Kim Pauwaert, Samuel Bodé, Upeshka Sewwandi Alwis, Toon Mylle, Pascal Boeckx, Joris Delanghe, Karel Everaert, Saskia Roggeman
{"title":"观察人体内的水处理以解决间质作用:氧化氘和生物阻抗分析可用性的初步结果-一个试点分析。","authors":"Kim Pauwaert, Samuel Bodé, Upeshka Sewwandi Alwis, Toon Mylle, Pascal Boeckx, Joris Delanghe, Karel Everaert, Saskia Roggeman","doi":"10.1080/10256016.2022.2026349","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of this research was to evaluate the relevance of using deuterium oxide (<sup>2</sup>H<sub>2</sub>O) and bio-impedance analysis (BIA) to assess size and function of the interstitium for urological research. Nineteen volunteers were recruited to this prospective trial combining ingestion of <sup>2</sup>H<sub>2</sub>O and BIA. Blood samples were obtained every 10 min after ingestion of <sup>2</sup>H<sub>2</sub>O. Urine was collected before and after the experiment. BIA was performed every 5 min. Body position was alternated to study the effect on fluid distribution. First order kinetics were assumed for the uptake of <sup>2</sup>H<sub>2</sub>O from the gastrointestinal tract to the blood. Sex seemed to have an influence with a significantly slower exchange for women (<i>p</i> = 0.041, men: 0.052 min<sup>-1</sup>, women: 0.038 min<sup>-1</sup>). Impedance measured in legs (men: <i>p</i> = 0.012, women: <i>p</i> = 0.008) and trunk (both <i>p</i> < 0.001) decreased significantly with posture change. These changes probably reflect the orthostatic redistribution of fluid with an increase of fluid in both trunk and legs. Both methods were tested and found to be useful for further urological research. Significant gender differences in <sup>2</sup>H<sub>2</sub>O uptake dynamics from the gastrointestinal pool were observed. An impact of posture changes on the electrical impedance measured was observed.</p>","PeriodicalId":14597,"journal":{"name":"Isotopes in Environmental and Health Studies","volume":"58 1","pages":"99-110"},"PeriodicalIF":1.1000,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Observing the water handling in humans to resolve the role of the interstitium: preliminary results of the usability of deuterium oxide and bio-impedance analysis - a pilot analysis.\",\"authors\":\"Kim Pauwaert, Samuel Bodé, Upeshka Sewwandi Alwis, Toon Mylle, Pascal Boeckx, Joris Delanghe, Karel Everaert, Saskia Roggeman\",\"doi\":\"10.1080/10256016.2022.2026349\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of this research was to evaluate the relevance of using deuterium oxide (<sup>2</sup>H<sub>2</sub>O) and bio-impedance analysis (BIA) to assess size and function of the interstitium for urological research. Nineteen volunteers were recruited to this prospective trial combining ingestion of <sup>2</sup>H<sub>2</sub>O and BIA. Blood samples were obtained every 10 min after ingestion of <sup>2</sup>H<sub>2</sub>O. Urine was collected before and after the experiment. BIA was performed every 5 min. Body position was alternated to study the effect on fluid distribution. First order kinetics were assumed for the uptake of <sup>2</sup>H<sub>2</sub>O from the gastrointestinal tract to the blood. Sex seemed to have an influence with a significantly slower exchange for women (<i>p</i> = 0.041, men: 0.052 min<sup>-1</sup>, women: 0.038 min<sup>-1</sup>). Impedance measured in legs (men: <i>p</i> = 0.012, women: <i>p</i> = 0.008) and trunk (both <i>p</i> < 0.001) decreased significantly with posture change. These changes probably reflect the orthostatic redistribution of fluid with an increase of fluid in both trunk and legs. Both methods were tested and found to be useful for further urological research. Significant gender differences in <sup>2</sup>H<sub>2</sub>O uptake dynamics from the gastrointestinal pool were observed. 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Observing the water handling in humans to resolve the role of the interstitium: preliminary results of the usability of deuterium oxide and bio-impedance analysis - a pilot analysis.
The aim of this research was to evaluate the relevance of using deuterium oxide (2H2O) and bio-impedance analysis (BIA) to assess size and function of the interstitium for urological research. Nineteen volunteers were recruited to this prospective trial combining ingestion of 2H2O and BIA. Blood samples were obtained every 10 min after ingestion of 2H2O. Urine was collected before and after the experiment. BIA was performed every 5 min. Body position was alternated to study the effect on fluid distribution. First order kinetics were assumed for the uptake of 2H2O from the gastrointestinal tract to the blood. Sex seemed to have an influence with a significantly slower exchange for women (p = 0.041, men: 0.052 min-1, women: 0.038 min-1). Impedance measured in legs (men: p = 0.012, women: p = 0.008) and trunk (both p < 0.001) decreased significantly with posture change. These changes probably reflect the orthostatic redistribution of fluid with an increase of fluid in both trunk and legs. Both methods were tested and found to be useful for further urological research. Significant gender differences in 2H2O uptake dynamics from the gastrointestinal pool were observed. An impact of posture changes on the electrical impedance measured was observed.
期刊介绍:
Isotopes in Environmental and Health Studies provides a unique platform for stable isotope studies in geological and life sciences, with emphasis on ecology. The international journal publishes original research papers, review articles, short communications, and book reviews relating to the following topics:
-variations in natural isotope abundance (isotope ecology, isotope biochemistry, isotope hydrology, isotope geology)
-stable isotope tracer techniques to follow the fate of certain substances in soil, water, plants, animals and in the human body
-isotope effects and tracer theory linked with mathematical modelling
-isotope measurement methods and equipment with respect to environmental and health research
-diagnostic stable isotope application in medicine and in health studies
-environmental sources of ionizing radiation and its effects on all living matter