依赖于 Hrd1 的 GPI 跨酰胺酶复合物未组装 PIGK 亚基降解。

IF 2 4区生物学 Q4 CELL BIOLOGY

Cell structure and function Pub Date : 2021-09-03 Epub Date: 2021-06-30 DOI:10.1247/csf.21019

Kohei Kawaguchi, Miki Yamamoto-Hino, Yoshiko Murakami, Taroh Kinoshita, Satoshi Goto

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引用次数: 1

摘要

糖基磷脂酰肌醇(GPI)锚定蛋白在转录后被GPI修饰并锚定在质膜上。GPI通过由PIGT、PIGK、GPAA1、PIGU和pig组成的GPI转氨酶复合物附着在内质网中的新生蛋白上。其中，PIGK是一个催化亚基，没有PIGT是不稳定的。本研究探讨了未整合到复合物中的未组装的PIGK被降解的途径。我们发现未组装的PIGK是通过蛋白酶体依赖途径降解的，Hrd1(也称为SYVN1)是一种参与内质网相关降解途径的泛素连接酶，负责未组装的PIGK的降解。关键词:糖基磷脂酰肌醇，GPI转氨酶复合物，蛋白质稳定性，转氨化，ERAD

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Hrd1-dependent Degradation of the Unassembled PIGK Subunit of the GPI Transamidase Complex.

Glycosylphosphatidylinositol (GPI)-anchored proteins are post-transcriptionally modified with GPI and anchored to the plasma membrane. GPI is attached to nascent proteins in the endoplasmic reticulum by the GPI transamidase complex, which consists of PIGT, PIGK, GPAA1, PIGU, and PIGS. Of these, PIGK is a catalytic subunit that is unstable without PIGT. This study investigated the pathway by which unassembled PIGK not incorporated into the complex is degraded. We showed that unassembled PIGK was degraded via the proteasome-dependent pathway and that Hrd1 (also known as SYVN1), a ubiquitin ligase involved in the endoplasmic reticulum-associated degradation pathway, was responsible for degradation of unassembled PIGK.Key words: Glycosylphosphatidylinositol, GPI transamidase complex, protein stability, transamidation, ERAD.

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来源期刊

Cell structure and function 生物-细胞生物学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Cell Structure and Function is a fully peer-reviewed, fully Open Access journal. As the official English-language journal of the Japan Society for Cell Biology, it is published continuously online and biannually in print. Cell Structure and Function publishes important, original contributions in all areas of molecular and cell biology. The journal welcomes the submission of manuscripts on research areas such as the cell nucleus, chromosomes, and gene expression; the cytoskeleton and cell motility; cell adhesion and the extracellular matrix; cell growth, differentiation and death; signal transduction; the protein life cycle; membrane traffic; and organelles.