{"title":"预测miR-203a-3p和miR-29a-3p对DNMT3B和GAS7基因表达的可能影响。","authors":"Afgar Ali, Sattarzadeh Bardsiri Mahla, Vahidi Reza, Farsinejad Alireza","doi":"10.1515/jib-2021-0016","DOIUrl":null,"url":null,"abstract":"<p><p>Aberrant expression of genes involved in methylation, including DNA methyltransferase 3 Beta (<i>DNMT3B</i>), can cause hypermethylation of various tumor suppressor genes. In this regard, various molecular factors such as microRNAs can play a critical role in regulating these methyltransferase enzymes and eventually downstream genes such as growth arrest specific 7 (<i>GAS7</i>). Accordingly, in the present study we aimed to predict regulatory effect of miRNAs on <i>DNMT3B</i> and <i>GAS7</i> genes expression in melanoma cell line. hsa-miR-203a-3p and hsa-miR-29a-3p were predicted and selected using bioinformatics software. The Real-time PCR technique was performed to investigate the regulatory effect of these molecules on the <i>DNMT3B</i> and <i>GAS7</i> genes expression. Expression analysis of <i>DNMT3B</i> gene in A375 cell line showed that there was a significant increase compared to control (<i>p</i> value = 0.0015). Analysis of hsa-miR-203a-3p and hsa-miR-29a-3p indicated the insignificant decreased expression in melanoma cell line compared to control (<i>p</i> value < 0.05). Compared to control, the expression of GAS7 gene in melanoma cells showed a significant decrease (<i>p</i> value = 0.0323). Finally, our findings showed that the decreased expression of hsa-miR-203a-3p and hsa-miR-29a-3p can hypothesize that their aberrant expression caused <i>DNMT3B</i> dysfunction, possible methylation of the <i>GAS7</i> gene, and ultimately decreased its expression. However, complementary studies are necessary to definite comment.</p>","PeriodicalId":53625,"journal":{"name":"Journal of Integrative Bioinformatics","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069646/pdf/","citationCount":"3","resultStr":"{\"title\":\"Predicting the possible effect of miR-203a-3p and miR-29a-3p on <i>DNMT3B</i> and <i>GAS7</i> genes expression.\",\"authors\":\"Afgar Ali, Sattarzadeh Bardsiri Mahla, Vahidi Reza, Farsinejad Alireza\",\"doi\":\"10.1515/jib-2021-0016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Aberrant expression of genes involved in methylation, including DNA methyltransferase 3 Beta (<i>DNMT3B</i>), can cause hypermethylation of various tumor suppressor genes. In this regard, various molecular factors such as microRNAs can play a critical role in regulating these methyltransferase enzymes and eventually downstream genes such as growth arrest specific 7 (<i>GAS7</i>). Accordingly, in the present study we aimed to predict regulatory effect of miRNAs on <i>DNMT3B</i> and <i>GAS7</i> genes expression in melanoma cell line. hsa-miR-203a-3p and hsa-miR-29a-3p were predicted and selected using bioinformatics software. The Real-time PCR technique was performed to investigate the regulatory effect of these molecules on the <i>DNMT3B</i> and <i>GAS7</i> genes expression. Expression analysis of <i>DNMT3B</i> gene in A375 cell line showed that there was a significant increase compared to control (<i>p</i> value = 0.0015). Analysis of hsa-miR-203a-3p and hsa-miR-29a-3p indicated the insignificant decreased expression in melanoma cell line compared to control (<i>p</i> value < 0.05). Compared to control, the expression of GAS7 gene in melanoma cells showed a significant decrease (<i>p</i> value = 0.0323). Finally, our findings showed that the decreased expression of hsa-miR-203a-3p and hsa-miR-29a-3p can hypothesize that their aberrant expression caused <i>DNMT3B</i> dysfunction, possible methylation of the <i>GAS7</i> gene, and ultimately decreased its expression. However, complementary studies are necessary to definite comment.</p>\",\"PeriodicalId\":53625,\"journal\":{\"name\":\"Journal of Integrative Bioinformatics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2021-12-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069646/pdf/\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Integrative Bioinformatics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/jib-2021-0016\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MATHEMATICAL & COMPUTATIONAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Bioinformatics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/jib-2021-0016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MATHEMATICAL & COMPUTATIONAL BIOLOGY","Score":null,"Total":0}
Predicting the possible effect of miR-203a-3p and miR-29a-3p on DNMT3B and GAS7 genes expression.
Aberrant expression of genes involved in methylation, including DNA methyltransferase 3 Beta (DNMT3B), can cause hypermethylation of various tumor suppressor genes. In this regard, various molecular factors such as microRNAs can play a critical role in regulating these methyltransferase enzymes and eventually downstream genes such as growth arrest specific 7 (GAS7). Accordingly, in the present study we aimed to predict regulatory effect of miRNAs on DNMT3B and GAS7 genes expression in melanoma cell line. hsa-miR-203a-3p and hsa-miR-29a-3p were predicted and selected using bioinformatics software. The Real-time PCR technique was performed to investigate the regulatory effect of these molecules on the DNMT3B and GAS7 genes expression. Expression analysis of DNMT3B gene in A375 cell line showed that there was a significant increase compared to control (p value = 0.0015). Analysis of hsa-miR-203a-3p and hsa-miR-29a-3p indicated the insignificant decreased expression in melanoma cell line compared to control (p value < 0.05). Compared to control, the expression of GAS7 gene in melanoma cells showed a significant decrease (p value = 0.0323). Finally, our findings showed that the decreased expression of hsa-miR-203a-3p and hsa-miR-29a-3p can hypothesize that their aberrant expression caused DNMT3B dysfunction, possible methylation of the GAS7 gene, and ultimately decreased its expression. However, complementary studies are necessary to definite comment.