使用68Ga-EDTA PET可以检测大鼠肾功能的进行性下降。

IF 2 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
American journal of nuclear medicine and molecular imaging Pub Date : 2021-12-15 eCollection Date: 2021-01-01
Andrea O Fontana, Mary Gonzalez Melo, Gilles Allenbach, Costa Georgantas, Ruijia Wang, Olivier Braissant, Frederic Barbey, John O Prior, Diana Ballhausen, David Viertl
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引用次数: 0

摘要

评价肾小球滤过率在临床前和临床中都非常重要,特别是在慢性肾脏疾病的背景下。它通常使用51Cr-EDTA或123I-Hippuran显像进行成像,与PET相比,其分辨率和灵敏度明显较低。68Ga- edta是一种有效的替代方案,因为它可以使用68Ge/68Ga发生器快速获得,而PET/CT既可以成像肾功能,又可以准确定量血浆和尿液中的活性清除。因此,我们的目的是研究使用68Ga-EDTA作为临床前示踪剂,在已知存在肾功能进行性下降的敲入大鼠模型中测定肾功能。方法:68Ga-EDTA注射野生型(n=10)和敲入型(n=13)大鼠23只。采用单向双室模型和Rutland-Patlak Plot线性回归分析,测定6周龄至12个月的分裂肾功能。结果:野生型大鼠肾小球滤过6周时为0.025±0.01 ml/min, 6个月时为0.049±0.05 ml/min。敲入大鼠在6个月和12个月时肾小球滤过明显降低(p结论:68Ga-EDTA是一种非常有前途的PET/CT示踪剂,用于评估分裂肾功能。这种方法可以在敲入大鼠模型中检测进行性肾损害。有必要在人类队列中进一步验证,以进一步评估健康个体和肾脏损害患者的临床效用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The use of <sup>68</sup>Ga-EDTA PET allows detecting progressive decline of renal function in rats.

The use of <sup>68</sup>Ga-EDTA PET allows detecting progressive decline of renal function in rats.

The use of 68Ga-EDTA PET allows detecting progressive decline of renal function in rats.

Introduction: Evaluation of glomerular filtration rate is very important in both preclinical and clinical setting, especially in the context of chronic kidney disease. It is typically performed using 51Cr-EDTA or by imaging with 123I-Hippuran scintigraphy, which has a significantly lower resolution and sensitivity as compared to PET. 68Ga-EDTA represents a valid alternative due to its quick availability using a 68Ge/68Ga generator, while PET/CT enables both imaging of renal function and accurate quantitation of clearance of activity from both plasma and urine. Therefore, we aimed at investigating the use of 68Ga-EDTA as a preclinical tracer for determining renal function in a knock-in rat model known to present progressive decline of renal function.

Methods: 68Ga-EDTA was injected in 23 rats, either wild type (n=10) or knock-in (n=13). By applying a unidirectional, two-compartment model and Rutland-Patlak Plot linear regression analysis, split renal function was determined from the age of 6 weeks to 12 months.

Results: Glomerular filtration ranged from 0.025±0.01 ml/min at 6 weeks to 0.049±0.05 ml/min at 6 months in wild type rats. Glomerular filtration was significantly lower in knock-in rats at 6 and 12 months (P<0.01). No significant difference was observed in renal volumes between knock-in and wild type animals, based on imaging-derived volume calculations.

Conclusions: 68Ga-EDTA turned out to be a very promising PET/CT tracer for the evaluation of split renal function. This method allowed detection of progressive renal impairment in a knock-in rat model. Additional validation in a human cohort is warranted to further assess clinical utility in both, healthy individuals and patients with renal impairment.

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来源期刊
American journal of nuclear medicine and molecular imaging
American journal of nuclear medicine and molecular imaging RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
自引率
4.00%
发文量
4
期刊介绍: The scope of AJNMMI encompasses all areas of molecular imaging, including but not limited to: positron emission tomography (PET), single-photon emission computed tomography (SPECT), molecular magnetic resonance imaging, magnetic resonance spectroscopy, optical bioluminescence, optical fluorescence, targeted ultrasound, photoacoustic imaging, etc. AJNMMI welcomes original and review articles on both clinical investigation and preclinical research. Occasionally, special topic issues, short communications, editorials, and invited perspectives will also be published. Manuscripts, including figures and tables, must be original and not under consideration by another journal.
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