苯胺蓝染料磷离子液体的可调细胞毒性和选择性。

Samantha Macchi, Mohd Zubair, Nawab Ali, Grégory Guisbiers, Noureen Siraj
{"title":"苯胺蓝染料磷离子液体的可调细胞毒性和选择性。","authors":"Samantha Macchi,&nbsp;Mohd Zubair,&nbsp;Nawab Ali,&nbsp;Grégory Guisbiers,&nbsp;Noureen Siraj","doi":"10.1166/jnn.2021.19535","DOIUrl":null,"url":null,"abstract":"<p><p>Ionic liquids are an interesting class of materials that have recently been utilized as chemotherapeutic agents in cancer therapy. Aniline blue, a commonly used biological staining agent, was used as a counter ion to trihexyltetradecylphosphonium, a known cytotoxic cation. A facile, single step ion exchange reaction was performed to synthesize a fluorescent ionic liquid, trihexyltetradecylphosphonium aniline blue. Aqueous nanoparticles of this hydrophobic ionic liquid were prepared using reprecipitationmethod. The newly synthesized ionic liquid and subsequent nanoparticles were characterized using various spectroscopic techniques. Transmission electron microscopy and zeta potential measurements were performed to characterize the nanoparticles' morphology and surface charge. The photophysical properties of the nanoparticles and the parent aniline blue compound were studied using absorption and fluorescence spectroscopy. Cell viability studies were conducted to investigate the cytotoxicity of the newly developed trihexyltetradecylphosphonium aniline blue nanoparticles in human breast epithelial cancer cell line (MCF-7) and its corresponding normal epithelial cell line (MCF-10A) <i>in vitro</i>. The results revealed that the synthesized ionic nanomedicines were more cytotoxic (lower IC<sub>50</sub>) than the parent chemotherapeutic compound in MCF-7 cells. Nanoparticles of the synthesized ionic liquid were also shown to be more stable in both aqueous and cellular media and more selective than parent compounds towards cancer cells.</p>","PeriodicalId":16417,"journal":{"name":"Journal of nanoscience and nanotechnology","volume":"21 12","pages":"6143-6150"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919709/pdf/nihms-1783303.pdf","citationCount":"5","resultStr":"{\"title\":\"Tunable Cytotoxicity and Selectivity of Phosphonium Ionic Liquid with Aniline Blue Dye.\",\"authors\":\"Samantha Macchi,&nbsp;Mohd Zubair,&nbsp;Nawab Ali,&nbsp;Grégory Guisbiers,&nbsp;Noureen Siraj\",\"doi\":\"10.1166/jnn.2021.19535\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Ionic liquids are an interesting class of materials that have recently been utilized as chemotherapeutic agents in cancer therapy. Aniline blue, a commonly used biological staining agent, was used as a counter ion to trihexyltetradecylphosphonium, a known cytotoxic cation. A facile, single step ion exchange reaction was performed to synthesize a fluorescent ionic liquid, trihexyltetradecylphosphonium aniline blue. Aqueous nanoparticles of this hydrophobic ionic liquid were prepared using reprecipitationmethod. The newly synthesized ionic liquid and subsequent nanoparticles were characterized using various spectroscopic techniques. Transmission electron microscopy and zeta potential measurements were performed to characterize the nanoparticles' morphology and surface charge. The photophysical properties of the nanoparticles and the parent aniline blue compound were studied using absorption and fluorescence spectroscopy. Cell viability studies were conducted to investigate the cytotoxicity of the newly developed trihexyltetradecylphosphonium aniline blue nanoparticles in human breast epithelial cancer cell line (MCF-7) and its corresponding normal epithelial cell line (MCF-10A) <i>in vitro</i>. The results revealed that the synthesized ionic nanomedicines were more cytotoxic (lower IC<sub>50</sub>) than the parent chemotherapeutic compound in MCF-7 cells. Nanoparticles of the synthesized ionic liquid were also shown to be more stable in both aqueous and cellular media and more selective than parent compounds towards cancer cells.</p>\",\"PeriodicalId\":16417,\"journal\":{\"name\":\"Journal of nanoscience and nanotechnology\",\"volume\":\"21 12\",\"pages\":\"6143-6150\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8919709/pdf/nihms-1783303.pdf\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of nanoscience and nanotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1166/jnn.2021.19535\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of nanoscience and nanotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1166/jnn.2021.19535","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

