带蒂胃壁皮瓣上肠粘膜生长的新技术。

IF 2.1 4区 医学 Q2 SURGERY
Journal of Investigative Surgery Pub Date : 2022-06-01 Epub Date: 2022-02-23 DOI:10.1080/08941939.2022.2034196
Panagiotis Sakarellos, Apostolos Papalois, Harikleia Gakiopoulou, Iro Zacharioudaki, Michalis Katsimpoulas, Marina Belia, Dimitrios Moris, Kyveli Aggelou, Ilias Vagios, Spiridon Davakis, Michail Vailas, Theodoros Liakakos, Theodoros Diamantis, Evangelos Felekouras, Michael Kontos
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引用次数: 0

摘要

背景:短肠综合征(SBS)在现代医学中仍是一个未解决的问题。为了提高肠道的吸收能力,已经提出了许多实验手术技术。材料与方法:10头长白猪,分为A组和B组,每组5头,经中线切口探查。一个梭形血管化全层胃壁皮瓣(GWF),由部分主弯曲组成,保留胃大网膜弓,然后作为“贴片”放置,覆盖无功能盲肠袢(A组)或胃肠道功能肠袢(B组)的反肠系膜边界缺陷。a组在补片外表面缝合低密度聚乙烯(LDPE)夹板,防止GWF收缩和肠壁塌陷。结果:补片的两个尺寸均减小。显微镜下,一层薄薄的柱状上皮细胞覆盖在斑块的中心,形成较短的、钝的、发育不良的绒毛,并逐渐向外侧成熟。斑块表面被覆盖了近90%。在三个过早死亡的动物中,GWF的覆盖是疏忽的或次优的,直接依赖于生存时间的长短。结论:本文所述的补片技术显示了肠道新粘膜在GWF上的生长。胃提供大皮瓣的能力和使用天然组织的优势使该动物模型对该领域的未来研究具有价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Growth of Intestinal Neomucosa on Pedicled Gastric Wall Flap, a Novel Technique in an Animal Model.

Background: Short bowel syndrome (SBS) remains an unsolved issue in modern medicine. Numerous experimental surgical techniques have been proposed in the attempt to increase the intestinal absorptive capacity.Materials and Methods: Ten female Landrace pigs, divided in two groups of 5 (A and B), were explored through a midline incision. A spindle-shaped vascularized full-thickness gastric wall flap (GWF) consisting of part of the major curvature with the gastroepiploic arch preserved was de-epithelialized and then placed as a "patch" to cover an antimesenteric border defect of either a nonfunctional blind intestinal loop (group A) or a functional intestinal loop of the gastrointestinal tract (group B). A spindle-shaped curved, rigid, low density polyethylene (LDPE) splint was sutured on the external surface of the patch in order to prevent shrinkage of GWF and collapse of the intestinal wall in group A.Results: There was a decrease of both dimensions of the patch. Microscopically a thin layer of columnar epithelial cells covered the center of the patch, evolving in shorter, blunt, poorly developed villi with increasing maturation laterally. The patch surface was covered by nearly 90%. In the three animals that died prematurely the coverage of GWF was negligent or suboptimal directly dependent on the length of survival.Conclusions: The hereby-described patching technique demonstrated the growth of intestinal neomucosa on the GWF. The capability of the stomach to provide large flaps and the advantages of the use of native tissues render this animal model valuable for the future research in the field.

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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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