Nocardioides sp. PD653对六氯苯和五氯酚的好氧脱氯机理

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY
Koji Ito
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引用次数: 1

摘要

我们试图阐明持久性有机污染物六氯苯(HCB)和五氯酚(PCP)的有氧脱氯机制。我们对Nocardioides sp. PD653的脱卤酶基因进行了基因组和异源表达分析,Nocardioides sp. PD653是第一个发现能够在有氧条件下通过PCP矿化HCB的细菌。鉴定并鉴定了hcbA1A2A3和hcbB1B2B3两个分别参与催化HCB和PCP有氧脱氯的基因;它们被归类为双组分黄扩散单加氧酶家族的成员。随后通过生化分析证实了这一点;在黄素、NADH、黄素还原酶HcbA3和HCB单加氧酶HcbA1存在的条件下,体外成功地重建了好氧脱氯活性。这些发现将有助于实施HCB或pcp污染场所的原位生物修复,以及更好地了解细菌在有氧条件下降解重氯化人为化合物的能力的进化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mechanisms of aerobic dechlorination of hexachlorobenzene and pentachlorophenol by <i>Nocardioides</i> sp. PD653.

Mechanisms of aerobic dechlorination of hexachlorobenzene and pentachlorophenol by <i>Nocardioides</i> sp. PD653.

Mechanisms of aerobic dechlorination of hexachlorobenzene and pentachlorophenol by <i>Nocardioides</i> sp. PD653.

Mechanisms of aerobic dechlorination of hexachlorobenzene and pentachlorophenol by Nocardioides sp. PD653.

We sought to elucidate the mechanisms underlying the aerobic dechlorination of the persistent organic pollutants hexachlorobenzene (HCB) and pentachlorophenol (PCP). We performed genomic and heterologous expression analyses of dehalogenase genes in Nocardioides sp. PD653, the first bacterium found to be capable of mineralizing HCB via PCP under aerobic conditions. The hcbA1A2A3 and hcbB1B2B3 genes, which were involved in catalysing the aerobic dechlorination of HCB and PCP, respectively, were identified and characterized; they were classified as members of the two-component flavin-diffusible monooxygenase family. This was subsequently verified by biochemical analysis; aerobic dechlorination activity was successfully reconstituted in vitro in the presence of flavin, NADH, the flavin reductase HcbA3, and the HCB monooxygenase HcbA1. These findings will contribute to the implementation of in situ bioremediation of HCB- or PCP-contaminated sites, as well as to a better understanding of bacterial evolution apropos their ability to degrade heavily chlorinated anthropogenic compounds under aerobic conditions.

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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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