口腔癌前和癌前患者中连接蛋白表达改变作为潜在生物标志物。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
ACS Applied Electronic Materials Pub Date : 2022-04-03 Epub Date: 2021-09-17 DOI:10.1080/21688370.2021.1973329
Puja Upadhaya, Sarbani Giri, Dharmeswar Barhoi, Abhinandan Bhattacharjee
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引用次数: 1

摘要

由于口腔癌晚期患者的生存率较低,发现一种能够诊断和预测疾病进展的生物标志物至关重要。细胞-细胞连接蛋白在组织结构的维持中起着至关重要的作用,但在不同的癌症中往往不受调节。本研究研究了细胞-细胞连接蛋白,即e-钙粘蛋白(E-cad)和小带闭塞-1 (ZO-1)在口腔癌前(OED)和癌前(OSCC)患者中的表达,以监测它们是否可以作为可行的分子标志物。透射电镜观察连接复合物超微结构,免疫组织化学检测蛋白表达。采用Pearson相关检验检测蛋白表达与患者临床病理特征的关系。采用Kaplan-Meier法估计随访资料的生存曲线。我们观察到,与邻近的非癌组织相比,OED和OSCC中的连接复合物被破坏,E-cad和ZO-1的免疫表达显著降低。ZO-1的表达与TNM分期有关,而E-cad与组织学分级和TNM分期有关。ZO-1和E-cad蛋白在OED和OSCC中的表达呈正相关。此外,随访研究显示,高ZO-1和E-cad表达的患者比低表达的患者存活时间更长。目前的研究表明,连接复合体的破坏和连接蛋白表达的改变可能引起卫生专业人员的注意,以探索连接蛋白作为口腔癌可能的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Altered expression of junctional proteins as a potential biomarker in oral precancerous and cancerous patients.

Altered expression of junctional proteins as a potential biomarker in oral precancerous and cancerous patients.

Altered expression of junctional proteins as a potential biomarker in oral precancerous and cancerous patients.

Due to a lower survival rate in patients with advanced clinical stages of oral cancer, discovering a biomarker that could diagnose and predict disease progression is vital. Cell-cell junctional proteins play a crucial role in the maintenance of tissue architecture but are often deregulated in different cancer. The present study investigates the expression of cell-cell junctional proteins viz: e-cadherin (E-cad) and zonula occludens-1 (ZO-1) in oral precancerous (OED) and cancerous (OSCC) patients to monitor if they can serve as practicable molecular markers. The ultrastructural junctional complex was studied by transmission electron microscopy, and the expression of proteins was performed by immunohistochemistry. The relationship between the expression of protein and clinicopathological features of the patients was checked by Pearson's correlation test. Furthermore, the survival curve of the follow-up data was estimated by the Kaplan-Meier method. We observed a disrupted junctional complex and a significantly decreased immunoexpression of E-cad and ZO-1 in OED and OSCC when compared to the adjacent non-cancerous tissues. The expression of ZO-1 was associated with TNM stages, whereas E-cad was associated with histological grades as well as TNM stages. A positive correlation was observed between the expression of ZO-1 and E-cad proteins in OED and OSCC. Further, follow-up studies revealed that high ZO-1 and E-cad expressing patients survived longer than their low expressed counterparts. The present study shows disruption of junctional complex and alteration of junctional proteins expression that could draw the attention of health professionals to explore junctional proteins as a possible therapeutic target in oral cancer.

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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊介绍: ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/​Abstracted: Web of Science SCIE Scopus CAS INSPEC Portico
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