{"title":"血流感染中大卵氏菌的鉴定及药敏研究。","authors":"Xiangyun Li, Enjun Xu, Cuixiao Shi, Guanhua Qiao, Shuyi Chen, Yuanhong Xu, Yajing Liu, Xundi Bao","doi":"10.33073/pjm-2022-024","DOIUrl":null,"url":null,"abstract":"<p><p>The identification and antibiotic susceptibility of two clinical isolates of <i>Eggerthella lenta</i> from bloodstream infections were determined. This microorganism is rarely pathogenic, and the findings are presented here to promote the detection and awareness of this infection. The bacteria were obtained from one patient with pressure sores and another with a malignant gastric tumor. Smears were prepared, stained, and examined by microscopy. Single colonies were analyzed by Gram staining, MALDI-TOF MS, and the 16S rRNA gene sequencing. Antibiotic sensitivity was assessed by the agar dilution method. The bacilli were found to be Gram-positive, and the MS results showed 99.8% homology with <i>E. lenta</i>. It was confirmed by gene sequencing. Antibiotic susceptibility tests demonstrated that <i>E. lenta</i> was sensitive to piperacillin-tazobactam, ampicillin-sulbactam, imipenem, meropenem, metronidazole, clindamycin, and vancomycin. This study could increase awareness of this rare infection.</p>","PeriodicalId":20272,"journal":{"name":"Polish Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2022-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/4d/pjm-71-257.PMC9252136.pdf","citationCount":"0","resultStr":"{\"title\":\"Identification and Antibiotic Susceptibility of <i>Eggerthella lenta</i> in Bloodstream Infections.\",\"authors\":\"Xiangyun Li, Enjun Xu, Cuixiao Shi, Guanhua Qiao, Shuyi Chen, Yuanhong Xu, Yajing Liu, Xundi Bao\",\"doi\":\"10.33073/pjm-2022-024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The identification and antibiotic susceptibility of two clinical isolates of <i>Eggerthella lenta</i> from bloodstream infections were determined. This microorganism is rarely pathogenic, and the findings are presented here to promote the detection and awareness of this infection. The bacteria were obtained from one patient with pressure sores and another with a malignant gastric tumor. Smears were prepared, stained, and examined by microscopy. Single colonies were analyzed by Gram staining, MALDI-TOF MS, and the 16S rRNA gene sequencing. Antibiotic sensitivity was assessed by the agar dilution method. The bacilli were found to be Gram-positive, and the MS results showed 99.8% homology with <i>E. lenta</i>. It was confirmed by gene sequencing. Antibiotic susceptibility tests demonstrated that <i>E. lenta</i> was sensitive to piperacillin-tazobactam, ampicillin-sulbactam, imipenem, meropenem, metronidazole, clindamycin, and vancomycin. This study could increase awareness of this rare infection.</p>\",\"PeriodicalId\":20272,\"journal\":{\"name\":\"Polish Journal of Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2022-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/4d/pjm-71-257.PMC9252136.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish Journal of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2022-024\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish Journal of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.33073/pjm-2022-024","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Identification and Antibiotic Susceptibility of Eggerthella lenta in Bloodstream Infections.
The identification and antibiotic susceptibility of two clinical isolates of Eggerthella lenta from bloodstream infections were determined. This microorganism is rarely pathogenic, and the findings are presented here to promote the detection and awareness of this infection. The bacteria were obtained from one patient with pressure sores and another with a malignant gastric tumor. Smears were prepared, stained, and examined by microscopy. Single colonies were analyzed by Gram staining, MALDI-TOF MS, and the 16S rRNA gene sequencing. Antibiotic sensitivity was assessed by the agar dilution method. The bacilli were found to be Gram-positive, and the MS results showed 99.8% homology with E. lenta. It was confirmed by gene sequencing. Antibiotic susceptibility tests demonstrated that E. lenta was sensitive to piperacillin-tazobactam, ampicillin-sulbactam, imipenem, meropenem, metronidazole, clindamycin, and vancomycin. This study could increase awareness of this rare infection.
期刊介绍:
Polish Journal of Microbiology (PJM) publishes original research articles describing various aspects of basic and applied microbiological research. We are especially interested in articles regarding
- basic biological properties of bacteria and archaea, viruses, and simple
- eukaryotic microorganisms
- genetics and molecular biology
- microbial ecology
- medical bacteriology and public health
- food microbiology
- industrial microbiology
- bacterial biotechnology