液体分枝杆菌培养物的再净化:在南非开普敦的Xpert MTB/RIF Ultra时代的额外结核分枝杆菌产量。

IF 1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

African Journal of Laboratory Medicine Pub Date : 2021-12-10 eCollection Date: 2021-01-01 DOI:10.4102/ajlm.v10i1.1529

Dawood da Costa, Pieter Nel

{"title":"液体分枝杆菌培养物的再净化:在南非开普敦的Xpert MTB/RIF Ultra时代的额外结核分枝杆菌产量。","authors":"Dawood da Costa, Pieter Nel","doi":"10.4102/ajlm.v10i1.1529","DOIUrl":null,"url":null,"abstract":"A retrospective review of liquid mycobacterial cultures was performed at a laboratory in South Africa from 01 January 2018 to 31 December 2018 to assess the increased yield in detecting Mycobacterium tuberculosis complex following sample re-decontamination. Only 9 of 99 (9%) re-decontaminated samples were culture positive for M. tuberculosis complex. Xpert MTB/RIF Ultra, concurrently performed on 7 of the 9 samples, detected M. tuberculosis complex in all but 1 sample. Re-decontamination of non-sterile samples did not increase the M. tuberculosis complex yield enough to offset the financial costs and additional labour in a laboratory that utilises the Xpert MTB/RIF Ultra system as a first-line diagnostic modality.","PeriodicalId":45412,"journal":{"name":"African Journal of Laboratory Medicine","volume":"10 1","pages":"1529"},"PeriodicalIF":1.0000,"publicationDate":"2021-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678946/pdf/","citationCount":"0","resultStr":"{\"title\":\"Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa.\",\"authors\":\"Dawood da Costa, Pieter Nel\",\"doi\":\"10.4102/ajlm.v10i1.1529\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A retrospective review of liquid mycobacterial cultures was performed at a laboratory in South Africa from 01 January 2018 to 31 December 2018 to assess the increased yield in detecting Mycobacterium tuberculosis complex following sample re-decontamination. Only 9 of 99 (9%) re-decontaminated samples were culture positive for M. tuberculosis complex. Xpert MTB/RIF Ultra, concurrently performed on 7 of the 9 samples, detected M. tuberculosis complex in all but 1 sample. Re-decontamination of non-sterile samples did not increase the M. tuberculosis complex yield enough to offset the financial costs and additional labour in a laboratory that utilises the Xpert MTB/RIF Ultra system as a first-line diagnostic modality.\",\"PeriodicalId\":45412,\"journal\":{\"name\":\"African Journal of Laboratory Medicine\",\"volume\":\"10 1\",\"pages\":\"1529\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2021-12-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8678946/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"African Journal of Laboratory Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4102/ajlm.v10i1.1529\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Journal of Laboratory Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4102/ajlm.v10i1.1529","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

摘要

2018年1月1日至2018年12月31日，在南非的一个实验室对液体分枝杆菌培养物进行了回顾性审查，以评估样品重新净化后结核分枝杆菌复体检出率的提高。99份再净化样本中只有9份(9%)结核分枝杆菌复合体培养阳性。Xpert MTB/RIF Ultra同时对9份样品中的7份进行检测，除1份样品外，其余样品均检测到结核分枝杆菌复合体。对非无菌样品进行再消毒并没有使结核分枝杆菌复体产量增加到足以抵消使用Xpert MTB/RIF Ultra系统作为一线诊断方式的实验室的财务成本和额外劳动力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Re-decontamination of liquid mycobacterial cultures: Additional Mycobacterium tuberculosis yield in the era of Xpert MTB/RIF Ultra in Cape Town, South Africa.

A retrospective review of liquid mycobacterial cultures was performed at a laboratory in South Africa from 01 January 2018 to 31 December 2018 to assess the increased yield in detecting Mycobacterium tuberculosis complex following sample re-decontamination. Only 9 of 99 (9%) re-decontaminated samples were culture positive for M. tuberculosis complex. Xpert MTB/RIF Ultra, concurrently performed on 7 of the 9 samples, detected M. tuberculosis complex in all but 1 sample. Re-decontamination of non-sterile samples did not increase the M. tuberculosis complex yield enough to offset the financial costs and additional labour in a laboratory that utilises the Xpert MTB/RIF Ultra system as a first-line diagnostic modality.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

African Journal of Laboratory Medicine MEDICINE, RESEARCH & EXPERIMENTAL-

CiteScore

1.70

自引率

9.10%

发文量

审稿时长

12 weeks

期刊介绍： The African Journal of Laboratory Medicine, the official journal of ASLM, focuses on the role of the laboratory and its professionals in the clinical and public healthcare sectors,and is specifically based on an African frame of reference. Emphasis is on all aspects that promote and contribute to the laboratory medicine practices of Africa. This includes, amongst others: laboratories, biomedical scientists and clinicians, medical community, public health officials and policy makers, laboratory systems and policies (translation of laboratory knowledge, practices and technologies in clinical care), interfaces of laboratory with medical science, laboratory-based epidemiology, laboratory investigations, evidence-based effectiveness in real world (actual) settings.