尼日利亚贡贝州tudun-wada健康人群中多重耐药pantonvalentine白细胞介素阳性金黄色葡萄球菌的鼻腔携带。

Q4 Medicine
African Journal of Infectious Diseases Pub Date : 2020-12-14 eCollection Date: 2021-01-01 DOI:10.21010/ajid.v15i1.3
Adebola Onanuga, Ocholi Jonathan Adamu, Babatunde Odetoyin, Jabir Adamu Hamza
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引用次数: 2

摘要

背景:产生潘通-瓦伦丁白葡萄球菌(PVL)的金黄色葡萄球菌菌株与严重的社区相关侵袭性感染有关,其日益增加的多药耐药性是一个主要的全球卫生问题。因此,我们调查了尼日利亚贡贝州Tundu-Wada健康成人鼻腔金黄色葡萄球菌PVL基因的流行率和耐药性谱。方法与材料:采集健康成人鼻标本262份,进行培养。采用标准形态学和生化方法鉴定分离株为金黄色葡萄球菌,并对其16S rRNA基因进行PCR扩增。采用圆盘扩散法进行药敏试验,PCR检测mecA和PVL基因的存在。结果:金黄色葡萄球菌鼻腔定植率为17.6%。分离株中溶血素和生物膜产生率分别为25株(54.3%)和42株(91.3%)。分别有2株(4.3%)和5株(10.9%)具有mecA和PVL基因,但均不含这两个基因。所有菌株均对阿莫西林耐药,但对庆大霉素高度敏感(93.7%)。菌株多重耐药(MDR)发生率为45.7%,产生pvl的菌株均为MDR, 1株携带mecA基因的菌株表现为广泛耐药(XDR)。结论:这是尼日利亚东北部贡贝健康成人中产生耐多药pvl的金黄色葡萄球菌鼻腔定植的第一份报告。这项研究强调了对健康人群进行常规监测的重要性,为控制社区内毒性多重耐药生物的传播提供了有用的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

NASAL CARRIAGE OF MULTI-DRUG RESISTANT PANTON VALENTINE LEUKOCIDIN POSITIVE <i>STAPHYLOCOCCUS AUREUS</i> IN HEALTHY INDIVIDUALS OF TUDUN-WADA, GOMBE STATE, NIGERIA.

NASAL CARRIAGE OF MULTI-DRUG RESISTANT PANTON VALENTINE LEUKOCIDIN POSITIVE <i>STAPHYLOCOCCUS AUREUS</i> IN HEALTHY INDIVIDUALS OF TUDUN-WADA, GOMBE STATE, NIGERIA.

NASAL CARRIAGE OF MULTI-DRUG RESISTANT PANTON VALENTINE LEUKOCIDIN POSITIVE <i>STAPHYLOCOCCUS AUREUS</i> IN HEALTHY INDIVIDUALS OF TUDUN-WADA, GOMBE STATE, NIGERIA.

NASAL CARRIAGE OF MULTI-DRUG RESISTANT PANTON VALENTINE LEUKOCIDIN POSITIVE STAPHYLOCOCCUS AUREUS IN HEALTHY INDIVIDUALS OF TUDUN-WADA, GOMBE STATE, NIGERIA.

Background: Panton-Valentine Leucocidin (PVL)-producing Staphylococcus aureus strains have been implicated in serious community-associated invasive infections and their increasing multidrug resistance is a major global health concern. Thus, we investigated the prevalence of the PVL gene and the antimicrobial resistance profile of nasal S. aureus isolates from healthy adults in Tundu-Wada, Gombe State of Nigeria.

Methods and materials: A total of 262 nasal samples from healthy adults were obtained and cultured. The isolates were identified as S. aureus by standard morphological and biochemical methods alongside with the Polymerase Chain Reaction (PCR) amplification of their 16S rRNA gene. Antimicrobial susceptibility testing was performed by the disc diffusion technique and the presence of mecA and PVL genes was determined by PCR analysis.

Results: The overall nasal colonization of S. aureus was 17.6%. The prevalence of haemolysin and biofilm production among the isolates was 25(54.3%) and 42(91.3%), respectively. Only 2(4.3%) and 5(10.9%) possessed mecA and PVL genes respectively but none of the isolates harboured these two genes. All the isolates were resistant to amoxicillin but were highly susceptible (93.7%) to gentamicin. The prevalence of multi-drug resistance (MDR) among the isolates was M 45.7% and all PVL-producing isolates were MDR while one of the isolates with mecA gene exhibited extensive-drug resistance (XDR).

Conclusion: This is the first report of nasal colonization of MDR PVL-producing S. aureus in healthy adults in Gombe, Northeastern Nigeria. This study highlights the importance of routine surveillance of healthy populations to provide useful strategies for controlling the spread of virulent multidrug-resistant organisms within the community.

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来源期刊
African Journal of Infectious Diseases
African Journal of Infectious Diseases Medicine-Infectious Diseases
CiteScore
1.60
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0.00%
发文量
32
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