Сharacteristics细胞周期蛋白d1介导的对切尔诺贝利清理工人和口腔、口咽、喉咽恶性肿瘤患者外周血淋巴细胞周期的调节。

N A Golyarnik, I N Ilienko, L M Zvarych, M O Vorobyov, D A Bazyka
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引用次数: 0

摘要

目的:通过细胞周期蛋白D1的表达水平及细胞周期的定量参数,探讨切尔诺贝利清理工人及口腔、口咽、喉咽恶性肿瘤患者外周血淋巴细胞的增殖潜能。材料与方法:调查了294例男性(58.47±7.32),其中215例为切尔诺贝利清理工人(1986 ~ 1987),暴露剂量范围为10.43 ~ 3623.31 mSv;对照组49人,口腔、口咽、喉咽恶性肿瘤患者30人,分别处于疾病的III期、IVА期和IVВ期。采用流式细胞术分析外周血淋巴细胞的细胞周期参数和增殖活性。在体内和体外分别用G0/G1、S、G2/M细胞周期评价细胞分布。通过细胞周期蛋白D1的表达水平分析细胞增殖潜能。结果:评价了切尔诺贝利清理工人及口腔、口咽、喉咽部恶性肿瘤患者血浆中PB淋巴细胞的增殖潜能。在切尔诺贝利清理工人中,测定了mitto "gen激活后,自发表达水平的增加和依赖于 cyclin D1的PB淋巴细胞周期调节的紊乱。在细胞周期的S期和G2/M期细胞池数量增加,表明PB淋巴细胞具有较高的增殖潜力。这些变化在辐射剂量D > 500毫西弗的人群亚组和肿瘤病理患者中最为明显。结论:在辐射剂量> 500 mSv的切尔诺贝利清理工人亚群中,辐射剂量与细胞周期S期细胞数量呈线性正相关。在辐射暴露后的一段较长时间内,检测到细胞周期蛋白d1依赖的细胞周期调节和淋巴细胞增殖状态的变化取决于辐射剂量,可能是基因组不稳定的一种表现,也是致癌风险的一个原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
СHARACTERISTICS OF CYCLIN D1-MEDIATED REGULATION OF CELL CYCLE OF PERIPHERAL BLOOD LIMPHOCYTES OF CHORNOBYL CLEAN-UP WORKERS AND PERSONS WITH MALIGNANT NEOPLASMS OF THE ORAL CAVITY, OROPHARYNX AND LARYNGOPHARYNX.

Objective: to explore proliferative potential of peripheral blood lymphocytes of Chornobyl clean-up workers and persons with malignant neoplasms of the oral cavity, oropharynx and laryngopharynx by level of expression of cyclin D1 and quantitative parameters of cell cycle.

Materials and methods: A total of 294 men aged (58.47 ± 7.32) were surveyed, 215 of them were Chornobyl clean"up workers (1986-1987), exposed at the dose range 10.43-3623.31 mSv; 49 persons of the control group and 30persons with malignant neoplasms of the oral cavity, oropharynx and laryngopharynx at III, IVА and IVВ stages ofthe disease. The analysis of parameters of cell cycle and proliferative activity of peripheral blood (PB) lymphocyteswas performed using the flow cytometry. The evaluation of distribution of cells by G0/G1, S, G2/M cell cycle phaseswas done in vivo and in in vitro. Proliferative potential was analyzed by level of expression of cytoplasmic protein ofcyclin D1.

Results: Proliferative potential of PB lymphocytes of Chornobyl clean"up workers and persons with malignant neo"plasms of the oral cavity, oropharynx and laryngopharynx was assessed. An increase in the level of spontaneousсyclin D1 expression and disturbance of сyclin D1-dependent regulation of cell cycle of PB lymphocytes after mito"gen activation were determined in the Chornobyl clean-up workers. An increase in pool of cells in the S" and G2/M"phases of cell cycle was detected, which characterizes high proliferative potential of PB lymphocytes. These changesare most pronounced in the subgroup of persons with a radiation dose of D > 500 mSv, and in persons with oncolo"gical pathology.

Conclusions: A positive linear dependence has been established between the radiation dose and the number of cellsin the S"phase of cell cycle in the subgroup of Chornobyl clean"up workers with a radiation dose of D > 500 mSv. The detected changes of cyclin D1-dependent regulation of cell cycle and proliferative status of lymphocytes depend on the radiation dose, can be a manifestation of genome instability and be a cause for risks of oncogenesis, in a remote period after radiation exposure.

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Problemy radiatsiinoi medytsyny ta radiobiolohii
Problemy radiatsiinoi medytsyny ta radiobiolohii Medicine-Radiology, Nuclear Medicine and Imaging
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