lncRNA SNHG7的表达增加通过调节miR-214-5p/TWIST1轴促进子痫前期细胞的活力、迁移和侵袭。

IF 1.5 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Hypertension in Pregnancy Pub Date : 2021-08-01 Epub Date: 2021-10-04 DOI:10.1080/10641955.2021.1981372

Yongping Wang, Zhihui Zhang

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引用次数: 0

摘要

目的:本研究旨在阐明lncRNA SNHG7在子痫前期(pre-子痫，PE)中的分子机制。方法:采用qRT-PCR检测PE胎盘组织中SNHG7、miR-214-5p、TWIST1的表达。SNHG7/miR-214-5p/TWIST1轴对PE的调控机制采用MTT、创面愈合、transwell侵袭和western blot检测。结果:PE妊娠时，SNHG7、TWIST1水平降低，miR-214-5p水平升高。miR-214-5p的升高和TWIST1的降低在一定程度上消除了SNHG7上调对JEG-3细胞活力和转移的促进作用。结论:上调的SNHG7通过调节miR-214-5p/TWIST1轴来保护PE。

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Increased expression of lncRNA SNHG7 promotes the cell viability, migration, and invasion in pre-eclampsia via modulating the miR-214-5p/TWIST1 axis.

Purpose: This study is aimed to elucidate the molecular mechanism of lncRNA SNHG7 on pre-eclampsia (PE).

Methods: The expression of SNHG7, miR-214-5p and TWIST1 in PE placental tissues was detected by qRT-PCR. The regulatory mechanism of SNHG7/miR-214-5p/TWIST1 axis on PE was determined using MTT, wound healing, transwell invasion, and western blot assays.

Results: In PE pregnancies, SNHG7 and TWIST1 were decreased, while miR-214-5p was increased.The elevated miR-214-5p and decreased TWIST1 partly eliminated the promoting effects of SNHG7 up-regulation on the viability and metastasis of JEG-3 cells.

Conclusions: Up-regulated SNHG7 protects against PE through modulating the miR-214-5p/TWIST1 axi.

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来源期刊

Hypertension in Pregnancy 医学-妇产科学

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Hypertension in Pregnancy is a refereed journal in the English language which publishes data pertaining to human and animal hypertension during gestation. Contributions concerning physiology of circulatory control, pathophysiology, methodology, therapy or any other material relevant to the relationship between elevated blood pressure and pregnancy are acceptable. Published material includes original articles, clinical trials, solicited and unsolicited reviews, editorials, letters, and other material deemed pertinent by the editors.