OSGIN2对骨质疏松大鼠颌骨间充质干细胞成骨的调控作用。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Yi Shuai, Bingyao Liu, Liang Rong, Bingyi Shao, Bo Chen, Lei Jin
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引用次数: 1

摘要

背景:雌激素缺乏后氧化应激增强导致颌骨骨髓间充质干细胞(BMSCs)功能不足,导致骨质疏松症患者颌骨丢失。OSGIN2是一种氧化应激诱导因子,被发现与骨骼疾病有关。本研究旨在探讨OSGIN2在骨质疏松大鼠颌骨间充质干细胞中的作用。使用颌骨骨髓间充质干细胞。结果:骨质疏松大鼠颌骨骨髓间充质干细胞氧化应激升高,OSGIN2表达上调。氧化应激导致颌骨骨髓间充质干细胞成骨能力下降,而沉默OSGIN2可改善成骨缺陷。氧化应激下,rora及其下游成骨标志物(BSP、OCN)表达降低,而OSGIN2下调后,其表达恢复。抑制OSGIN2可促进氧化应激下颌骨BMSCs的成骨,而下调RORα可抵消这一作用。骨内灌注si-OSGIN2可挽救骨质疏松大鼠颌骨缺损,促进新骨沉积。结论:氧化应激在骨质疏松症中是冗余的,导致OSGIN2上调。OSGIN2通过调控RORα抑制颌骨骨髓间充质干细胞成骨能力,而OSGIN2沉默可缓解骨质疏松大鼠成骨缺陷。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

OSGIN2 regulates osteogenesis of jawbone BMSCs in osteoporotic rats.

OSGIN2 regulates osteogenesis of jawbone BMSCs in osteoporotic rats.

OSGIN2 regulates osteogenesis of jawbone BMSCs in osteoporotic rats.

OSGIN2 regulates osteogenesis of jawbone BMSCs in osteoporotic rats.

Background: Augmentation of oxidative stress after estrogen deficiency leading to functional deficiency of jawbone bone marrow mesenchymal stem cells (BMSCs) causes jawbone loss in osteoporosis. OSGIN2, an oxidative stress induced factor, has been found to be associated with skeletal diseases. This study aims to investigate the function of OSGIN2 in jawbone BMSCs of osteoporotic rats. Jawbone BMSCs were used.

Results: Oxidative stress was increased in jawbone BMSCs of osteoporotic rats, meanwhile OSGIN2 was also up-regulated. Osteogenesis of jawbone BMSCs was declined under oxidative stress, while silence of OSGIN2 ameliorated the osteogenic deficiency. RORα and its downstream osteogenic markers (BSP and OCN) decreased under oxidative stress, while knocking-down of OSGIN2 restored their expressions. Inhibition of OSGIN2 improved the osteogenesis of jawbone BMSCs under oxidative stress, whereas down-regulation of RORα offset the effect. Intra-jawbone infusion of si-OSGIN2 rescued jawbone loss and promoted new bone deposition of osteoporotic rats.

Conclusions: Oxidative stress is redundant in osteoporosis, which results in up-regulation of OSGIN2. OSGIN2 restricts osteogenic ability of jawbone BMSCs via regulating RORα, while silencing of OSGIN2 rescues the osteogenic deficiency of osteoporotic rats.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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