血清上皮膜蛋白1可作为肝外胆管癌的可行生物标志物。

IF 2.3 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xiang Li, Lang Yan, Hao Xue
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引用次数: 0

摘要

背景:肝外胆管癌是一种起源于胆管上皮的恶性肿瘤,预后不良。上皮膜蛋白1于1995年首次被发现,在多种癌症中作为癌基因或抗肿瘤基因发挥作用。然而,上皮膜蛋白1在肝外胆管癌中的临床作用尚不清楚。方法:利用基因本体、京都百科全书和基因组通路分析对差异表达基因进行鉴定。采用逆转录-定量聚合酶链式反应法和western blot法检测了183例肝外胆管癌患者和61例健康对照者上皮膜蛋白1的表达水平,并进行了比较。同时,通过受者工作特征和Kaplan-Meier分析来衡量ECCA患者EMP1的诊断和预后。最后比较上皮膜蛋白1表达与临床病理指标的关系,进一步验证上皮膜蛋白1在肝外胆管癌中的临床作用。结果:通过分析GSE76297、GSE89749和GSE26566GO的数据,我们发现了1554个下调基因和1065个上调基因。通过基因本体、京都百科和基因组分析,发现细胞外基质组织、细胞外结构组织、胆固醇代谢、白细胞介素-17信号通路、维生素消化吸收等显著富集并参与靶向差异表达基因。肝外胆管癌患者血清中上皮膜蛋白1信使核糖核酸与健康对照组相比明显降低。受试者工作特征分析显示,上皮膜蛋白1信使核糖核酸曲线下面积为0.9281,诊断肝外胆管癌(95% CI = 0.8967 ~ 0.9595)。相关分析显示上皮膜蛋白1表达与淋巴结转移、肿瘤淋巴结转移分期、癌抗原19-9水平、癌胚抗原水平呈负相关。结论:上皮膜蛋白1的异常表达是区分肝外胆管癌患者与健康对照的重要因素,上皮膜蛋白1的低表达提示预后不良。因此,上皮膜蛋白1具有较高的准确性、敏感性和特异性,是一种可行、可靠的肝外胆管癌诊断和预后的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serum epithelial membrane protein 1 serves as a feasible biomarker in extrahepatic cholangiocarcinoma.

Background: Extrahepatic cholangiocarcinoma is a malignancy that originates from bile duct epithelium with an unfavorable prognosis. Epithelial membrane protein 1 was first discovered in 1995, functioning as an oncogene or anti-tumor gene in various cancers. However, the clinical role of epithelial membrane protein 1 extrahepatic cholangiocarcinoma remained unclear.

Methods: Differentially expressed genes were identified using Gene Ontology and the Kyoto Encyclopedia and Genomes pathway analysis. Out of 183 extrahepatic cholangiocarcinoma patients and 61 healthy controls, the expression level of epithelial membrane protein 1 was detected and compared using reverse transcription-quantitative polymerase chain reaction analysis and western blot assay. Meanwhile, the diagnosis and prognosis of EMP1 in ECCA were measured by receiver operating characteristic and Kaplan-Meier analysis. Finally, the relationship between epithelial membrane protein 1 expression and clinicopathological indexes were compared to further verify the clinical role of epithelial membrane protein 1 in extrahepatic cholangiocarcinoma.

Results: After analyzing data from GSE76297, GSE89749, and GSE26566GO, we found 1554 down-regulated and 1065 up-regulated genes. Through Gene Ontology and Kyoto Encyclopedia and Genomes analysis, extracellular matrix organization, extracellular structure organization, cholesterol metabolism, interleukin-17 signaling pathway, and vitamin digestion and absorption were significantly enriched and involved in targeted differentially expresses genes. Epithelial membrane protein 1 messenger ribonucleic acid was notably decreased in serum samples from extrahepatic cholangiocarcinoma patients, compared with that in healthy controls. Receiver operating characteristic analysis revealed that the area under the curve of epithelial membrane protein 1 messenger ribonucleic acid for the diagnosis of extrahepatic cholangiocarcinoma was 0.9281 (95% CI = 0.8967-0.9595). Moreover, the correlation analysis presented that epithelial membrane protein 1 expression was negatively correlated with lymph node metastasis, tumour node metastasis stage, cancer antigen 19-9 level, and carcinoembryonic antigen level.

Conclusion: Aberrant expression of epithelial membrane protein 1 contributed to distinguishing extrahepatic cholangiocarcinoma patients and healthy controls, and a low expression level of epithelial membrane protein 1 indicated an unfavorable prognosis. Hence, epithelial membrane protein 1 was a feasible and credible biomarker for extrahepatic cholangiocarcinoma diagnosis and prognosis, with high accuracy, sensitivity, and specificity.

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来源期刊
International Journal of Biological Markers
International Journal of Biological Markers 医学-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
43
期刊介绍: IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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