拟南芥叶源性和根源性细胞昼夜行为的适应性多样化。

Shunji Nakamura, Tokitaka Oyama
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引用次数: 0

摘要

植物的昼夜节律系统是基于自我维持的细胞振荡,并被用来适应日常和季节性的环境变化。植物地上、地下器官的细胞生物钟受到不同的局部环境的影响。在植物中,单个细胞时钟受到其他细胞/组织的影响,单个细胞的内在昼夜节律特性仍有待阐明。在这项研究中,我们监测了来自CCA1::LUC转基因拟南芥叶片和根系的单个原生质体来源细胞的生物发光昼夜节律。我们分析了叶源细胞和根源细胞的昼夜节律特性,并证明了与其他细胞没有物理接触的细胞具有真正的昼夜节律时钟,具有~ 24小时的周期性,可携带性和周期的温度补偿。节律的稳定性与细胞密度有关。高细胞密度导致叶源性细胞的昼夜节律改善,而这种效应与细胞节律之间的相位关系无关。对单个细胞生物发光节律的定量和统计分析揭示了叶源细胞和根源细胞在光/暗夹带的振幅和精度上的差异。在细胞水平上,叶和根的昼夜节律系统是多样化的,以适应当地的环境。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adaptive Diversification in the Cellular Circadian Behavior of Arabidopsis Leaf- and Root-Derived Cells.

The plant circadian system is based on self-sustained cellular oscillations and is utilized to adapt to daily and seasonal environmental changes. The cellular circadian clocks in the above- and belowground plant organs are subjected to diverse local environments. Individual cellular clocks are affected by other cells/tissues in plants, and the intrinsic circadian properties of individual cells remain to be elucidated. In this study, we monitored bioluminescence circadian rhythms of individual protoplast-derived cells from leaves and roots of a CCA1::LUC Arabidopsis transgenic plant. We analyzed the circadian properties of the leaf- and root-derived cells and demonstrated that the cells with no physical contact with other cells harbor a genuine circadian clock with ∼24-h periodicity, entrainability and temperature compensation of the period. The stability of rhythm was dependent on the cell density. High cell density resulted in an improved circadian rhythm of leaf-derived cells while this effect was observed irrespective of the phase relation between cellular rhythms. Quantitative and statistical analyses for individual cellular bioluminescence rhythms revealed a difference in amplitude and precision of light/dark entrainment between the leaf- and root-derived cells. Circadian systems in the leaves and roots are diversified to adapt to their local environments at the cellular level.

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