Hassan Rakhshandeh, Amirhossein Heidari, Ali Mohammad Pourbagher-Shahri, Roghayeh Rashidi, Fatemeh Forouzanfar
{"title":"苦艾水醇提取物对戊巴比妥治疗小鼠的催眠作用。","authors":"Hassan Rakhshandeh, Amirhossein Heidari, Ali Mohammad Pourbagher-Shahri, Roghayeh Rashidi, Fatemeh Forouzanfar","doi":"10.1155/2021/5521019","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Current drugs used in the management of insomnia are associated with side effects. The use of medicinal herbs for insomnia treatment has recently been suggested.</p><p><strong>Objective: </strong>The present study aimed to determine the hypnotic activity of the hydroalcoholic extract of <i>Artemisia absinthium (A</i>. <i>absinthium)</i> in mice.</p><p><strong>Method: </strong>The toxicity of <i>A</i>. <i>absinthium</i> extract is assessed by their lethal dose 50% (LD50), and cytotoxicity evaluation was also done with PC12 cell lines by MTT assay. <i>A</i>. <i>absinthium</i> extract (25, 50, 100, and 200 mg/kg) and 3 fractions (<i>n</i>-butanol fraction (NBF), ethyl acetate fraction (EAF), and aqueous fraction (AQF)) were administered intraperitoneally30 minutes before 30 mg/kg pentobarbital intraperitoneal injection; after that, the sleeping time and sleep latency were recorded.</p><p><strong>Results: </strong>The LD50 value was 2.4 g/kg. The extracts tested showed no negative effect on the proliferation of PC12 cells. <i>A</i>. <i>absinthium</i> extract increased the duration of pentobarbital-induced sleep at doses of 100 and 200 mg/kg (<i>P</i> < 0.01-<i>P</i> < 0.001). Similarly, AQF, EAF, and NBF at 200 mg/kg could increase sleep duration (<i>P</i> < 0.05). The sleep latency was decreased by <i>A</i>. <i>absinthium</i> extract at doses of 100 and 200 mg/kg (<i>P</i> < 0.05-<i>P</i> < 0.01), AQF (<i>P</i> < 0.05), and EAF (<i>P</i> < 0.05). Besides, flumazenil reversed the hypnotic effect of <i>A</i>. <i>absinthium</i> extract (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong><i>A</i>. <i>absinthium</i> extract probably demonstrated sleep-enhancing effects by regulating GABAergic system.</p>","PeriodicalId":19124,"journal":{"name":"Neurology Research International","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2021-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084640/pdf/","citationCount":"6","resultStr":"{\"title\":\"Hypnotic Effect of <i>A</i>. <i>absinthium</i> Hydroalcoholic Extract in Pentobarbital-Treated Mice.\",\"authors\":\"Hassan Rakhshandeh, Amirhossein Heidari, Ali Mohammad Pourbagher-Shahri, Roghayeh Rashidi, Fatemeh Forouzanfar\",\"doi\":\"10.1155/2021/5521019\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Current drugs used in the management of insomnia are associated with side effects. The use of medicinal herbs for insomnia treatment has recently been suggested.</p><p><strong>Objective: </strong>The present study aimed to determine the hypnotic activity of the hydroalcoholic extract of <i>Artemisia absinthium (A</i>. <i>absinthium)</i> in mice.</p><p><strong>Method: </strong>The toxicity of <i>A</i>. <i>absinthium</i> extract is assessed by their lethal dose 50% (LD50), and cytotoxicity evaluation was also done with PC12 cell lines by MTT assay. <i>A</i>. <i>absinthium</i> extract (25, 50, 100, and 200 mg/kg) and 3 fractions (<i>n</i>-butanol fraction (NBF), ethyl acetate fraction (EAF), and aqueous fraction (AQF)) were administered intraperitoneally30 minutes before 30 mg/kg pentobarbital intraperitoneal injection; after that, the sleeping time and sleep latency were recorded.</p><p><strong>Results: </strong>The LD50 value was 2.4 g/kg. The extracts tested showed no negative effect on the proliferation of PC12 cells. <i>A</i>. <i>absinthium</i> extract increased the duration of pentobarbital-induced sleep at doses of 100 and 200 mg/kg (<i>P</i> < 0.01-<i>P</i> < 0.001). Similarly, AQF, EAF, and NBF at 200 mg/kg could increase sleep duration (<i>P</i> < 0.05). The sleep latency was decreased by <i>A</i>. <i>absinthium</i> extract at doses of 100 and 200 mg/kg (<i>P</i> < 0.05-<i>P</i> < 0.01), AQF (<i>P</i> < 0.05), and EAF (<i>P</i> < 0.05). Besides, flumazenil reversed the hypnotic effect of <i>A</i>. <i>absinthium</i> extract (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong><i>A</i>. <i>absinthium</i> extract probably demonstrated sleep-enhancing effects by regulating GABAergic system.</p>\",\"PeriodicalId\":19124,\"journal\":{\"name\":\"Neurology Research International\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2021-04-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8084640/pdf/\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neurology Research International\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2021/5521019\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"NEUROSCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurology Research International","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2021/5521019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
Hypnotic Effect of A. absinthium Hydroalcoholic Extract in Pentobarbital-Treated Mice.
Background: Current drugs used in the management of insomnia are associated with side effects. The use of medicinal herbs for insomnia treatment has recently been suggested.
Objective: The present study aimed to determine the hypnotic activity of the hydroalcoholic extract of Artemisia absinthium (A. absinthium) in mice.
Method: The toxicity of A. absinthium extract is assessed by their lethal dose 50% (LD50), and cytotoxicity evaluation was also done with PC12 cell lines by MTT assay. A. absinthium extract (25, 50, 100, and 200 mg/kg) and 3 fractions (n-butanol fraction (NBF), ethyl acetate fraction (EAF), and aqueous fraction (AQF)) were administered intraperitoneally30 minutes before 30 mg/kg pentobarbital intraperitoneal injection; after that, the sleeping time and sleep latency were recorded.
Results: The LD50 value was 2.4 g/kg. The extracts tested showed no negative effect on the proliferation of PC12 cells. A. absinthium extract increased the duration of pentobarbital-induced sleep at doses of 100 and 200 mg/kg (P < 0.01-P < 0.001). Similarly, AQF, EAF, and NBF at 200 mg/kg could increase sleep duration (P < 0.05). The sleep latency was decreased by A. absinthium extract at doses of 100 and 200 mg/kg (P < 0.05-P < 0.01), AQF (P < 0.05), and EAF (P < 0.05). Besides, flumazenil reversed the hypnotic effect of A. absinthium extract (P < 0.05).
Conclusion: A. absinthium extract probably demonstrated sleep-enhancing effects by regulating GABAergic system.
期刊介绍：
Neurology Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies focusing on diseases of the nervous system, as well as normal neurological functioning. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
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