可育和不育男性精液样品中大肠杆菌不同o抗原血清群的比较。

Ali Nabi, Mohammad Bagher Khalili, Gilda Eslami, Mahmood Vakili, Fatemeh Anbari, Alireza Torki
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引用次数: 0

摘要

目的:男性生殖道感染与不育症有关,大肠杆菌作为一种重要的细菌已引起越来越多的关注。本研究旨在描述和比较大肠杆菌o抗原在可育和不育男性精液样本中的分布。方法:在本病例对照研究中,收集618名可育男性和1535名不育男性的精液样本。根据世界卫生组织2010年指南,对大肠杆菌阳性样本的浓度、形态、活力和运动性参数进行评估。最后,利用多重聚合酶链反应对大肠杆菌的o抗原变异进行鉴定。结果:育龄男性大肠杆菌感染率明显高于不育男性(p结论:尽管育龄男性大肠杆菌感染率较高,但大肠杆菌对不育男性精液参数的影响更大。可育组和不育组的大肠杆菌血清群无显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A comparison of different O-antigen serogroups of Escherichia coli in semen samples of fertile and infertile men.

A comparison of different O-antigen serogroups of Escherichia coli in semen samples of fertile and infertile men.

Objective: Male genital tract infections have been associated with infertility, and Escherichia coli has drawn increasing attention as an important bacterium in this context. This investigation aimed to characterize and compare the distributions of O-antigen serogroups of E. coli in the semen samples of fertile and infertile men.

Methods: In this case-control study, semen samples were collected from 618 fertile and 1,535 infertile men. The E. coli-positive samples were evaluated in terms of concentration, morphology, viability, and motility parameters according to the World Health Organization 2010 guidelines. Finally, different serogroups of E. coli were identified by multiplex polymerase chain reaction targeting the O-antigen variations of the bacterium.

Results: The prevalence of E. coli among fertile men was significantly higher than among infertile men (p<0.001). The sperm morphology, viability, and motility in the E. coli-positive fertile group were significantly higher than in the E. coli-positive infertile group (p<0.001). E. coli O6 was the most prevalent serogroup found in both groups. However, there was no significant difference in the frequency of different serogroups of E. coil between the two groups (p=0.55).

Conclusion: Despite the higher prevalence of E. coli among fertile men, E. coli had more detrimental effects on semen parameters in infertile men. There was no significant difference in E. coli serogroups between the fertile and infertile groups.

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