鸡胚绒毛膜尿囊膜模型:一种体外和体内实验研究方法。

IF 3.5 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ana Isabel Fraguas-Sánchez, Cristina Martín-Sabroso, Ana Isabel Torres-Suárez
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引用次数: 7

摘要

背景:鸡绒毛膜尿囊膜(CAM)模型作为一种替代或补充动物模型的体内实验,在制药和生物学研究中引起了极大的兴趣。传统上,CAM测定已广泛用于进行一些毒理学研究,特别是评估新药物和配方的皮肤,眼睛和胚胎毒性,以及进行血管生成研究。由于可以在CAM上生成肿瘤,该模型也成为评估不同肿瘤转移潜力和测试新型抗癌疗法体内疗效的绝佳策略。此外,近年来,它在其他研究领域的应用也大大增加,包括新的抗感染剂的评价,生物分布研究的发展和组织工程研究。目的:本文综述了CAM模型在制药和生物学研究中的应用,特别是在测试新药和配方的毒性以及新型抗癌和抗感染疗法的生物分布和疗效方面的应用,并分析了其与动物模型相比的优缺点。结论:鸡绒毛膜尿囊膜模型在癌症、毒理学、生物分布研究和抗感染治疗等研究领域具有重要的应用价值。事实上,它已经成为介于体外实验和动物研究之间的中间阶段,在毒理学研究(皮肤和眼毒性)的情况下,它甚至取代了动物模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Chick Embryo Chorioallantoic Membrane Model: A Research Approach for Ex Vivo and In Vivo Experiments.

Background: The chick chorioallantoic membrane (CAM) model has attracted a great deal of interest in pharmaceutical and biological research as an alternative or complimentary in vivo assay to animal models. Traditionally, CAM assay has been widely used to perform some toxicological studies, specifically to evaluate the skin, ocular and embryo toxicity of new drugs and formulations, and to perform angiogenesis studies. Due to the possibility to generate the tumors onto the CAM, this model has also become an excellent strategy to evaluate the metastatic potential of different tumours and to test the efficacy of novel anticancer therapies in vivo. Moreover, in the recent years, its use has considerably grown in other research areas, including the evaluation of new anti-infective agents, the development of biodistribution studies and in tissue engineering research.

Objective: This manuscript provides a critical overview of the use of CAM model in pharmaceutical and biological research, especially to test the toxicity of new drugs and formulations and the biodistribution and the efficacy of novel anticancer and antiinfective therapies, analyzing its advantages and disadvantages in comparison to animal models.

Conclusion: The chick chorioallantoic membrane model shows a great utility in several research areas, such as cancer, toxicology, biodistribution studies and anti-infective therapies. In fact, it has become an intermediate stage between in vitro experiments and animal studies, and, in the case of toxicological studies (skin and ocular toxicity), it has even replaced the animal models.

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来源期刊
Current medicinal chemistry
Current medicinal chemistry 医学-生化与分子生物学
CiteScore
8.60
自引率
2.40%
发文量
468
审稿时长
3 months
期刊介绍: Aims & Scope Current Medicinal Chemistry covers all the latest and outstanding developments in medicinal chemistry and rational drug design. Each issue contains a series of timely in-depth reviews and guest edited thematic issues written by leaders in the field covering a range of the current topics in medicinal chemistry. The journal also publishes reviews on recent patents. Current Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments.
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