摘要

离子液体是一类有趣的材料,最近被用作癌症治疗的化疗药物。苯胺蓝,一种常用的生物染色剂,被用作三己基十四烷基磷的反离子,一种已知的细胞毒性阳离子。采用简单的单步离子交换反应合成了荧光离子液体三己基十四烷基磷苯胺蓝。采用再沉淀法制备了该疏水离子液体的水纳米颗粒。利用各种光谱技术对新合成的离子液体及其纳米颗粒进行了表征。通过透射电子显微镜和zeta电位测量来表征纳米颗粒的形貌和表面电荷。利用吸收光谱和荧光光谱研究了纳米粒子及其母体苯胺蓝化合物的光物理性质。采用细胞活力实验研究了新制备的三己基十四烷基磷苯胺蓝纳米颗粒对人乳腺上皮癌细胞(MCF-7)及其相应的正常上皮细胞系(MCF-10A)的体外细胞毒性。结果表明,合成的离子纳米药物对MCF-7细胞的细胞毒性高于母体化疗药物(IC50较低)。合成离子液体的纳米颗粒在水和细胞介质中都表现出更稳定的性能,并且比母体化合物对癌细胞的选择性更强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Tunable Cytotoxicity and Selectivity of Phosphonium Ionic Liquid with Aniline Blue Dye.

Tunable Cytotoxicity and Selectivity of Phosphonium Ionic Liquid with Aniline Blue Dye.

Tunable Cytotoxicity and Selectivity of Phosphonium Ionic Liquid with Aniline Blue Dye.

Ionic liquids are an interesting class of materials that have recently been utilized as chemotherapeutic agents in cancer therapy. Aniline blue, a commonly used biological staining agent, was used as a counter ion to trihexyltetradecylphosphonium, a known cytotoxic cation. A facile, single step ion exchange reaction was performed to synthesize a fluorescent ionic liquid, trihexyltetradecylphosphonium aniline blue. Aqueous nanoparticles of this hydrophobic ionic liquid were prepared using reprecipitationmethod. The newly synthesized ionic liquid and subsequent nanoparticles were characterized using various spectroscopic techniques. Transmission electron microscopy and zeta potential measurements were performed to characterize the nanoparticles' morphology and surface charge. The photophysical properties of the nanoparticles and the parent aniline blue compound were studied using absorption and fluorescence spectroscopy. Cell viability studies were conducted to investigate the cytotoxicity of the newly developed trihexyltetradecylphosphonium aniline blue nanoparticles in human breast epithelial cancer cell line (MCF-7) and its corresponding normal epithelial cell line (MCF-10A) in vitro. The results revealed that the synthesized ionic nanomedicines were more cytotoxic (lower IC50) than the parent chemotherapeutic compound in MCF-7 cells. Nanoparticles of the synthesized ionic liquid were also shown to be more stable in both aqueous and cellular media and more selective than parent compounds towards cancer cells.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of nanoscience and nanotechnology
Journal of nanoscience and nanotechnology 工程技术-材料科学:综合
自引率
0.00%
发文量
0
审稿时长
3.6 months
期刊介绍: JNN is a multidisciplinary peer-reviewed journal covering fundamental and applied research in all disciplines of science, engineering and medicine. JNN publishes all aspects of nanoscale science and technology dealing with materials synthesis, processing, nanofabrication, nanoprobes, spectroscopy, properties, biological systems, nanostructures, theory and computation, nanoelectronics, nano-optics, nano-mechanics, nanodevices, nanobiotechnology, nanomedicine, nanotoxicology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信