Celiwe A Ngcamphalala, Michelle Bouwer, Susan W Nicolson, André Ganswindt, Andrew E McKechnie
{"title":"圈养环境下的实验温度似乎不适合评估野生捕获鸟类对热暴露的粪便糖皮质激素代谢物反应。","authors":"Celiwe A Ngcamphalala, Michelle Bouwer, Susan W Nicolson, André Ganswindt, Andrew E McKechnie","doi":"10.1086/716043","DOIUrl":null,"url":null,"abstract":"<p><p>AbstractNoninvasive measurement of stress-related alterations in fecal glucocorticoid metabolite (fGCM) concentrations has considerable potential for quantifying physiological responses to very hot weather in free-ranging birds, but practical considerations related to sampling will often make this method feasible only for habituated study populations. Here we evaluate an alternate approach, the use of experimentally manipulated thermal environments for evaluating stress responses to high environmental temperatures in wild-caught birds housed in captivity. Using an enzyme immunoassay utilizing antibodies against 5ß-pregnane-3α,11ß,21-triol-20-one-CMO∶BSA (tetrahydrocorticosterone), we quantified fGCMs in captive individuals of three southern African arid-zone species (southern pied babblers [<i>Turdoides bicolor</i>], white-browed sparrow-weavers [<i>Plocepasser mahali</i>], and southern yellow-billed hornbills [<i>Tockus leucomelas</i>]) experiencing daily air temperature maxima (<i>T</i><sub>max</sub>) ranging from 30°-32°C to 42°-44°C. For none of the three species did <i>T</i><sub>max</sub> emerge as a significant predictor of elevated fGCM concentrations, and no stress response to simulated hot weather was evident. The apparent lack of a stress response to <i>T</i><sub>max</sub> = 42°C in captive southern pied babblers contrasts with linear increases in fGCMs at <i>T</i><sub>max</sub> > 38°C in free-ranging conspecifics. The lack of an effect of <i>T</i><sub>max</sub> on fGCM levels may potentially be explained by several factors, including differences in operative temperatures and the availability of water and food between free-ranging and captive settings or the stress effect of captivity itself. Our results suggest that experimental manipulations of thermal environments experienced by wild-caught captive birds have limited usefulness for testing hypotheses concerning the effects of hot weather events on fGCM (and, by extension, glucocorticoid) concentrations.</p>","PeriodicalId":54609,"journal":{"name":"Physiological and Biochemical Zoology","volume":"94 5","pages":"330-337"},"PeriodicalIF":1.8000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1086/716043","citationCount":"1","resultStr":"{\"title\":\"Experimental Manipulation of Air Temperature in Captivity Appears Unsuitable for Evaluating Fecal Glucocorticoid Metabolite Responses of Wild-Caught Birds to Heat Exposure.\",\"authors\":\"Celiwe A Ngcamphalala, Michelle Bouwer, Susan W Nicolson, André Ganswindt, Andrew E McKechnie\",\"doi\":\"10.1086/716043\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>AbstractNoninvasive measurement of stress-related alterations in fecal glucocorticoid metabolite (fGCM) concentrations has considerable potential for quantifying physiological responses to very hot weather in free-ranging birds, but practical considerations related to sampling will often make this method feasible only for habituated study populations. Here we evaluate an alternate approach, the use of experimentally manipulated thermal environments for evaluating stress responses to high environmental temperatures in wild-caught birds housed in captivity. Using an enzyme immunoassay utilizing antibodies against 5ß-pregnane-3α,11ß,21-triol-20-one-CMO∶BSA (tetrahydrocorticosterone), we quantified fGCMs in captive individuals of three southern African arid-zone species (southern pied babblers [<i>Turdoides bicolor</i>], white-browed sparrow-weavers [<i>Plocepasser mahali</i>], and southern yellow-billed hornbills [<i>Tockus leucomelas</i>]) experiencing daily air temperature maxima (<i>T</i><sub>max</sub>) ranging from 30°-32°C to 42°-44°C. For none of the three species did <i>T</i><sub>max</sub> emerge as a significant predictor of elevated fGCM concentrations, and no stress response to simulated hot weather was evident. The apparent lack of a stress response to <i>T</i><sub>max</sub> = 42°C in captive southern pied babblers contrasts with linear increases in fGCMs at <i>T</i><sub>max</sub> > 38°C in free-ranging conspecifics. The lack of an effect of <i>T</i><sub>max</sub> on fGCM levels may potentially be explained by several factors, including differences in operative temperatures and the availability of water and food between free-ranging and captive settings or the stress effect of captivity itself. Our results suggest that experimental manipulations of thermal environments experienced by wild-caught captive birds have limited usefulness for testing hypotheses concerning the effects of hot weather events on fGCM (and, by extension, glucocorticoid) concentrations.