hsa_circ_0005721通过上调线性转录本TEP1触发骨肉瘤的增殖、迁移和侵袭。

Q2 Medicine

Journal of Buon Pub Date : 2021-07-01

Meixin Xu, Xingxia Sun, Yang Liu, Liang Chang, Hasi Te Wang, Shuren Wang

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引用次数: 0

摘要

目的:本研究旨在阐明hsa_circ_0005721在骨肉瘤发生发展中的生物学作用及其分子机制。方法:采用实时荧光定量聚合酶链反应(qRT-PCR)技术检测30对骨肉瘤和非肿瘤组织中hsa_circ_0005721的表达水平。通过功能实验研究hsa_circ_0005721对骨肉瘤细胞增殖、转移率和凋亡率的影响。分别通过双荧光素酶报告基因法和挽救性实验分析hsa_circ_0005721的下游靶点及其在骨肉瘤恶性发展中的协同调控机制。结果:hsa_circ_0005721在骨肉瘤组织和细胞系中表达上调。敲低hsa_circ_0005721抑制U-2OS和Saos-2细胞的增殖和转移率，刺激细胞凋亡。hsa_circ_0005721作为ceRNA，通过竞争性结合miR-16-5p上调线性转录本TEP1，从而发挥其调节骨肉瘤发展的生物学功能。结论:本研究首次在骨肉瘤中发现了上调的hsa_circ_0005721，其通过上调线性转录本TEP1触发骨肉瘤的恶性发展。

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hsa_circ_0005721 triggers proliferation, migration and invasion of osteosarcoma by upregulating the linear transcript TEP1.

Purpose: This study aimed to illustrate the biological role of hsa_circ_0005721 in the development of osteosarcoma and the molecular mechanism.

Methods: hsa_circ_0005721 levels in 30 pairs of osteosarcoma and non-tumor tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Functional experiments were conducted to assess the influence of hsa_circ_0005721 on proliferative, metastatic and apoptotic rates of osteosarcoma cells. The downstream target of hsa_circ_0005721 and their co-regulatory mechanism in malignant development of osteosarcoma were analyzed by dual-luciferase reporter assay and rescue experiments, respectively.

Results: hsa_circ_0005721 was upregulated in osteosarcoma tissues and cell lines. Knockdown of hsa_circ_0005721 suppressed proliferative and metastatic rates of U-2OS and Saos-2 cells, and stimulated apoptosis. Serving as a ceRNA, hsa_circ_0005721 upregulated the linear transcript TEP1 by competitively binding miR-16-5p, thus exerting its biological functions in regulating osteosarcoma development.

Conclusions: This study for the first time identified the upregulated hsa_circ_0005721 in osteosarcoma, which triggers the malignant development of osteosarcoma by upregulating the linear transcript TEP1.

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来源期刊

Journal of Buon 医学-肿瘤学

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： JBUON aims at the rapid diffusion of scientific knowledge in Oncology. Its character is multidisciplinary, therefore all aspects of oncologic activities are welcome including clinical research (medical oncology, radiation oncology, surgical oncology, nursing oncology, psycho-oncology, supportive care), as well as clinically-oriented basic and laboratory research, cancer epidemiology and social and ethical aspects of cancer. Experts of all these disciplines are included in the Editorial Board. With a rapidly increasing body of new discoveries in clinical therapeutics, the molecular mechanisms that contribute to carcinogenesis, advancements in accurate and early diagnosis etc, JBUON offers a free forum for clinicians and basic researchers to make known promptly their achievements around the world. With this aim JBUON accepts a broad spectrum of articles such as editorials, original articles, reviews, special articles, short communications, commentaries, letters to the editor and correspondence among authors and readers. JBUON keeps the characteristics of its former paper print edition and appears as a bimonthly e-published journal with continuous volume, issue and page numbers.