荧光感应细胞的生物能量和物理化学状态。

4区 生物学 Q4 Biochemistry, Genetics and Molecular Biology
Current topics in membranes Pub Date : 2021-01-01 Epub Date: 2021-11-02 DOI:10.1016/bs.ctm.2021.10.002
Luca Mantovanelli, Bauke F Gaastra, Bert Poolman
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引用次数: 2

摘要

荧光传感器在生物学研究中起着重要作用。这些传感器可以基于荧光蛋白、荧光探针,也可以是混合系统。大量荧光分子数据集的可用性,包括遗传编码和合成,以及对许多传感领域的结构见解,允许合理设计各种各样的传感器,能够监测活细胞或体外系统中的分子和全局变化。荧光成像领域的进步帮助研究人员通过将荧光传感器的读数与荧光显微镜技术提供的空间信息相结合,更深入地了解细胞或体外特定变化的发生方式和位置。在这篇综述中,我们概述了荧光生物传感器和荧光成像技术领域的最新进展,并最终指导读者选择荧光工具和技术的最佳组合来回答特定的生物学问题。我们特别关注用于探测细胞的生物能量学和物理化学状态的传感器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fluorescence-based sensing of the bioenergetic and physicochemical status of the cell.

Fluorescence-based sensors play a fundamental role in biological research. These sensors can be based on fluorescent proteins, fluorescent probes or they can be hybrid systems. The availability of a very large dataset of fluorescent molecules, both genetically encoded and synthetically produced, together with the structural insights on many sensing domains, allowed to rationally design a high variety of sensors, capable of monitoring both molecular and global changes in living cells or in in vitro systems. The advancements in the fluorescence-imaging field helped researchers to obtain a deeper understanding of how and where specific changes occur in a cell or in vitro by combining the readout of the fluorescent sensors with the spatial information provided by fluorescent microscopy techniques. In this review we give an overview of the state of the art in the field of fluorescent biosensors and fluorescence imaging techniques, and eventually guide the reader through the choice of the best combination of fluorescent tools and techniques to answer specific biological questions. We particularly focus on sensors for probing the bioenergetics and physicochemical status of the cell.

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来源期刊
Current topics in membranes
Current topics in membranes 生物-生化与分子生物学
CiteScore
3.50
自引率
0.00%
发文量
10
审稿时长
>12 weeks
期刊介绍: Current Topics in Membranes provides a systematic, comprehensive, and rigorous approach to specific topics relevant to the study of cellular membranes. Each volume is a guest edited compendium of membrane biology.
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