鹅脂肪肝释放具有潜在全身效应的microrna的筛选。

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Xue Fan, Ya Xing, Long Liu, Chao Zhao, Zhenzhen Chen, Mawahib K Khogali, Minmeng Zhao, Xuming Hu, Hengmi Cui, Tuoyu Geng, Daoqing Gong
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引用次数: 1

摘要

组织器官之间的通讯在维持正常的生理功能和疾病的发生发展中起着重要作用。通讯分子是组织和器官之间相互作用的桥梁,不仅在其分泌的组织和器官中发挥局部作用,而且通过循环对整个身体产生全身作用。本研究通过对过量饲养和正常饲养(对照)朗德鹅的血液microrna组学分析发现,过量饲养的朗德鹅血液中21种microrna (miRNAs)的含量均显著高于对照组。这些mirna可能在鹅脂肪肝的发展过程中具有系统性作用,也可能是鹅脂肪肝诊断的候选标记物。我们使用定量PCR检测了miR-143、miR-455-5p、miR-222a-5p、miR-184、miR-1662和miR-129-5p在鹅脂肪肝中的表达与在正常肝脏中的表达。除miR-129-5p外,这些mirna在鹅脂肪肝中的表达量也显著高于正常肝脏(miR-143的预测靶基因PIGFBP5在鹅脂肪肝中较正常肝脏显著降低)(PmiR-143可能通过抑制IGFBP5的表达而发挥局部和全身作用,从而促进鹅脂肪肝的发展。总之,我们确定了几个mirna,包括我们验证的mirna(即miR-143, miR-455-5p, miR-222a-5p, miR-184, miR-1662和miR-129-5p),它们可以作为鹅脂肪肝诊断的候选标记物,以及促进鹅脂肪肝发展的本地和全球调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Screening of MicroRNAs with Potential Systemic Effects Released from Goose Fatty Liver.

Screening of MicroRNAs with Potential Systemic Effects Released from Goose Fatty Liver.

Screening of MicroRNAs with Potential Systemic Effects Released from Goose Fatty Liver.

Communication between tissues and organs plays an important role in the maintenance of normal physiological functions as well as the occurrence and development of diseases. Communication molecules act as a bridge for interactions between tissues and organs, playing not only a local role in the tissues and organs where they are secreted but also in exerting systemic effects on the whole body via circulation. In this study, blood microRNA-omics analysis of overfed vs. normally fed (control) Landes geese revealed that the content of each of the 21 microRNAs (miRNAs) in the blood of overfed geese was significantly higher than that in the blood of control geese. These miRNAs may have systematic effects in the development of goose fatty liver as well as being candidate markers for the diagnosis of goose fatty liver. We determined the expression of miR-143, miR-455-5p, miR-222a-5p, miR-184, miR-1662, and miR-129-5p using quantitative PCR in goose fatty liver vs. that in normal liver. The expression of these miRNAs, except miR-129-5p, in goose fatty liver was also significantly higher than that in normal liver (P<0.05), suggesting that these blood miRNAs are released from goose fatty liver. In addition, we found that expression of IGFBP5, the predicted target gene of miR-143, was significantly decreased in goose fatty liver vs. the normal liver (P<0.05), indicating that miR-143 may exert both local and systematic effects by inhibiting the expression of IGFBP5, thus promoting the development of goose fatty liver. In conclusion, we identified several miRNAs, including those we validated (i.e., miR-143, miR-455-5p, miR-222a-5p, miR-184, miR-1662, and miR-129-5p) that may serve as candidate markers in the diagnosis of goose fatty liver as well as local and global regulators contributing to the development of goose fatty liver.

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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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