马睾丸对维生素D生物活化作用的研究

IF 1.9 2区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Marion Rouge , Razan Elkhatib , Christelle Delalande , Juliette Cognié , Fabrice Reigner , Philippe Barriere , Stefan Deleuze , Matthieu Cousty , Florence Legendre , Philippe Galera , Vincent Hanoux , Hélène Bouraima-Lelong
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引用次数: 1

摘要

尽管维生素D在多种生物过程中起作用,但它在维持骨骼健康和调节钙稳态方面起着关键作用。在人类和啮齿类动物中,参与维生素D代谢的主要组织是肝脏和肾脏,但研究表明,睾丸也强烈地参与了维生素D的生物激活。事实上,在这些不同的物种中,代谢维生素D的酶(CYP27A1, CYP27B1和CYP2R1)已在该组织中得到证实。此外,患有性腺功能减退症的男性体内维生素d的循环水平也有所下降。在马类中,阉割雄性是一种常规做法,以减少被认为过于好斗的种马的行为。阉割在不同的年龄进行:在马驹生长期间或在成年后,一旦他们达到了他们的最佳尺寸。尽管马表现出非典型的维生素D代谢和低循环水平的维生素D,但有人认为睾丸可能有助于其激活,正如在啮齿动物和人类中所描述的那样;因此,阉割可能会影响它的新陈代谢。在这项研究中,在阉割前和阉割后,在不同年龄测量了维生素D (1 α,25[OH] 2维生素d3)的生物活性形式的血液水平:1周,青春期后(2年)和成年后(6年)。参与维生素D代谢的酶的基因表达已经在不同实验组的睾丸中被寻找。无论手术时的年龄如何,去势后的生物活性维生素D3水平没有变化。无论睾丸发育如何,马的特殊地位与睾丸对维生素D代谢的贡献低或缺乏有关。参与维生素D生物活化的酶发出的信号较低或缺乏表明了这一点。因此,马在比较内分泌学中构成了一个独特的模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of equine testis contribution to vitamin D bioactivation

Although vitamin D acts in various biological processes, it plays a critical role in the maintenance of bone health, and regulates calcium homeostasis. In humans and rodents, the main tissues involved in vitamin D metabolism are the liver and the kidneys, however it has been shown that the testis has strongly participated in its bioactivation. Indeed, in these different species, enzymes metabolizing vitamin D (CYP27A1, CYP27B1 and CYP2R1) have been demonstrated in this tissue. Moreover, men with hypogonadism have shown a decrease in circulating levels of vitamin D. In equine species, the castration of males is a regular practice to reduce the behavior of stallions deemed too aggressive. Castration is carried out at various ages: in foals during their growth or in adulthood once they have reached their optimum size. Although horses exhibit atypical vitamin D metabolism with low circulating levels of vitamin D, it was suggested that testis may contribute to its activation as has been described in rodents and humans; castration could therefore be likely to affect its metabolism. In this study, blood levels of bioactive form of vitamin D (1 α,25[OH] 2 vitamin D 3 ) were measured before and after castration at different ages: 1 wk, after puberty (2 yr) and at adulthood (6 yr). The gene expression of enzymes involved in vitamin D metabolism has been sought in the testis of different experimental groups. No change in bioactive vitamin D3 levels was observed after castration regardless of the age at the time of surgery. The exceptional status of equine species is confirmed with a low or a lack of testis contribution to vitamin D metabolism, regardless of testicular development. This is demonstrated by a low or a lack of signal from enzymes involved in vitamin D bioactivation. Therefore, horses constitute a unique model in comparative endocrinology.

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来源期刊
Domestic animal endocrinology
Domestic animal endocrinology 农林科学-奶制品与动物科学
CiteScore
5.50
自引率
4.80%
发文量
58
审稿时长
31 days
期刊介绍: Domestic Animal Endocrinology publishes scientific papers dealing with the study of the endocrine physiology of domestic animal species. Those manuscripts utilizing other species as models for clinical or production problems associated with domestic animals are also welcome. Topics covered include: Classical and reproductive endocrinology- Clinical and applied endocrinology- Regulation of hormone secretion- Hormone action- Molecular biology- Cytokines- Growth factors
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