Minjung Chae, Beom Joon Kim, Jungtae Na, Su-Young Kim, Jung Ok Lee, Yu-Jin Kim, Esther Lee, Donghyun Cho, Jonghwa Roh, Wangi Kim
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In the present study, we investigated the potential antimicrobial activity of <i>Lactiplantibacillus plantarum</i> APsulloc 331261 (APsulloc 331261) and <i>Lactiplantibacillus plantarum</i> APsulloc 331266 (APsulloc 331266) derived from green tea, in inhibiting five skin pathogenic strains (<i>Staphylococcus aureus</i> (<i>S. aureus</i>), <i>Cutibacterium acnes</i> (<i>C. acnes</i>), <i>Candia albicans</i> (<i>C. albicans</i>), <i>Malassezia globosa</i> (<i>M. globose</i>), and <i>Malassezia restricta</i> (<i>M. restricta</i>)) associated with skin infection. Viability of <i>S. aureus</i>, <i>C. acnes</i>, <i>C. albicans</i>, <i>M. globosa</i>, and <i>M. restricta</i> was inhibited by indirect co-culture with APsulloc 331261 or APsulloc 331266 at various ratios. Different concentrations of the cell-free conditioned media (CM) derived from APsulloc 331261 or APsulloc 331266 inhibited the vaibility of <i>S. aureus</i>, <i>C. acnes</i>, <i>C. albicans</i>, <i>M. globosa</i>, and <i>M. restricta</i> in a dose dependent manner. Moreover, susceptibility of <i>S. aureus</i>, <i>C. acnes</i> and <i>C. albicans</i> against APsulloc 331261 or APsulloc 331266 was confirmed following agar overlay methods. Results of the agar overlay confirmed that various concentrations of APsulloc 331261 and APsulloc 331266 exhibited low to high inhibitory activity on the growth of <i>S. aureus</i> (ZDI 20.3 ± 2.1-32.3 ± 2.1 mm, R value 5.7 ± 0.8-7.8 ± 1.3 mm), <i>C. acnes</i> (ZDI 15.0 ± 1.7-22.2 ± 1.7 mm, R value 3.2 ± 1.3-5.5 ± 1.3 mm) and <i>C. albicans</i> (ZDI 13.3 ± 4.0-27.0 ± 3.6 mm, R value 2.8 ± 1.9-5.5 ± 1.7 mm). Finally, standard PCR analysis identified the presence of the of plantaricin genes encoding antimicrobial peptides in APsulloc 331261 and APsulloc 331266. These results suggest that APsulloc 331261 and APsulloc 331266 has a potential effect in the improvement of the balance of skin microbiota by inhibiting skin pathogenic strains.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":"13 2","pages":"237-248"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Antimicrobial activity of <i>Lactiplantibacillus plantarum</i> APsulloc 331261 and APsulloc 331266 against pathogenic skin microbiota.\",\"authors\":\"Minjung Chae, Beom Joon Kim, Jungtae Na, Su-Young Kim, Jung Ok Lee, Yu-Jin Kim, Esther Lee, Donghyun Cho, Jonghwa Roh, Wangi Kim\",\"doi\":\"10.52586/E881\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Balanced skin microbiota is crucial for maintaining healthy normal skin function; however, disruption of the balance in skin microbiota is linked with skin diseases such as atopic dermatitis, acne vulgaris, dandruff, and candidiasis. <i>Lactoplantibacillus</i> species with proved with health benefits are probiotics that improve the balance of microbiome in skin and gut. In the present study, we investigated the potential antimicrobial activity of <i>Lactiplantibacillus plantarum</i> APsulloc 331261 (APsulloc 331261) and <i>Lactiplantibacillus plantarum</i> APsulloc 331266 (APsulloc 331266) derived from green tea, in inhibiting five skin pathogenic strains (<i>Staphylococcus aureus</i> (<i>S. aureus</i>), <i>Cutibacterium acnes</i> (<i>C. acnes</i>), <i>Candia albicans</i> (<i>C. albicans</i>), <i>Malassezia globosa</i> (<i>M. globose</i>), and <i>Malassezia restricta</i> (<i>M. restricta</i>)) associated with skin infection. Viability of <i>S. aureus</i>, <i>C. acnes</i>, <i>C. albicans</i>, <i>M. globosa</i>, and <i>M. restricta</i> was inhibited by indirect co-culture with APsulloc 331261 or APsulloc 331266 at various ratios. Different concentrations of the cell-free conditioned media (CM) derived from APsulloc 331261 or APsulloc 331266 inhibited the vaibility of <i>S. aureus</i>, <i>C. acnes</i>, <i>C. albicans</i>, <i>M. globosa</i>, and <i>M. restricta</i> in a dose dependent manner. Moreover, susceptibility of <i>S. aureus</i>, <i>C. acnes</i> and <i>C. albicans</i> against APsulloc 331261 or APsulloc 331266 was confirmed following agar overlay methods. Results of the agar overlay confirmed that various concentrations of APsulloc 