Gary Tse , Guoliang Hao , Sharen Lee , Jiandong Zhou , Qingpeng Zhang , Yimei Du , Tong Liu , Shuk Han Cheng , Wing Tak Wong
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Standard deviation (SD) of APDs was 0.3 ± 0.2 ms, coefficient of variation was 0.9 ± 0.8% and the root mean square (RMS) of successive differences in APDs was 0.15 ± 0.14 ms. Poincaré plots of APD<sub>n+1</sub> against APD<sub>n</sub> revealed ellipsoid morphologies with a SD along the line-of-identity (SD2) to SD perpendicular to the line-of-identity (SD1) ratio of 4.6 ± 2.1. Approximate and sample entropy were 0.61 ± 0.12 and 0.76 ± 0.26, respectively. Detrended fluctuation analysis revealed short- and long-term fluctuation slopes of 1.49 ± 0.27 and 0.81 ± 0.36, respectively. Heptanol at 2 mM induced ventricular tachycardia in five out of six hearts. None of the above parameters were altered by heptanol during which reproducible electrical activity was observed (KW-ANOVA, <em>P</em> > 0.05). Contrastingly, SD2/SD1 decreased to 2.03 ± 0.41, approximate and sample entropy increased to 0.82 ± 0.12 and 1.45 ± 0.34, and short-term fluctuation slope decreased to 0.82 ± 0.19 during the 20-s period preceding spontaneous ventricular tachy-arrhythmias (n = 6, KW-ANOVA, P < 0.05).</p></div><div><h3>Conclusion</h3><p>Measures of repolarization variability, such as SD2/SD1, entropy, and fluctuation slope are altered preceding the occurrence of ventricular arrhythmogenesis in mouse hearts. Changes in these variables may allow detection of impending arrhythmias for early intervention.</p></div>","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.crphys.2021.04.001","citationCount":"4","resultStr":"{\"title\":\"Measures of repolarization variability predict ventricular arrhythmogenesis in heptanol-treated Langendorff-perfused mouse hearts\",\"authors\":\"Gary Tse , Guoliang Hao , Sharen Lee , Jiandong Zhou , Qingpeng Zhang , Yimei Du , Tong Liu , Shuk Han Cheng , Wing Tak Wong\",\"doi\":\"10.1016/j.crphys.2021.04.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Time-domain and non-linear methods can be used to quantify beat-to-beat repolarization variability but whether measures of repolarization variability can predict ventricular arrhythmogenesis in mice have never been explored.</p></div><div><h3>Methods</h3><p>Left ventricular monophasic action potentials (MAPs) were recorded during constant right ventricular 8 Hz pacing in Langendorff-perfused mouse hearts, in the presence or absence of the gap junction and sodium channel inhibitor heptanol (0.1, 0.5, 1 or 2 mM).</p></div><div><h3>Results</h3><p>Under control conditions, mean action potential duration (APD) was 39.4 ± 8.1 ms. Standard deviation (SD) of APDs was 0.3 ± 0.2 ms, coefficient of variation was 0.9 ± 0.8% and the root mean square (RMS) of successive differences in APDs was 0.15 ± 0.14 ms. Poincaré plots of APD<sub>n+1</sub> against APD<sub>n</sub> revealed ellipsoid morphologies with a SD along the line-of-identity (SD2) to SD perpendicular to the line-of-identity (SD1) ratio of 4.6 ± 2.1. Approximate and sample entropy were 0.61 ± 0.12 and 0.76 ± 0.26, respectively. Detrended fluctuation analysis revealed short- and long-term fluctuation slopes of 1.49 ± 0.27 and 0.81 ± 0.36, respectively. Heptanol at 2 mM induced ventricular tachycardia in five out of six hearts. None of the above parameters were altered by heptanol during which reproducible electrical activity was observed (KW-ANOVA, <em>P</em> > 0.05). Contrastingly, SD2/SD1 decreased to 2.03 ± 0.41, approximate and sample entropy increased to 0.82 ± 0.12 and 1.45 ± 0.34, and short-term fluctuation slope decreased to 0.82 ± 0.19 during the 20-s period preceding spontaneous ventricular tachy-arrhythmias (n = 6, KW-ANOVA, P < 0.05).