正常和肥胖大鼠神经挤压损伤后周围神经和脂肪组织干细胞的潜在作用。

IF 2.1 4区 医学 Q2 SURGERY
Journal of Investigative Surgery Pub Date : 2022-05-01 Epub Date: 2022-01-30 DOI:10.1080/08941939.2021.1991530
Elif Kayhan Kustepe, Berrin Zuhal Altunkaynak, Işınsu Alkan, Elfide Gizem Kivrak, Alişan Yildiran, Stefano Geuna
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引用次数: 0

摘要

本研究的目的是探讨来自周围神经和脂肪组织的干细胞对正常和肥胖大鼠神经挤压损伤的影响。为此,41只Wistar白化雌性大鼠被分成8组;非肥胖对照组(NOC)、肥胖对照组(OC)、非肥胖损伤组(NOH)、肥胖损伤组(OH)、非肥胖脂肪组(NOY)、肥胖脂肪组(OY)、非肥胖神经组(NOPS)、肥胖神经组(OPS)。8周结束时,不设对照组的所有实验动物均行神经挤压术,治疗组大鼠注射坐骨神经或脂肪干细胞匀浆,观察恢复过程,并对切除的坐骨神经进行组织病理学、体视学、电生理分析和生物信息学评价。体视学结果显示,与NOPS组相比,NOY组脂肪匀浆比周围神经匀浆在髓鞘轴突数量方面取得了更成功的结果。与OY组相比,OPS组的周围神经匀浆显示出更成功的结果。在脂肪组织匀浆处理后,NOY组和OY组无髓鞘轴突数量增加。在髓鞘厚度方面;我们发现外周神经,特别是脂肪组织匀浆处理导致外周神经轴突厚度增加,属于正常和肥胖损伤组。结果表明,新鲜组织匀浆间充质干细胞治疗周围神经再生是成功的,脂肪组织是临床应用的重要干细胞来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potential Effects of Stem Cells Derived from the Peripheral Nerve and Adipose Tissue after the Nerve Crush Injury in Control and Obese Rats.

Aim of this study is to investigate effects of stem cells derived from the peripheral nerve and adipose tissues following the nerve crush injury in control and obese rats. For this aim, 41 Wistar Albino female rats were separated into eight equal groups; non-obese control (NOC) obese control (OC), non-obese injury (NOH), obese injury (OH), non-obese adipose (NOY), obese adipose (OY), non-obese nerve (NOPS), obese nerve (OPS). At the end of 8 weeks, all experimental animals without control groups were subjected to nerve crush procedure and sciatic nerve or fat stem cell homogenates were injected on the treatment group rats, and then, recovery process has been observed and histopathological, stereological, electrophysiological analyses and bioinformatic evaluation were made on removed sciatic nerves. Stereological results showed that adipose homogenate gave more successful results than peripheral nerve homogenates in the NOY group in comparison to the NOPS group in terms of myelinated axon number. Peripheral nerve homogenate has shown more successful results in the OPS group in comparison to the OY group. The number of unmyelinated axons was increased following treatment with adipose tissue homogenate in NOY and OY groups. In terms of myelin sheath thickness; we detected that treatments by peripheral nerve and especially adipose tissue homogenates lead to increase in the thickness of the axons of the peripheral nerves belong to the control and obese injury groups. All results showed that mesenchymal stem cell treatment by fresh tissue homogenates is successful in peripheral nerve regeneration and fat tissue is a considerable source of the stem cells for clinical applications.

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来源期刊
CiteScore
4.20
自引率
0.00%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Investigative Surgery publishes peer-reviewed scientific articles for the advancement of surgery, to the ultimate benefit of patient care and rehabilitation. It is the only journal that encompasses the individual and collaborative efforts of scientists in human and veterinary medicine, dentistry, basic and applied sciences, engineering, and law and ethics. The journal is dedicated to the publication of outstanding articles of interest to the surgical research community.
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