在脂肪形成过程中,Staufen1解开二级结构,促进脂肪酸结合蛋白4mrna的翻译。

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM
Xiaodi Liang, Yi Jiao, Xueli Gong, Hao Gu, Nuerbiye Nuermaimaiti, Xuanyu Meng, Dihui Liu, Yaqun Guan
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引用次数: 1

摘要

脂肪形成受遗传相互作用的调控,其中转录后调控起着重要作用。Staufen双链RNA结合蛋白1 (Staufen1或STAU1)在RNA加工和脂肪形成过程中发挥着多种作用。之前,我们发现STAU1的下调在蛋白水平上影响脂肪酸结合蛋白4 (FABP4)的表达,但在mRNA水平上不受影响。本研究旨在确定STAU1调控FABP4表达的机制,解释FABP4 mRNA和蛋白水平的不一致。我们使用RNA干扰,光激活核糖核苷增强交联和免疫沉淀,以及腺相关病毒来检测STAU1在脂肪形成中的功能。我们的研究结果表明,STAU1结合到FABP4的编码序列上,从而通过解开FABP4的双链结构来调节FABP4 mRNA的翻译。此外,STAU1通过调节游离脂肪酸的分泌介导脂肪形成。然而,STAU1敲除降低了脂肪重量/体重比,但不影响血浆甘油三酯水平。这些发现描述了在脂肪形成过程中,stau1介导的FABP4表达在翻译水平上的调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis.

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis.

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis.

Staufen1 unwinds the secondary structure and facilitates the translation of fatty acid binding protein 4 mRNA during adipogenesis.

Adipogenesis is regulated by genetic interactions, in which post-transcriptional regulation plays an important role. Staufen double-stranded RNA binding protein 1 (Staufen1 or STAU1) plays diverse roles in RNA processing and adipogenesis. Previously, we found that the downregulation of STAU1 affects the expression of fatty acid-binding protein 4 (FABP4) at the protein level but not at the mRNA level. This study aimed to determine the mechanism underlying the regulation of FABP4 expression by STAU1, explaining the inconsistency between FABP4 mRNA and protein levels. We used RNA interference, photoactivatable ribonucleoside enhanced cross-linking and immunoprecipitation, and an adeno-associated virus to examine the functions of STAU1 in adipogenesis. Our results indicate that STAU1 binds to the coding sequences of FABP4, thereby regulating the translation of FABP4 mRNA by unwinding the double-stranded structure. Furthermore, STAU1 mediates adipogenesis by regulating the secretion of free fatty acids. However, STAU1 knockdown decreases the fat weight/body weight ratio but does not affect the plasma triglyceride levels. These findings describe the mechanisms involved in STAU1-mediated regulation of FABP4 expression at the translational level during adipogenesis.

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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
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