{"title":"AlliGator:用于快速体内寿命分析的相位计算平台。","authors":"Sez-Jade Chen, Nattawut Sinsuebphon, Margarida Barroso, Xavier Intes, Xavier Michalet","doi":"10.1364/omp.2017.omtu2d.2","DOIUrl":null,"url":null,"abstract":"<p><p>A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET <i>in vitro</i> and <i>in vivo</i>. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.</p>","PeriodicalId":74365,"journal":{"name":"Optical molecular probes, imaging and drug delivery","volume":"2017 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2017-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772506/pdf/nihms-1693534.pdf","citationCount":"0","resultStr":"{\"title\":\"AlliGator: A Phasor Computational Platform for Fast <i>in vivo</i> Lifetime Analysis.\",\"authors\":\"Sez-Jade Chen, Nattawut Sinsuebphon, Margarida Barroso, Xavier Intes, Xavier Michalet\",\"doi\":\"10.1364/omp.2017.omtu2d.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET <i>in vitro</i> and <i>in vivo</i>. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.</p>\",\"PeriodicalId\":74365,\"journal\":{\"name\":\"Optical molecular probes, imaging and drug delivery\",\"volume\":\"2017 \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772506/pdf/nihms-1693534.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Optical molecular probes, imaging and drug delivery\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1364/omp.2017.omtu2d.2\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optical molecular probes, imaging and drug delivery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1364/omp.2017.omtu2d.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
AlliGator: A Phasor Computational Platform for Fast in vivo Lifetime Analysis.
A fast time-gated phasor analysis tool was developed and used to monitor kinetics of NIR FLIM-FRET in vitro and in vivo. The results were validated by comparison with standard two-component fluorescence decay fitting analysis.