青藤碱对异丙肾上腺素诱导小鼠心肌肥厚的保护作用。

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Journal of applied biomedicine Pub Date : 2021-09-01 Epub Date: 2021-05-27 DOI:10.32725/jab.2021.014
Le Li, Pu Fang, Jiekun Chen, Cailing Zhang, Houquan Tao
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引用次数: 4

摘要

为了研究青藤碱(Sin)对异丙肾上腺素(Iso, β-激动剂)诱导的心肌肥厚(CH)的影响,我们建立了4个小鼠组:对照组、Iso模型、Iso+美托洛尔(Met, β受体阻滞剂)60 mg/kg和Iso+Sin 120 mg/kg。小鼠用Iso (s.c.c)诱导CH 28 d,灌胃给予Sin或Met共28 d。超声心动图测量左室舒张前壁厚度(LVAWd)、左室舒张后壁厚度(LVPWd)、左室射血分数(LVEF)、短轴缩短(FS)。丙二醛(MDA)和总超氧化物歧化酶(T-SOD)检测试剂盒。ELISA检测乳酸脱氢酶(LDH)、肿瘤坏死因子-α (TNF-α)、白细胞介素-1β (IL-1β)水平。苏木精-伊红(HE)染色及Masson染色观察组织学变化。免疫组化法检测核转录因子κB (NF-κB)蛋白水平。与对照组比较,Iso模型组LVAWd、左室重量指数(LVWI)、心肌纤维化显著升高,NF-κB、LDH、MDA、TNF-α、IL-1β水平显著升高。但T-SOD活性降低。与Iso模型组比较,Iso模型+Sin或Iso模型+Met组LVWI升高,LVAWd、LVPWd及心肌纤维化降低,NF-κB、LDH、MDA、TNF-α、IL-1β水平降低。T-SOD活性也增加。本研究发现,Sin抑制NF-κB的活化,降低TNF-α和IL-1β水平,具有抗氧化应激作用,抑制小鼠心脏心肌炎症,从而证明其预防Iso诱导的CH的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effect of sinomenine on isoproterenol-induced cardiac hypertrophy in mice.

To study the effect of sinomenine (Sin) on isoproterenol (Iso, β-agonist)-induced cardiac hypertrophy (CH), we set up four mouse groups: control, Iso model, Iso+metoprolol (Met, β blocker) 60 mg/kg and Iso+Sin 120 mg/kg. CH was induced by Iso (s.c. for 28 days) in mice, and Sin or Met were orally administered by gavage for 28 days in total. Left ventricular diastolic anterior wall thickness (LVAWd), left ventricular diastolic posterior wall thickness (LVPWd), left ventricular ejection fraction (LVEF), and short axis shortening (FS) were measured by echocardiography. Malondialdehyde (MDA) and total superoxide dismutase (T-SOD) were measured by commercial kits. Lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) were measured by ELISA kits. Histological changes were observed using hematoxylin-eosin (HE) and Masson staining. Protein level of nuclear transcription factor-kappa B (NF-κB) was detected by immunohistochemistry. Compared with the control group, LVAWd, Left ventricular weight index (LVWI) and myocardial fibrosis of the Iso model group significantly increased, as well as NF-κB, LDH, MDA, TNF-α, and IL-1β levels. However, the activity of T-SOD decreased. Compared with the Iso model group, LVWI of Iso model+Sin or Iso model+Met group was improved, LVAWd, LVPWd and myocardial fibrosis decreased, and NF-κB, LDH, MDA, TNF-α and IL-1β levels decreased. T-SOD activity also increased. This study reveals that Sin inhibits the activation of NF-κB, lowers the levels of TNF-α and IL-1β, has anti-oxidative stress effect and inhibits myocardial inflammation in mouse heart, thereby demonstrating its efficacy in preventing Iso induced CH.

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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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