单细胞电感耦合等离子体质谱法测定单个酿酒酵母细胞中元素分布及元素浓度评价。

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metallomics Pub Date : 2021-06-21 DOI:10.1093/mtomcs/mfab032
Wen Qin, Hans-Joachim Stärk, Susann Müller, Thorsten Reemtsma, Stephan Wagner
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引用次数: 5

摘要

利用电感耦合等离子体质谱法(SC-ICP-MS)进行单细胞分析是一种获得单细胞元素含量和分布的定性和定量信息的方法。在不同磷浓度(0、7、14 mM)培养基中培养不同生长阶段的酵母细胞,分析了6种内在靶元素对细胞生长和组成的影响。SC-ICP-MS结果与酸消化法比较,平均比值为0.81。该法对Mg、P、K、Mn、Cu、Zn的检出限分别为0.08、2.54、12.5、0.02、0.02和0.08 fg cell-1。在指数生长期,细胞的元素含量比稳定生长期高,大多数元素的分布范围更广,细胞尺寸也更大。无磷条件使固定生长期单细胞平均磷含量从650降低到80 fg。缺磷导致细胞内P、K和Cu浓度降低,48 h后Zn浓度升高。Mg浓度维持在~ 0.11 fgµm-3, 48 h后在三种条件下没有显著变化。因此,Mg含量成功地用于估计单个酵母细胞内其他固有元素的浓度。SC-ICP-MS适用于测定单个酵母细胞中的目标元素,并允许研究细胞组成的异质性以及应激源对元素含量、分布和内在元素浓度的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of elemental distribution and evaluation of elemental concentration in single Saccharomyces cerevisiae cells using single cell-inductively coupled plasma mass spectrometry.

Single-cell analysis using inductively coupled plasma mass spectrometry (SC-ICP-MS) is a method to obtain qualitative and quantitative information of the elemental content and distribution of single cells. Six intrinsic target elements were analyzed in yeast cells at different cell growth phases cultured in medium with different phosphorus concentrations (0, 7, 14 mM) to study its effect on cell growth and composition. SC-ICP-MS results were compared with those obtained by the acid digestion and the average ratio was 0.81. The limits of detection of this method were 0.08, 2.54, 12.5, 0.02, 0.02, and 0.08 fg cell-1 for Mg, P, K, Mn, Cu, and Zn, respectively. During the exponential growth phase, the cells exhibited higher elemental contents, wider distribution for most elements, and larger cell size in comparison to the stationary growth phase. Phosphorus-free conditions reduced the average P content in single cells of stationary growth phase from 650 to 80 fg. Phosphorus deficiency led to decreasing intracellular concentrations not only of P but also of K and Cu, and to increasing Zn concentration after 48 h. Mg maintained its concentration at ∼0.11 fg µm-3 and did not change significantly under the three investigated conditions after 48 h. Accordingly, Mg content was successfully used to estimate the intracellular concentration of other intrinsic elements in single yeast cells. SC-ICP-MS is suited to determine target elements in single yeast cells, and allows the study of heterogeneity of cell composition and effects of stressors on the elemental content, distribution, and concentrations of intrinsic elements.

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来源期刊
Metallomics
Metallomics 生物-生化与分子生物学
CiteScore
7.00
自引率
5.90%
发文量
87
审稿时长
1 months
期刊介绍: Global approaches to metals in the biosciences
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