轻度创伤性脑损伤有助于迟发性神经炎症的发展。

IF 2.2 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Neuroimmunomodulation Pub Date : 2022-01-01 Epub Date: 2021-09-28 DOI:10.1159/000519011
Arina Ponomarenko, Anna Tyrtyshnaia, Darya Ivashkevich, Igor Manzhulo
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引用次数: 4

摘要

近年来,根据文献报道,轻度创伤性脑损伤(mTBI)的问题变得越来越紧迫。与中度至重度颅脑外伤相比,mTBI发生的人数要多得多。单次或多次mTBI引起的延迟后遗症是一个重大的公共卫生问题。方法:采用体重下降模型观察mTBI的形成。一根重337克、尖端钝、直径3毫米的金属棒被举起至8厘米高,并由杠杆握住。这种创伤是通过降低杠杆和杆子自由落在大鼠的头骨上造成的。在实验动物的大脑皮层,我们分析了小胶质细胞活性水平(iba -1阳性系统)和促炎标志物(il -1 β、il - 6和CD86)的表达。此外,我们还评估了脑样本中内源性大麻素系统受体(cannabinoid receptor type 1 [CB1])的表达水平。结果:实验表明,mTBI增加了(1)由促炎途径(CD86)激活的小胶质细胞(iba-1)的数量;(2)促炎因子il - 1β、il - 6水平;(3) CB1R活性。结论:总体而言,本研究结果表明mTBI诱导了持续的神经炎症反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mild Traumatic Brain Injury Contributes to the Development of Delayed Neuroinflammation.

Introduction: In recent years, according to the literature, the problem of mild traumatic brain injury (mTBI) has become more and more urgent. Compared to moderate to severe craniocerebral trauma, mTBI occurs in a far greater number of people. The delayed sequelae caused by a single mTBI or multiple mTBIs are a significant public health problem.

Methods: A weight-drop model was used for the formation of mTBI. A metal rod weighing 337 g with a blunt tip of 3 mm diameter was uplifted at 8 cm height and held by a lever. The trauma was created by lowering the lever and the rod and free-dropping onto the rat skull. In the cerebral cortex of experimental animals, we analyzed the level of microglial activity (Iba-1-positive system) and the expression of pro-inflammatory markers (IL1β, IL6, and CD86). Also, the expression level of the endocannabinoid system receptor (cannabinoid receptor type 1 [CB1]) was assessed in brain samples.

Results: Experiments have shown that mTBI increases (1) the amount of microglia (iba-1) activated by the pro-inflammatory pathway (CD86); (2) the level of pro-inflammatory cytokines IL1β and IL6; and (3) CB1R activity.

Conclusion: Overall, the results of this study indicate that mTBI induces a sustained neuroinflammatory response.

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来源期刊
Neuroimmunomodulation
Neuroimmunomodulation 医学-免疫学
CiteScore
3.60
自引率
4.20%
发文量
35
审稿时长
>12 weeks
期刊介绍: The rapidly expanding area of research known as neuroimmunomodulation explores the way in which the nervous system interacts with the immune system via neural, hormonal, and paracrine actions. Encompassing both basic and clinical research, ''Neuroimmunomodulation'' reports on all aspects of these interactions. Basic investigations consider all neural and humoral networks from molecular genetics through cell regulation to integrative systems of the body. The journal also aims to clarify the basic mechanisms involved in the pathogenesis of the CNS pathology in AIDS patients and in various neurodegenerative diseases. Although primarily devoted to research articles, timely reviews are published on a regular basis.
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