维拉帕米诱导自噬促进非酒精性脂肪肝小鼠肝脏再生。

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM
Jian-Lin Lai, Yuan-E Lian, Jun-Yi Wu, Yao-Dong Wang, Yan-Nan Bai
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引用次数: 6

摘要

维拉帕米可以恢复高脂喂养小鼠的细胞内钙稳态,增加自噬体和溶酶体的融合,减少脂滴积聚,抑制炎症和胰岛素抵抗。本研究旨在研究维拉帕米在非酒精性脂肪肝小鼠中的作用及其潜在的肝脏再生机制。在进行50%肝切除术后,通过检测每个时间点的细胞增殖和自噬来评估自噬和肝脏再生的变化。然后,轻度至中度脂肪肝组和重度脂肪肝组在手术前腹膜内注射25mg/kg维拉帕米10天。对照组和轻度至中度脂肪肝组在术后24-48小时达到增殖高峰,重度脂肪肝和脂肪性肝炎小鼠在48-72小时达到增殖峰值。正常组和轻度至中度脂肪肝组的自噬在术后48小时达到高峰。维拉帕米注射液可以增强小鼠的自噬能力,减轻脂肪肝小鼠的体重,改善肝功能和肝脏再生。维拉帕米可通过mTOR非依赖性信号通路诱导自噬,改善肝细胞功能,促进肝细胞再生,从而改善肝部分切除后的肝再生过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice.

Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice.

Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice.

Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice.

Verapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying liver regeneration mechanism in mice with non-alcoholic fatty liver. After 50% hepatectomy was performed, the changes of autophagy and liver regeneration were evaluated by detecting cell proliferation and autophagy at each time point. Then, 25mg/kg verapamil was injected intraperitoneally for 10 d before an operation in the mild to moderate fatty liver and severe fatty liver groups. The control group and mild to moderate fatty liver group reached the peak of proliferation at 24-48h after operation, and the mice with severe fatty liver and steatohepatitis reached the peak at 48-72h. Autophagy in the normal group and mild to moderate fatty liver group reached the peak 48 hours after operation. Verapamil injection can enhance autophagy, reduce the weight of fatty liver mice, improve liver function and liver regeneration. Verapamil can induce autophagy, improve hepatocyte function and promote hepatocyte regeneration through the mTOR independent signaling pathway, thus improving the process of liver regeneration after partial hepatectomy.

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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
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