</p>\",\"PeriodicalId\":54609,\"journal\":{\"name\":\"Physiological and Biochemical Zoology\",\"volume\":\"94 5\",\"pages\":\"330-337\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1086/716043\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Physiological and Biochemical Zoology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1086/716043\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PHYSIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiological and Biochemical Zoology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1086/716043","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
Experimental Manipulation of Air Temperature in Captivity Appears Unsuitable for Evaluating Fecal Glucocorticoid Metabolite Responses of Wild-Caught Birds to Heat Exposure.
AbstractNoninvasive measurement of stress-related alterations in fecal glucocorticoid metabolite (fGCM) concentrations has considerable potential for quantifying physiological responses to very hot weather in free-ranging birds, but practical considerations related to sampling will often make this method feasible only for habituated study populations. Here we evaluate an alternate approach, the use of experimentally manipulated thermal environments for evaluating stress responses to high environmental temperatures in wild-caught birds housed in captivity. Using an enzyme immunoassay utilizing antibodies against 5ß-pregnane-3α,11ß,21-triol-20-one-CMO∶BSA (tetrahydrocorticosterone), we quantified fGCMs in captive individuals of three southern African arid-zone species (southern pied babblers [Turdoides bicolor], white-browed sparrow-weavers [Plocepasser mahali], and southern yellow-billed hornbills [Tockus leucomelas]) experiencing daily air temperature maxima (Tmax) ranging from 30°-32°C to 42°-44°C. For none of the three species did Tmax emerge as a significant predictor of elevated fGCM concentrations, and no stress response to simulated hot weather was evident. The apparent lack of a stress response to Tmax = 42°C in captive southern pied babblers contrasts with linear increases in fGCMs at Tmax > 38°C in free-ranging conspecifics. The lack of an effect of Tmax on fGCM levels may potentially be explained by several factors, including differences in operative temperatures and the availability of water and food between free-ranging and captive settings or the stress effect of captivity itself. Our results suggest that experimental manipulations of thermal environments experienced by wild-caught captive birds have limited usefulness for testing hypotheses concerning the effects of hot weather events on fGCM (and, by extension, glucocorticoid) concentrations.
期刊介绍：
Physiological and Biochemical Zoology: Ecological and Evolutionary Approaches primarily publishes original research in animal physiology and biochemistry as considered from behavioral, ecological, and/or evolutionary perspectives. Studies at all levels of biological organization from the molecular to the whole organism are welcome, and work that integrates across levels of organization is particularly encouraged. Studies that focus on behavior or morphology are welcome, so long as they include ties to physiology or biochemistry, in addition to having an ecological or evolutionary context.
Subdisciplines of interest include nutrition and digestion, salt and water balance, epithelial and membrane transport, gas exchange and transport, acid-base balance, temperature adaptation, energetics, structure and function of macromolecules, chemical coordination and signal transduction, nitrogen metabolism and excretion, locomotion and muscle function, biomechanics, circulation, behavioral, comparative and mechanistic endocrinology, sensory physiology, neural coordination, and ecotoxicology ecoimmunology.
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