埃塞俄比亚妇女乳腺癌存档组织中高危和低危 HPV DNA 的检测。

IF 1.6 Q4 ONCOLOGY
International Journal of Breast Cancer Pub Date : 2021-10-11 eCollection Date: 2021-01-01 DOI:10.1155/2021/2140151
Endale Gebregzabher, Daniel Seifu, Wondemagegnhu Tigneh, Yonas Bokretsion, Abebe Bekele, Markos Abebe, Gabriella Lillsunde-Larsson, Christina Karlsson, Mats G Karlsson
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引用次数: 0

摘要

背景:人类乳头状瘤病毒(HPV)与宫颈癌、口腔癌、咽喉癌、肛门癌、阴茎癌、外阴癌或阴道癌的发病有关,但却很少被认为是乳腺癌的病因。最近,一些调查发现乳腺癌与病毒感染有关。高危 HPV 类型,主要是 HPV 类型 16、18、31、33、35、39、45、51、52、56、58 和 59,已被确定为人类致癌物。在这项研究中,我们旨在从埃塞俄比亚妇女的存档乳腺肿瘤组织中检测出 19 种高风险和 9 种低风险 HPV:在这项研究中,纳入了来自亚的斯亚贝巴 Tikur Anbassa 专科医院(埃塞俄比亚)的 75 名乳腺癌患者。瑞典奥雷布洛大学医院使用新型 Anyplex™ II HPV28 检测试剂盒进行了 HPV 检测和基因分型。Anyplex™ II PCR 系统可检测 19 种高风险 HPV 类型(16、18、26、31、33、35、39、45、51、52、53、56、58、59、66、68、69、73 和 82)和 9 种低风险 HPV 类型(6、11、40、42、43、44、54、61 和 70)。p16 的 IHC 检测使用自动系统 Dako Autostainer Link 进行:结果:在 75 项有效检测中,有两项检测发现 HPV 阳性(2.7%)。其中一个病例的高危型 HPV16 基因型呈阳性,另一个病例的高危型 HPV39 和低危型 HPV6 均呈阳性。细胞周期蛋白 p16 在高危型 HPV16 阳性病例中高表达,但在 HPV39 阳性病例中没有表达:结论:埃塞俄比亚乳腺癌患者的 HPV 感染率较低,但基于这些观察结果,还不能对 HPV 在埃塞俄比亚乳腺癌患者乳腺癌发生过程中所起的作用做出评论。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women.

Detection of High- and Low-Risk HPV DNA in Archived Breast Carcinoma Tissues from Ethiopian Women.

Background: Human papilloma virus (HPV) is involved in the development of cancer of the cervix, mouth and throat, anus, penis, vulva, or vagina, but it has not been much considered as a cause of breast cancer. Recently, a number of investigations have linked breast cancer to viral infections. High-risk HPV types, predominantly HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59, are established as carcinogens in humans. In this study we aimed to detect 19 high-risk and 9 low-risk HPVs from archived breast tumor tissue among Ethiopian women.

Methods: In this study, 75 breast cancer patients from Tikur Anbassa Specialized Hospital in Addis Ababa (Ethiopia) were included. HPV detection and genotyping were done using the novel Anyplex™ II HPV28 Detection Assay at the Orebro University Hospital, Sweden. The Anyplex™ II PCR System detects 19 high-risk HPV types (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73, and 82) and 9 low-risk HPV types (6, 11, 40, 42, 43, 44, 54, 61, and 70). IHC for p16 was done using an automated system, the Dako Autostainer Link.

Results: Out of the 75 valid tests, two were found to be positive (2.7%) for HPV. One of the cases was positive for the high-risk HPV16 genotype while the other was positive both for the high-risk HPV39 and the low-risk HPV6. The cell cycle protein p16 was highly expressed in the case positive for the high-risk HPV16, but it was not expressed in the case positive for HPV39.

Conclusion: The prevalence of HPV is low in Ethiopian breast cancer patients, but the role played by HPV in breast carcinogenesis among Ethiopian breast cancer patients cannot be commented based on these observations.

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来源期刊
CiteScore
3.40
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: International Journal of Breast Cancer is a peer-reviewed, Open Access journal that provides a forum for scientists, clinicians, and health care professionals working in breast cancer research and management. The journal publishes original research articles, review articles, and clinical studies related to molecular pathology, genomics, genetic predisposition, screening and diagnosis, disease markers, drug sensitivity and resistance, as well as novel therapies, with a specific focus on molecular targeted agents and immune therapies.
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