米曲霉作为靛蓝素(一种具有抗氧化活性的非核糖体肽色素)生物合成平台的潜力。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2022-06-23 eCollection Date: 2022-01-01 DOI:10.1371/journal.pone.0270359
Sarocha Panchanawaporn, Chanikul Chutrakul, Sukanya Jeennor, Jutamas Anantayanon, Nakul Rattanaphan, Kobkul Laoteng
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引用次数: 2

摘要

工业部门对天然色素不断增长的需求是生产工艺发展的重要推动力。利用微生物系统生产具有广泛工业用途的天然蓝色色素已引起人们的广泛关注。本研究以米曲霉(Aspergillus oryzae)为平台细胞工厂,通过对其非核糖体肽合成酶系统进行遗传操作,使其过表达靛蓝素合成酶基因(AoinK),生产蓝色色素靛蓝素(InK)。表型分析表明,由于基因表达的组成控制,工程菌株的InK产量与生长相关。此外,初始pH、温度、谷氨酰胺和MgSO4浓度是影响工程菌株生产油墨的关键因素。在培养基中加入1%的Tween 80溶液,可促进色素分泌。总InK的最大效价为1409.22±95.33 mg/L,最大产率为265.09±14.74 mg/L·d。此外,工程菌株产生的重组墨水具有抗氧化活性。这些结果表明,a.m oryzae有潜力作为真菌平台过度生产细胞外非核糖体肽色素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Potential of Aspergillus oryzae as a biosynthetic platform for indigoidine, a non-ribosomal peptide pigment with antioxidant activity.

Potential of Aspergillus oryzae as a biosynthetic platform for indigoidine, a non-ribosomal peptide pigment with antioxidant activity.

Potential of Aspergillus oryzae as a biosynthetic platform for indigoidine, a non-ribosomal peptide pigment with antioxidant activity.

Potential of Aspergillus oryzae as a biosynthetic platform for indigoidine, a non-ribosomal peptide pigment with antioxidant activity.

The growing demand for natural pigments in the industrial sector is a significant driving force in the development of production processes. The production of natural blue pigments, which have wide industrial applications, using microbial systems has been gaining significant attention. In this study, we used Aspergillus oryzae as a platform cell factory to produce the blue pigment indigoidine (InK), by genetic manipulation of its non-ribosomal peptide synthetase system to overexpress the indigoidine synthetase gene (AoinK). Phenotypic analysis showed that InK production from the engineered strain was growth associated, owing to the constitutive control of gene expression. Furthermore, the initial pH, temperature, and glutamine and MgSO4 concentrations were key factors affecting InK production by the engineered strain. The pigment secretion was enhanced by addition of 1% Tween 80 solution to the culture medium. The maximum titer of total InK was 1409.22 ± 95.33 mg/L, and the maximum productivity was 265.09 ± 14.74 mg/L·d. Moreover, the recombinant InK produced by the engineered strain exhibited antioxidant activity. These results indicate that A. oryzae has the potential to be used as a fungal platform for overproduction of extracellular non-ribosomal peptide pigments.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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