辣木叶80%氢甲醇提取物对小鼠的止泻和抗分泌作用。

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
Biochemistry Research International Pub Date : 2022-01-30 eCollection Date: 2022-01-01 DOI:10.1155/2022/5768805
Menbere Getaneh Woldeyohannes, Gelila Tamyalew Eshete, Alfoalem Araba Abiye, Abebe Ejigu Hailu, Solomon Assefa Huluka, Wondmagegn Tamiru Tadesse
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引用次数: 5

摘要

简介:在埃塞俄比亚,不同的药用植物已被声称和用于治疗腹泻疾病。然而,这些声称对大多数药用植物的作用尚未得到科学证实。在埃塞俄比亚民间医学中,这种植物之一是辣木(Moringa stenopetala),在埃塞俄比亚南部通常被当作蔬菜食用。因此,本研究旨在评价辣木叶80%甲醇提取物在不同小鼠模型上的止泻和抗分泌作用。方法:采用蓖麻油致腹泻模型、炭粉胃肠运动模型和蓖麻油致分泌模型对瑞士白化小鼠的止泻作用进行评价。在所有的试验模型中,动物被随机分为五组,每组6只动物。ⅰ组给药0.5 ml(2% ~ 80),ⅱ组给药标准药物(洛哌丁胺3 mg/kg),ⅲ~ V组给药辣木叶甲醇提取物150、300、450 mg/kg。记录大便(腹泻)的发病、次数、稠度和重量,并计算不同参数和百分比比例。结果:在蓖麻油致腹泻模型中,150mg /kg、300mg /kg和450mg /kg蓖麻油提取物对腹泻的抑制率分别为48.5、58.6%和60%。与阴性对照组相比,炭粉提取物在以炭粉为基础的胃肠运动测试中显示出36.8%、54.4和55.9%的蠕动减少。此外,在抗分泌试验中,150、300和450 mg/kg剂量的MEMS对粪便液体含量的抑制作用分别比载体处理组高11.5%、54.54%和61.82%。结论:辣木叶80%甲醇提取物具有止泻作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of <i>Moringa stenopetala</i> Baker f. in Mice.

Antidiarrheal and Antisecretory Effect of 80% Hydromethanolic Leaf Extract of Moringa stenopetala Baker f. in Mice.

Introduction: In Ethiopia, different medicinal plants have been claimed and used to treat diarrheal diseases. However, these claimed effects for most medicinal plants have not been scientifically verified. One of such plants in Ethiopian folkloric medicine is Moringa stenopetala, which is usually consumed as a vegetable in southern Ethiopia. Thus, this study aimed to evaluate the antidiarrheal and antisecretory effects of 80% methanolic leaf extract of Moringa stenopetala in different mice models.

Method: Using Swiss albino mice, castor oil-induced diarrhea, charcoal meal-based gastrointestinal motility, and castor oil-induced secretion models were employed to assess antidiarrheal activity. In all of the test models, animals were randomly assigned into five groups consisting of six animals in each group. Group I received 0.5 ml of the vehicle (2% tween-80), while group II was treated with standard drug (3 mg/kg loperamide) in the respective models, whereas groups III to V received 150, 300, and 450 mg/kg of the methanolic leaf extracts of Moringa stenopetala. Onset, frequency, consistency, and weight of stool (diarrhea) were recorded, and different parameters and percentage proportions were calculated. Data were analyzed using one-way ANOVA followed by Tukey's test, and p < 0.05 was considered statistically significant at 95% confidence of interval.

Result: In the castor oil-induced diarrheal model, the percentage inhibition of diarrhea was 48.5, 58.6, and 60% for the respective doses of 150, 300, and 450 mg/kg of the extract. And, the extract showed a 36.8, 54.4, and 55.9% reduction of peristalsis in charcoal meal-based gastrointestinal motility test compared to the negative control group. Moreover, in the antisecretory assay, the 150, 300, and 450 mg/kg doses of MEMS inhibited fluid contents of the stool by 11.5, 54.54, and 61.82%, respectively, relative to the vehicle-treated group.

Conclusion: The findings revealed that the 80% methanolic leaf extract of Moringa stenopetala extract has shown antidiarrheal activity.

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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
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