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Since current genetic engineering technique relies on viral vectors, issues about immunogenicity, insertional oncogenesis, retention, and targeted delivery remain unanswered. The use of nanotechnology has the potential to improve the safety and efficacy of CRISPR/Cas9 component distribution by employing tailored polymeric nanoparticles. The combination of two (CRISPR/Cas9 and nanotechnology) offers the potential to open new therapeutic paths. Considering the benefits, demand, and constraints, the goal of this research is to acquire more about the biology of CRISPR technology, as well as aspects of selective and effective diagnostics and therapies for infectious illnesses and other metabolic disorders. This review advocated combining nanomedicine (nanomedicine) with a CRISPR/Cas enabled sensing system to perform early-stage diagnostics and selective therapy of specific infectious disorders. Such a Nano-CRISPR-powered nanomedicine and sensing system would allow for successful infectious illness control, even on a personal level. 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引用次数: 0
摘要
随后，生物医学研究人员受到启发，开发出了精确改变生物体基因组 DNA 的新方法，以便利用基因工程技术研究定制诊断和治疗方法。聚类调控间隔短联合重复序列（CRISPR）就是这样一种技术，它已成为一种安全、有针对性和有效的药物治疗方法，可用于治疗包括细菌、真菌、寄生虫和病毒在内的多种致病生物以及基因异常。最近发现的非常灵活的工程核酸结合蛋白以革命性的方式改变了基因组编辑这一科学领域。由于目前的基因工程技术依赖于病毒载体，因此有关免疫原性、插入致癌、保留和靶向传递等问题仍未得到解决。纳米技术的使用有可能通过采用定制的聚合物纳米颗粒来提高 CRISPR/Cas9 成分分布的安全性和有效性。两者（CRISPR/Cas9 和纳米技术）的结合有可能开辟新的治疗途径。考虑到CRISPR技术的益处、需求和制约因素，本研究的目标是进一步了解CRISPR技术的生物学特性，以及针对传染性疾病和其他代谢性疾病的选择性有效诊断和疗法的各个方面。这篇综述主张将纳米医学（nanomedicine）与 CRISPR/Cas 传感系统结合起来，对特定感染性疾病进行早期诊断和选择性治疗。这种由纳米-CRISPR 驱动的纳米医学和传感系统可以成功控制传染病，甚至是个人传染病。本综合研究还讨论了该预测技术目前存在的障碍和潜力：在线版本包含补充材料，可查阅 10.1007/s40097-022-00472-7。
Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases.
Biomedical researchers have subsequently been inspired the development of new approaches for precisely changing an organism's genomic DNA in order to investigate customized diagnostics and therapeutics utilizing genetic engineering techniques. Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one such technique that has emerged as a safe, targeted, and effective pharmaceutical treatment against a wide range of disease-causing organisms, including bacteria, fungi, parasites, and viruses, as well as genetic abnormalities. The recent discovery of very flexible engineered nucleic acid binding proteins has changed the scientific area of genome editing in a revolutionary way. Since current genetic engineering technique relies on viral vectors, issues about immunogenicity, insertional oncogenesis, retention, and targeted delivery remain unanswered. The use of nanotechnology has the potential to improve the safety and efficacy of CRISPR/Cas9 component distribution by employing tailored polymeric nanoparticles. The combination of two (CRISPR/Cas9 and nanotechnology) offers the potential to open new therapeutic paths. Considering the benefits, demand, and constraints, the goal of this research is to acquire more about the biology of CRISPR technology, as well as aspects of selective and effective diagnostics and therapies for infectious illnesses and other metabolic disorders. This review advocated combining nanomedicine (nanomedicine) with a CRISPR/Cas enabled sensing system to perform early-stage diagnostics and selective therapy of specific infectious disorders. Such a Nano-CRISPR-powered nanomedicine and sensing system would allow for successful infectious illness control, even on a personal level. This comprehensive study also discusses the current obstacles and potential of the predicted technology.
Graphical abstract:
Supplementary information: The online version contains supplementary material available at 10.1007/s40097-022-00472-7.
期刊介绍：
The Journal of Nanostructure in Chemistry (JNC) publishes cutting-edge research at the intersections of chemistry, biology, biotechnology, materials science, physics, and engineering. It features high-quality research, perspectives, and review articles covering various disciplines within the natural sciences, biomedicine, and engineering. The journal's scope includes, but is not limited to, the following topics:
Target drug and gene delivery
Tissue engineering and regenerative medicine
Cancer therapy
Diagnosis and Bioimaging
Electrochemical detection and sensing
Food industry and packaging
Environments (catalyst, coatings, and water treatment)
Energy (fuel cells, capacitor, laser)
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