子痫前期母体微血管内皮细胞中肿瘤坏死因子α表达升高。

IF 1.5 4区医学 Q3 OBSTETRICS & GYNECOLOGY

Hypertension in Pregnancy Pub Date : 2021-08-01 Epub Date: 2021-05-12 DOI:10.1080/10641955.2021.1921794

Dennis K Lee, Ori Nevo

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引用次数: 2

摘要

目的:探讨肿瘤坏死因子α (TNFα)在子痫前期母体内皮细胞中的表达及其影响。方法:采用RT-qPCR方法检测重度子痫前期(PE)和正常妊娠患者分离的原代微血管内皮细胞(MVEC)在TNFα和下游信号抑制剂治疗或不治疗的情况下的表达水平。结果:PE MVEC的TNFα基础表达增加。tnf - α处理增加了MVEC中tnf - α、VCAM和endocan的表达。结论:tnf - α在PE MVEC中表达升高，外源tnf - α处理可改变其基因表达。

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Tumor necrosis factor alpha expression is increased in maternal microvascular endothelial cells in preeclampsia.

Objective: The aim of this study was to explore the expression and effects of tumor necrosis factor alpha (TNFα) in maternal endothelial cells in preeclampsia (PE).Methods: Expression levels in primary microvascular endothelial cells (MVEC) isolated from patients with severe preeclampsia (PE) and normal pregnancies were determined by RT-qPCR with or without treatment of TNFα and inhibitors for downstream signaling.Results: PE MVEC exhibited increased basal TNFα expression. TNFα treatment increased TNFα, VCAM, and endocan expression in MVEC.Conclusion: TNFα expression is increased in PE MVEC and the treatment of these cells with exogenous TNFα modifies their gene expression.

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来源期刊

Hypertension in Pregnancy 医学-妇产科学

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Hypertension in Pregnancy is a refereed journal in the English language which publishes data pertaining to human and animal hypertension during gestation. Contributions concerning physiology of circulatory control, pathophysiology, methodology, therapy or any other material relevant to the relationship between elevated blood pressure and pregnancy are acceptable. Published material includes original articles, clinical trials, solicited and unsolicited reviews, editorials, letters, and other material deemed pertinent by the editors.