</p></div><div><h3>Conclusion</h3><p>Measures of repolarization variability, such as SD2/SD1, entropy, and fluctuation slope are altered preceding the occurrence of ventricular arrhythmogenesis in mouse hearts. 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引用次数: 4
摘要
时域和非线性方法可用于量化搏动复极变异性，但复极变异性的测量是否可以预测小鼠室性心律失常的发生从未被探索过。方法记录langendorff灌注小鼠右心室8 Hz恒定起搏时，存在或不存在间隙连接和钠通道抑制剂heptanol(0.1、0.5、1、2 mM)时左室单相动作电位(MAPs)。结果在对照组，动作电位持续时间(APD)平均为39.4±8.1 ms。apd的标准差(SD)为0.3±0.2 ms，变异系数为0.9±0.8%，连续差异的均方根(RMS)为0.15±0.14 ms。APDn+1与APDn的poincarcars图显示为椭球形态，沿同源线(SD2)的SD与垂直同源线(SD1)的SD之比为4.6±2.1。近似熵为0.61±0.12，样本熵为0.76±0.26。无趋势波动分析显示，短期和长期波动斜率分别为1.49±0.27和0.81±0.36。2毫米Heptanol诱导6颗心脏中的5颗室性心动过速。在观察到可重现的电活动期间，庚醇没有改变上述参数(KW-ANOVA, P >0.05)。自发性室性心律失常发生前20 s内，SD2/SD1下降至2.03±0.41，近似熵和样本熵分别上升至0.82±0.12和1.45±0.34，短期波动斜率下降至0.82±0.19 (n = 6, KW-ANOVA, P <0.05)。结论小鼠心脏复极变异性指标SD2/SD1、熵和波动斜率在室性心律失常发生前发生改变。这些变量的变化可能有助于发现即将发生的心律失常，以便进行早期干预。
Measures of repolarization variability predict ventricular arrhythmogenesis in heptanol-treated Langendorff-perfused mouse hearts
Background
Time-domain and non-linear methods can be used to quantify beat-to-beat repolarization variability but whether measures of repolarization variability can predict ventricular arrhythmogenesis in mice have never been explored.
Methods
Left ventricular monophasic action potentials (MAPs) were recorded during constant right ventricular 8 Hz pacing in Langendorff-perfused mouse hearts, in the presence or absence of the gap junction and sodium channel inhibitor heptanol (0.1, 0.5, 1 or 2 mM).
Results
Under control conditions, mean action potential duration (APD) was 39.4 ± 8.1 ms. Standard deviation (SD) of APDs was 0.3 ± 0.2 ms, coefficient of variation was 0.9 ± 0.8% and the root mean square (RMS) of successive differences in APDs was 0.15 ± 0.14 ms. Poincaré plots of APDn+1 against APDn revealed ellipsoid morphologies with a SD along the line-of-identity (SD2) to SD perpendicular to the line-of-identity (SD1) ratio of 4.6 ± 2.1. Approximate and sample entropy were 0.61 ± 0.12 and 0.76 ± 0.26, respectively. Detrended fluctuation analysis revealed short- and long-term fluctuation slopes of 1.49 ± 0.27 and 0.81 ± 0.36, respectively. Heptanol at 2 mM induced ventricular tachycardia in five out of six hearts. None of the above parameters were altered by heptanol during which reproducible electrical activity was observed (KW-ANOVA, P > 0.05). Contrastingly, SD2/SD1 decreased to 2.03 ± 0.41, approximate and sample entropy increased to 0.82 ± 0.12 and 1.45 ± 0.34, and short-term fluctuation slope decreased to 0.82 ± 0.19 during the 20-s period preceding spontaneous ventricular tachy-arrhythmias (n = 6, KW-ANOVA, P < 0.05).
Conclusion
Measures of repolarization variability, such as SD2/SD1, entropy, and fluctuation slope are altered preceding the occurrence of ventricular arrhythmogenesis in mouse hearts. Changes in these variables may allow detection of impending arrhythmias for early intervention.
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