 base pairs with a DNA G + C content of 56.1 mol%. Genomic, physiological and morphological comparison of strain ICT_H3.1T with its relatives suggest that it belongs to a novel species within the genus Rhodopirellula. Hence, we propose the name Rhodopirellula aestuarii sp. nov., represented by ICT_H3.1T (=CECT30431T = LMG32464T) as the type strain of this novel species.
Genome accession number: The Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JAMQBK000000000. The version described in this paper is version JAMQBK010000000.
期刊介绍：
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
