Syncollin是一种抗菌多肽

IF 2.6 2区 生物学 Q3 CELL BIOLOGY
Rosie A. Waters, James Robinson, J. Michael Edwardson
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引用次数: 1

摘要

Syncollin是一种16 kda的蛋白,主要存在于胰腺腺泡细胞的酶原颗粒中,在肠上皮细胞和中性粒细胞中表达水平较低。在这里,我们使用从瞬时转染的哺乳动物细胞的上清中分离的带有strep标记的syncollin来验证syncollin具有抗菌特性的假设,这可能使其在肠道和其他地方的宿主防御中发挥作用。我们发现syncollin是一种非常耐热的蛋白质,其圆二色光谱与主要的β -片结构一致。Syncollin与细菌肽聚糖结合,限制革兰氏阳性(乳酸乳球菌)和革兰氏阴性(大肠杆菌)细菌的生长。Syncollin诱导碘化丙啶进入大肠杆菌(但不是乳杆菌),表明细菌膜的通透性。通过扫描电子显微镜可以看到,它还会导致乳杆菌和大肠杆菌的表面结构损伤。我们认为syncollin是控制肠道微生物组的一大群抗菌多肽中先前未被发现的成员。Take away Syncollin是一种16 kda的蛋白质,存在于胰腺酶原颗粒中。Syncollin具有高度的热稳定性,并具有主要的β -片结构。Syncollin结合肽聚糖,抑制乳杆菌和大肠杆菌的生长。Syncollin导致碘化丙啶进入大肠杆菌(但不是乳杆菌)。Syncollin引起乳杆菌和大肠杆菌的表面结构损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Syncollin is an antibacterial polypeptide

Syncollin is an antibacterial polypeptide

Syncollin is a 16-kDa protein found predominantly in the zymogen granules of pancreatic acinar cells, with expression at lower levels in intestinal epithelial cells and neutrophils. Here, we used Strep-tagged syncollin isolated from the supernatant of transiently transfected mammalian cells to test the hypothesis that syncollin has antibacterial properties, which might enable it to play a role in host defence in the gut and possibly elsewhere. We show that syncollin is an exceptionally thermostable protein with a circular dichroism spectrum consistent with a predominantly beta-sheet structure. Syncollin binds to bacterial peptidoglycan and restricts the growth of representative Gram-positive (Lactococcus lactis) and Gram-negative (Escherichia coli) bacteria. Syncollin induces propidium iodide uptake into E. coli (but not L. lactis), indicating permeabilisation of the bacterial membrane. It also causes surface structural damage in both L. lactis and E. coli, as visualised by scanning electron microscopy. We propose that syncollin is a previously unidentified member of a large group of antimicrobial polypeptides that control the gut microbiome.

Take Aways

  • Syncollin is a 16-kDa protein found in pancreatic zymogen granules.
  • Syncollin is highly thermostable and has a predominantly beta-sheet structure.
  • Syncollin binds peptidoglycan and restricts the growth of L. lactis and E. coli.
  • Syncollin causes propidium iodide uptake into E. coli (but not L. lactis).
  • Syncollin causes surface structural damage in both L. lactis and E. coli.
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来源期刊
Cellular Microbiology
Cellular Microbiology 生物-微生物学
CiteScore
9.70
自引率
0.00%
发文量
26
审稿时长
3 months
期刊介绍: Cellular Microbiology aims to publish outstanding contributions to the understanding of interactions between microbes, prokaryotes and eukaryotes, and their host in the context of pathogenic or mutualistic relationships, including co-infections and microbiota. We welcome studies on single cells, animals and plants, and encourage the use of model hosts and organoid cultures. Submission on cell and molecular biological aspects of microbes, such as their intracellular organization or the establishment and maintenance of their architecture in relation to virulence and pathogenicity are also encouraged. Contributions must provide mechanistic insights supported by quantitative data obtained through imaging, cellular, biochemical, structural or genetic approaches.
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