Hayrunnisa Oral, Zafer Türkyılmaz, Ramazan Karabulut, Cem Kaya, Duygu Dayanır, Cengiz Karakaya, Kaan Sonmez
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Groups 3, 4, and 5 constituted the study groups in which volvulus was created in a 5-cm- long ileal segment 2 cm proximal to the ileocecal valve. After 2 hours the volvuli were reduced and following 2 hours of reperfusion, these segments were removed. In volvulus-I/R group (Group 3) no additional procedure was done. HRSS was administered shortly before reperfusion (reduction of the volvulus) in Treatment I (Group 4) and 1 h before experimental volvulus in Treatment II (Group 5) groups. Blood and intestinal tissue samples were obtained from all rats at the 4th hour. Both tissue and blood total oxidant (TOS) and antioxidant status (TAS) levels were determined and tissue histomorphologies were studied. Oxidative stress indices (TOS ÷ TAS) (OSI) were calculated.</p><p><strong>Results: </strong>Tissue TOS and OSI levels and histomorphological injury scores were statistically lower in treatment groups than I/R group, whereas blood TOS and OSI levels were similar between the groups.</p><p><strong>Conclusions: </strong>This study provides biochemical and histomorphological evidence that HRSS prevents intestinal damage in I/R injury caused by volvulus.</p>","PeriodicalId":16200,"journal":{"name":"Journal of Investigative Surgery","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protective Effects of Hydrogen-Rich Saline on Experimental Intestinal Volvulus in Rats.\",\"authors\":\"Hayrunnisa Oral, Zafer Türkyılmaz, Ramazan Karabulut, Cem Kaya, Duygu Dayanır, Cengiz Karakaya, Kaan Sonmez\",\"doi\":\"10.1080/08941939.2022.2056273\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Intestinal volvulus can cause morbidity and mortality. 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引用次数: 0
摘要
背景:肠扭转可引起发病率和死亡率。另一方面,手术复位可能导致缺血再灌注(I/R)损伤。富氢盐溶液(HRSS)可以中和体内的自由基。本研究旨在探讨HRSS在大鼠实验性肠扭转I/R损伤中的作用。方法:30只大鼠随机分为5组。所有实验均在全身麻醉和无菌条件下进行。Sham组(组1)和HRSS组(组2)分别腹腔注射5 ml/kg生理盐水和HRSS。第3、4、5组为回肠回盲瓣近端2cm处长5cm的回肠段扭转组。2小时后将扭转缩小,再灌注2小时后将这些节段切除。肠扭转- i /R组(第3组)未行其他手术。第1组(第4组)在再灌注(减少扭转)前不久给予HRSS,第2组(第5组)在实验性扭转前1 h给予HRSS。第4小时取大鼠血液和肠组织标本。测定大鼠组织和血液总氧化剂(TOS)和抗氧化状态(TAS)水平,并研究组织组织形态学。计算氧化应激指数(TOS ÷ TAS) (OSI)。结果:治疗组组织TOS、OSI水平及组织形态学损伤评分均低于I/R组,而血液TOS、OSI水平组间比较相似。结论:本研究提供了HRSS预防肠扭转I/R损伤的生化和组织形态学证据。
Protective Effects of Hydrogen-Rich Saline on Experimental Intestinal Volvulus in Rats.
Background: Intestinal volvulus can cause morbidity and mortality. Surgical reduction, on the other hand, could result in ischemia-reperfusion (I/R) injury. Hydrogen rich saline solution (HRSS neutralizes free radicals in the body. This study aimed to investigate the effects of HRSS in I/R injury in experimental intestinal volvulus in rats.
Methods: Thirty rats were randomly allocated into 5 groups. All procedures were done under general anesthesia and sterile conditions in each animal. Five ml/kg of saline and HRSS were administered intraperitoneally (ip) in Sham (Group 1) and HRSS (Group 2) groups, respectively. Groups 3, 4, and 5 constituted the study groups in which volvulus was created in a 5-cm- long ileal segment 2 cm proximal to the ileocecal valve. After 2 hours the volvuli were reduced and following 2 hours of reperfusion, these segments were removed. In volvulus-I/R group (Group 3) no additional procedure was done. HRSS was administered shortly before reperfusion (reduction of the volvulus) in Treatment I (Group 4) and 1 h before experimental volvulus in Treatment II (Group 5) groups. Blood and intestinal tissue samples were obtained from all rats at the 4th hour. Both tissue and blood total oxidant (TOS) and antioxidant status (TAS) levels were determined and tissue histomorphologies were studied. Oxidative stress indices (TOS ÷ TAS) (OSI) were calculated.
Results: Tissue TOS and OSI levels and histomorphological injury scores were statistically lower in treatment groups than I/R group, whereas blood TOS and OSI levels were similar between the groups.
Conclusions: This study provides biochemical and histomorphological evidence that HRSS prevents intestinal damage in I/R injury caused by volvulus.
期刊介绍:
Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.