太空飞行减缓了小鼠视网膜中与DNA甲基组和转录组整体改变相协调的表观遗传时钟。

IF 5.1 4区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Precision Clinical Medicine Pub Date : 2021-05-17 eCollection Date: 2021-06-01 DOI:10.1093/pcmedi/pbab012
Zhong Chen, Seta Stanbouly, Nina C Nishiyama, Xin Chen, Michael D Delp, Hongyu Qiu, Xiao W Mao, Charles Wang
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引用次数: 6

摘要

在长时间的太空飞行中,宇航员的眼睛会出现各种临床异常。这项研究的目的是确定太空飞行是否会诱导视网膜的表观基因组和转录组重编程或改变表观遗传时钟。这些老鼠在国际空间站的动物封闭舱中飞行了37天;地面上的对照动物也在类似的饲养条件下饲养。分离小鼠视网膜,采用深度测序法测定DNA甲基组和转录组。我们发现大量基因在太空飞行中被差异甲基化,而在转录组水平上差异表达的基因较少。与黄斑变性等视网膜疾病相关的几种生物学途径发生了显著改变。我们的研究结果表明,太空飞行使视网膜表观遗传时钟减速。这项研究表明,太空飞行在表观基因组和转录组水平上影响视网膜,这种变化可能涉及宇航员眼睛相关疾病的病因学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spaceflight decelerates the epigenetic clock orchestrated with a global alteration in DNA methylome and transcriptome in the mouse retina.

Astronauts exhibit an assortment of clinical abnormalities in their eyes during long-duration spaceflight. The purpose of this study was to determine whether spaceflight induces epigenomic and transcriptomic reprogramming in the retina or alters the epigenetic clock. The mice were flown for 37 days in animal enclosure modules on the International Space Station; ground-based control animals were maintained under similar housing conditions. Mouse retinas were isolated and both DNA methylome and transcriptome were determined by deep sequencing. We found that a large number of genes were differentially methylated with spaceflight, whereas there were fewer differentially expressed genes at the transcriptome level. Several biological pathways involved in retinal diseases such as macular degeneration were significantly altered. Our results indicated that spaceflight decelerated the retinal epigenetic clock. This study demonstrates that spaceflight impacts the retina at the epigenomic and transcriptomic levels, and such changes could be involved in the etiology of eye-related disorders among astronauts.

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来源期刊
Precision Clinical Medicine
Precision Clinical Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
10.80
自引率
0.00%
发文量
26
审稿时长
5 weeks
期刊介绍: Precision Clinical Medicine (PCM) is an international, peer-reviewed, open access journal that provides timely publication of original research articles, case reports, reviews, editorials, and perspectives across the spectrum of precision medicine. The journal's mission is to deliver new theories, methods, and evidence that enhance disease diagnosis, treatment, prevention, and prognosis, thereby establishing a vital communication platform for clinicians and researchers that has the potential to transform medical practice. PCM encompasses all facets of precision medicine, which involves personalized approaches to diagnosis, treatment, and prevention, tailored to individual patients or patient subgroups based on their unique genetic, phenotypic, or psychosocial profiles. The clinical conditions addressed by the journal include a wide range of areas such as cancer, infectious diseases, inherited diseases, complex diseases, and rare diseases.
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