331261 and APsulloc 331266 exhibited low to high inhibitory activity on the growth of <i>S. aureus</i> (ZDI 20.3 ± 2.1-32.3 ± 2.1 mm, R value 5.7 ± 0.8-7.8 ± 1.3 mm), <i>C. acnes</i> (ZDI 15.0 ± 1.7-22.2 ± 1.7 mm, R value 3.2 ± 1.3-5.5 ± 1.3 mm) and <i>C. albicans</i> (ZDI 13.3 ± 4.0-27.0 ± 3.6 mm, R value 2.8 ± 1.9-5.5 ± 1.7 mm). Finally, standard PCR analysis identified the presence of the of plantaricin genes encoding antimicrobial peptides in APsulloc 331261 and APsulloc 331266. These results suggest that APsulloc 331261 and APsulloc 331266 has a potential effect in the improvement of the balance of skin microbiota by inhibiting skin pathogenic strains.</p>\",\"PeriodicalId\":73068,\"journal\":{\"name\":\"Frontiers in bioscience (Elite edition)\",\"volume\":\"13 2\",\"pages\":\"237-248\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in bioscience (Elite edition)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.52586/E881\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in bioscience (Elite edition)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.52586/E881","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
摘要
平衡的皮肤微生物群对维持健康正常的皮肤功能至关重要;然而，皮肤微生物群平衡的破坏与皮肤疾病有关，如特应性皮炎、寻常性痤疮、头皮屑和念珠菌病。乳植物杆菌是一种被证明对健康有益的益生菌，可以改善皮肤和肠道微生物群的平衡。在本研究中，我们研究了从绿茶中提取的植物乳杆菌胶囊331261(胶囊331261)和植物乳杆菌胶囊331266(胶囊331266)对5种皮肤致病菌株(金黄色葡萄球菌(S. aureus)、痤疮表皮杆菌(C. acnes)、白色念珠菌(C. albicans)、球形马拉色菌(M. globose)和限制性马拉色菌(M. restricta))的潜在抑菌活性。与不同比例的胶囊331261或胶囊331266间接共培养可抑制金黄色葡萄球菌、痤疮葡萄球菌、白色念珠菌、球形芽孢杆菌和限制性芽孢杆菌的生存能力。不同浓度的由胶囊331261或胶囊331266衍生的无细胞条件培养基(CM)以剂量依赖的方式抑制金黄色葡萄球菌、痤疮葡萄球菌、白色念珠菌、球形念珠菌和限制性念珠菌的活性。此外，采用琼脂覆盖法证实了金黄色葡萄球菌、痤疮葡萄球菌和白色念珠菌对胶囊331261或胶囊331266的敏感性。琼脂覆盖结果证实，不同浓度的胶囊331261和胶囊331266对金黄色葡萄球菌(ZDI为20.3±2.1 ~ 32.3±2.1 mm, R值为5.7±0.8 ~ 7.8±1.3 mm)、痤疮芽孢杆菌(ZDI为15.0±1.7 ~ 22.2±1.7 mm, R值为3.2±1.3 ~ 5.5±1.3 mm)和白色念珠菌(ZDI为13.3±4.0 ~ 27.0±3.6 mm, R值为2.8±1.9 ~ 5.5±1.7 mm)的生长均表现出低至高的抑制活性。最后，通过标准PCR分析确定了在胶囊331261和胶囊331266中存在编码抗菌肽的plantaricin基因。上述结果提示，胶囊331261和胶囊331266可能通过抑制皮肤病原菌，改善皮肤菌群平衡。
Antimicrobial activity of Lactiplantibacillus plantarum APsulloc 331261 and APsulloc 331266 against pathogenic skin microbiota.
Balanced skin microbiota is crucial for maintaining healthy normal skin function; however, disruption of the balance in skin microbiota is linked with skin diseases such as atopic dermatitis, acne vulgaris, dandruff, and candidiasis. Lactoplantibacillus species with proved with health benefits are probiotics that improve the balance of microbiome in skin and gut. In the present study, we investigated the potential antimicrobial activity of Lactiplantibacillus plantarum APsulloc 331261 (APsulloc 331261) and Lactiplantibacillus plantarum APsulloc 331266 (APsulloc 331266) derived from green tea, in inhibiting five skin pathogenic strains (Staphylococcus aureus (S. aureus), Cutibacterium acnes (C. acnes), Candia albicans (C. albicans), Malassezia globosa (M. globose), and Malassezia restricta (M. restricta)) associated with skin infection. Viability of S. aureus, C. acnes, C. albicans, M. globosa, and M. restricta was inhibited by indirect co-culture with APsulloc 331261 or APsulloc 331266 at various ratios. Different concentrations of the cell-free conditioned media (CM) derived from APsulloc 331261 or APsulloc 331266 inhibited the vaibility of S. aureus, C. acnes, C. albicans, M. globosa, and M. restricta in a dose dependent manner. Moreover, susceptibility of S. aureus, C. acnes and C. albicans against APsulloc 331261 or APsulloc 331266 was confirmed following agar overlay methods. Results of the agar overlay confirmed that various concentrations of APsulloc 331261 and APsulloc 331266 exhibited low to high inhibitory activity on the growth of S. aureus (ZDI 20.3 ± 2.1-32.3 ± 2.1 mm, R value 5.7 ± 0.8-7.8 ± 1.3 mm), C. acnes (ZDI 15.0 ± 1.7-22.2 ± 1.7 mm, R value 3.2 ± 1.3-5.5 ± 1.3 mm) and C. albicans (ZDI 13.3 ± 4.0-27.0 ± 3.6 mm, R value 2.8 ± 1.9-5.5 ± 1.7 mm). Finally, standard PCR analysis identified the presence of the of plantaricin genes encoding antimicrobial peptides in APsulloc 331261 and APsulloc 331266. These results suggest that APsulloc 331261 and APsulloc 331266 has a potential effect in the improvement of the balance of skin microbiota by inhibiting skin pathogenic strains.
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