Guang-Min Yu, Li-Feng Zhou, Bi-Xia Zeng, Jing-Jun Huang, Xiao-Jun She
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引用次数: 0
摘要
背景:类风湿性关节炎(RA)作为一种慢性自身免疫性疾病,与氧化应激有关,氧化应激可能导致RA炎症的发生和持续。本研究旨在评估三苯氧胺在胶原诱导的关节炎(CIA)大鼠模型中的潜在抗氧化作用:方法:我们检测了接受曲普内酯治疗的 CIA 大鼠的关节炎严重程度、局部和全身氧化应激水平、关节周围骨侵蚀和器官重量:结果:我们发现三苯氧胺能显著降低大鼠爪厚度和临床关节炎评分。三苯氧胺治疗后,CIA 大鼠爪部髓过氧化物酶和诱导型一氧化氮合酶的 mRNA 表达和活性明显降低。雷公藤内酯能明显抑制血清中亚硝酸盐和硝酸盐的含量以及尿液中地屈嗪的含量。曲托内酯还能明显增加胫骨的骨量,但抑制股骨和胫骨的骺板厚度。此外,治疗后除脾脏重量下降外,其他器官重量无明显差异:这些结果表明,CIA 大鼠的局部和全身氧化应激增强,而治疗剂量的曲普内酯具有明确的抗氧化作用。
The antioxidant effect of triptolide contributes to the therapy in a collagen-induced arthritis rat model.
Background: As a chronic autoimmune disease, rheumatoid arthritis (RA) is related to oxidative stress, which may lead to the occurrence and persistence of inflammation in RA. The purpose of this study is to evaluate the potential antioxidant effect of triptolide in collagen-induced arthritis (CIA) rat model.
Methods: We examined the severity of arthritis, levels of local and systemic oxidative stress, periarticular bone erosion and weight of organs in CIA rats treated with triptolide.
Results: We found that triptolide decreased the paw thickness and clinical arthritis score, significantly. The mRNA expression and activity of myeloperoxidase and inducible nitric oxide synthase were remarkably decreased in the paws of the CIA rats after triptolide treatment. Triptolide significantly inhibited the levels of nitrite and nitrate in serum, as well as the urinary level of dityrosine. Triptolide treatment also markedly increased bone volume of tibia, but suppressed epiphyseal plate thickness of both femur and tibia. In addition, there was no significant difference in the weight of organs after the therapy, except decreased spleen weight.
Conclusions: These results suggested that the local and systemic oxidative stress was enhanced in the CIA rats and the therapeutic dose of triptolide had a definite antioxidant effect.
期刊介绍:
Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included.
While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.