Adebola Onanuga, Ocholi Jonathan Adamu, Babatunde Odetoyin, Jabir Adamu Hamza
{"title":"尼日利亚贡贝州tudun-wada健康人群中多重耐药pantonvalentine白细胞介素阳性金黄色葡萄球菌的鼻腔携带。","authors":"Adebola Onanuga, Ocholi Jonathan Adamu, Babatunde Odetoyin, Jabir Adamu Hamza","doi":"10.21010/ajid.v15i1.3","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Panton-Valentine Leucocidin (PVL)-producing <i>Staphylococcus aureus</i> strains have been implicated in serious community-associated invasive infections and their increasing multidrug resistance is a major global health concern. Thus, we investigated the prevalence of the PVL gene and the antimicrobial resistance profile of nasal <i>S. aureus</i> isolates from healthy adults in Tundu-Wada, Gombe State of Nigeria.</p><p><strong>Methods and materials: </strong>A total of 262 nasal samples from healthy adults were obtained and cultured. The isolates were identified as <i>S. aureus</i> by standard morphological and biochemical methods alongside with the Polymerase Chain Reaction (PCR) amplification of their <i>16S rRNA</i> gene. Antimicrobial susceptibility testing was performed by the disc diffusion technique and the presence of <i>mecA</i> and PVL genes was determined by PCR analysis.</p><p><strong>Results: </strong>The overall nasal colonization of <i>S. aureus</i> was 17.6%. The prevalence of haemolysin and biofilm production among the isolates was 25(54.3%) and 42(91.3%), respectively. Only 2(4.3%) and 5(10.9%) possessed <i>mecA</i> and PVL genes respectively but none of the isolates harboured these two genes. All the isolates were resistant to amoxicillin but were highly susceptible (93.7%) to gentamicin. The prevalence of multi-drug resistance (MDR) among the isolates was M 45.7% and all PVL-producing isolates were MDR while one of the isolates with <i>mecA</i> gene exhibited extensive-drug resistance (XDR).</p><p><strong>Conclusion: </strong>This is the first report of nasal colonization of MDR PVL-producing <i>S. aureus</i> in healthy adults in Gombe, Northeastern Nigeria. This study highlights the importance of routine surveillance of healthy populations to provide useful strategies for controlling the spread of virulent multidrug-resistant organisms within the community.</p>","PeriodicalId":39108,"journal":{"name":"African Journal of Infectious Diseases","volume":"15 1","pages":"24-33"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047283/pdf/","citationCount":"2","resultStr":"{\"title\":\"NASAL CARRIAGE OF MULTI-DRUG RESISTANT PANTON VALENTINE LEUKOCIDIN POSITIVE <i>STAPHYLOCOCCUS AUREUS</i> IN HEALTHY INDIVIDUALS OF TUDUN-WADA, GOMBE STATE, NIGERIA.\",\"authors\":\"Adebola Onanuga, Ocholi Jonathan Adamu, Babatunde Odetoyin, Jabir Adamu Hamza\",\"doi\":\"10.21010/ajid.v15i1.3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Panton-Valentine Leucocidin (PVL)-producing <i>Staphylococcus aureus</i> strains have been implicated in serious community-associated invasive infections and their increasing multidrug resistance is a major global health concern. Thus, we investigated the prevalence of the PVL gene and the antimicrobial resistance profile of nasal <i>S. aureus</i> isolates from healthy adults in Tundu-Wada, Gombe State of Nigeria.</p><p><strong>Methods and materials: </strong>A total of 262 nasal samples from healthy adults were obtained and cultured. The isolates were identified as <i>S. aureus</i> by standard morphological and biochemical methods alongside with the Polymerase Chain Reaction (PCR) amplification of their <i>16S rRNA</i> gene. Antimicrobial susceptibility testing was performed by the disc diffusion technique and the presence of <i>mecA</i> and PVL genes was determined by PCR analysis.</p><p><strong>Results: </strong>The overall nasal colonization of <i>S. aureus</i> was 17.6%. The prevalence of haemolysin and biofilm production among the isolates was 25(54.3%) and 42(91.3%), respectively. Only 2(4.3%) and 5(10.9%) possessed <i>mecA</i> and PVL genes respectively but none of the isolates harboured these two genes. All the isolates were resistant to amoxicillin but were highly susceptible (93.7%) to gentamicin. The prevalence of multi-drug resistance (MDR) among the isolates was M 45.7% and all PVL-producing isolates were MDR while one of the isolates with <i>mecA</i> gene exhibited extensive-drug resistance (XDR).</p><p><strong>Conclusion: </strong>This is the first report of nasal colonization of MDR PVL-producing <i>S. aureus</i> in healthy adults in Gombe, Northeastern Nigeria. This study highlights the importance of routine surveillance of healthy populations to provide useful strategies for controlling the spread of virulent multidrug-resistant organisms within the community.</p>\",\"PeriodicalId\":39108,\"journal\":{\"name\":\"African Journal of Infectious Diseases\",\"volume\":\"15 1\",\"pages\":\"24-33\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8047283/pdf/\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"African Journal of Infectious Diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21010/ajid.v15i1.3\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Journal of Infectious Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21010/ajid.v15i1.3","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
NASAL CARRIAGE OF MULTI-DRUG RESISTANT PANTON VALENTINE LEUKOCIDIN POSITIVE STAPHYLOCOCCUS AUREUS IN HEALTHY INDIVIDUALS OF TUDUN-WADA, GOMBE STATE, NIGERIA.
Background: Panton-Valentine Leucocidin (PVL)-producing Staphylococcus aureus strains have been implicated in serious community-associated invasive infections and their increasing multidrug resistance is a major global health concern. Thus, we investigated the prevalence of the PVL gene and the antimicrobial resistance profile of nasal S. aureus isolates from healthy adults in Tundu-Wada, Gombe State of Nigeria.
Methods and materials: A total of 262 nasal samples from healthy adults were obtained and cultured. The isolates were identified as S. aureus by standard morphological and biochemical methods alongside with the Polymerase Chain Reaction (PCR) amplification of their 16S rRNA gene. Antimicrobial susceptibility testing was performed by the disc diffusion technique and the presence of mecA and PVL genes was determined by PCR analysis.
Results: The overall nasal colonization of S. aureus was 17.6%. The prevalence of haemolysin and biofilm production among the isolates was 25(54.3%) and 42(91.3%), respectively. Only 2(4.3%) and 5(10.9%) possessed mecA and PVL genes respectively but none of the isolates harboured these two genes. All the isolates were resistant to amoxicillin but were highly susceptible (93.7%) to gentamicin. The prevalence of multi-drug resistance (MDR) among the isolates was M 45.7% and all PVL-producing isolates were MDR while one of the isolates with mecA gene exhibited extensive-drug resistance (XDR).
Conclusion: This is the first report of nasal colonization of MDR PVL-producing S. aureus in healthy adults in Gombe, Northeastern Nigeria. This study highlights the importance of routine surveillance of healthy populations to provide useful strategies for controlling the spread of virulent multidrug-resistant organisms within the community.