定量图像分析评估生物源性氯化银纳米颗粒对胶质母细胞瘤细胞系的抗增殖作用。

IF 3.8 4区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
IET nanobiotechnology Pub Date : 2021-08-01 Epub Date: 2021-03-22 DOI:10.1049/nbt2.12038
Nathalia Müller, Mateus Eugenio, Luciana F Romão, Jorge Marcondes de Souza, Soniza V Alves-Leon, Loraine Campanati, Celso Sant'Anna
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引用次数: 0

摘要

胶质母细胞瘤是中枢神经系统最致命的肿瘤。替莫唑胺(TMZ)是治疗胶质母细胞瘤的首选口服药物,但其疗效较低。银纳米颗粒(AgNPs)已被证明在多种微生物中具有生物杀灭活性,包括一些致病微生物。本文通过对胶质母细胞瘤细胞系(GBM02和GBM11)和星形胶质细胞的自动定量图像分析(HCA)来评估AgCl-NPs对胶质母细胞瘤细胞的抗增殖作用。以0.1 ~ 5.0 μg/ml AgCl-NPs或9.7 ~ 48.5 μg/ml TMZ处理细胞。接受联合治疗的细胞也进行了分析。在AgCl-NPs、GBM02和GBM11的最大测试浓度下,处理72 h后,生长分别下降93%和40%。TMZ处理使GBM02和GBM11细胞的增殖能力分别下降58%和34%。AgCl-NPs与TMZ联合使用具有中等抗增殖作用;最低浓度的抑制作用与TMZ相似,最高浓度的抑制作用与单独使用AgCl-NPs相似。星形胶质细胞增殖无明显变化。作者的研究结果表明,HCA是一种快速可靠的方法,可用于评估纳米颗粒在单细胞水平上的抗增殖作用,AgCl-NPs是治疗胶质母细胞瘤的有希望的药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image-based analysis.

Assessing the antiproliferative effect of biogenic silver chloride nanoparticles on glioblastoma cell lines by quantitative image-based analysis.

Glioblastoma is the most life-threatening tumour of the central nervous system. Temozolomide (TMZ) is the first-choice oral drug for the treatment of glioblastoma, although it shows low efficacy. Silver nanoparticles (AgNPs) have been shown to exhibit biocidal activity in a variety of microorganisms, including some pathogenic microorganisms. Herein, the antiproliferative effect of AgCl-NPs on glioblastoma cell lines (GBM02 and GBM11) and on astrocytes was evaluated through automated quantitative image-based analysis (HCA) of the cells. The cells were treated with 0.1-5.0 μg/ml AgCl-NPs or with 9.7-48.5 μg/ml TMZ. Cells that received combined treatment were also analysed. At a maximum tested concentration of AgCl-NPs, GBM02 and GBM11, the growth decreased by 93% and 40%, respectively, following 72 h of treatment. TMZ treatment decreased the proliferation of GBM02 and GBM11 cells by 58% and 34%, respectively. Combinations of AgCl-NPs and TMZ showed intermediate antiproliferative effects; the lowest concentrations caused an inhibition similar to that obtained with TMZ, and the highest concentrations caused inhibition similar to that obtained with AgCl-NPs alone. No significant changes in astrocyte proliferation were observed. The authors' findings showed that HCA is a fast and reliable approach that can be used to evaluate the antiproliferative effect of the nanoparticles at the single-cell level and that AgCl-NPs are promising agents for glioblastoma treatment.

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来源期刊
IET nanobiotechnology
IET nanobiotechnology 工程技术-纳米科技
CiteScore
6.20
自引率
4.30%
发文量
34
审稿时长
1 months
期刊介绍: Electrical and electronic engineers have a long and illustrious history of contributing new theories and technologies to the biomedical sciences. This includes the cable theory for understanding the transmission of electrical signals in nerve axons and muscle fibres; dielectric techniques that advanced the understanding of cell membrane structures and membrane ion channels; electron and atomic force microscopy for investigating cells at the molecular level. Other engineering disciplines, along with contributions from the biological, chemical, materials and physical sciences, continue to provide groundbreaking contributions to this subject at the molecular and submolecular level. Our subject now extends from single molecule measurements using scanning probe techniques, through to interactions between cells and microstructures, micro- and nano-fluidics, and aspects of lab-on-chip technologies. The primary aim of IET Nanobiotechnology is to provide a vital resource for academic and industrial researchers operating in this exciting cross-disciplinary activity. We can only achieve this by publishing cutting edge research papers and expert review articles from the international engineering and scientific community. To attract such contributions we will exercise a commitment to our authors by ensuring that their manuscripts receive rapid constructive peer opinions and feedback across interdisciplinary boundaries. IET Nanobiotechnology covers all aspects of research and emerging technologies including, but not limited to: Fundamental theories and concepts applied to biomedical-related devices and methods at the micro- and nano-scale (including methods that employ electrokinetic, electrohydrodynamic, and optical trapping techniques) Micromachining and microfabrication tools and techniques applied to the top-down approach to nanobiotechnology Nanomachining and nanofabrication tools and techniques directed towards biomedical and biotechnological applications (e.g. applications of atomic force microscopy, scanning probe microscopy and related tools) Colloid chemistry applied to nanobiotechnology (e.g. cosmetics, suntan lotions, bio-active nanoparticles) Biosynthesis (also known as green synthesis) of nanoparticles; to be considered for publication, research papers in this area must be directed principally towards biomedical research and especially if they encompass in vivo models or proofs of concept. We welcome papers that are application-orientated or offer new concepts of substantial biomedical importance Techniques for probing cell physiology, cell adhesion sites and cell-cell communication Molecular self-assembly, including concepts of supramolecular chemistry, molecular recognition, and DNA nanotechnology Societal issues such as health and the environment Special issues. Call for papers: Smart Nanobiosensors for Next-generation Biomedical Applications - https://digital-library.theiet.org/files/IET_NBT_CFP_SNNBA.pdf Selected extended papers from the International conference of the 19th Asian BioCeramic Symposium - https://digital-library.theiet.org/files/IET_NBT_CFP_ABS.pdf
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